Salt-adaptation of tobacco BY2 cells induces change in glycoform of N-glycans: Enhancement of exo- and endo-glycosidase activities by salt-adaptation

Yoshinobu Kimura, Takao Watanabe, Mariko Kimura, Megumi Maeda, Yoshiyuki Murata, Kazuhito Fujiyama

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3 Citations (Scopus)

Abstract

In this report, we describe that a salt adaptation of plant cells induces glycoform changes in N-glycoproteins. Intracellular and cell-wall glycopeptides were prepared from glycoproteins expressed in wild-type BY2 cells and salt-adapted cells. N-Glycans were liberated from those glycopeptides by hydrazinolysis, and the released oligosaccharides were N-acetylated and pyridylaminated. The structures of pyridylaminated (PA-) N-glycans were analyzed by a combination of two-dimensional sugar-chain mapping, MS analysis, and exoglycosidase digestion. In both wild-type cells and salt-adapted cells, the plant complex type structure was predominant among N-glycans expressed on glycoproteins, but we found that the Man2Xyl1Fuc1GlcNAc2 structure was significantly expressed on intracellular and cell-wall glycoproteins of the salt-adapted cells. Furthermore, enhancement of the specific activities of α-mannosidase and β-N-acetylglucosaminidase was observed in the salt-adapted BY2 cells, suggesting that the glycoform changes are due to changes in glycosidase activities.

Original languageEnglish
Pages (from-to)514-522
Number of pages9
JournalBioscience, Biotechnology and Biochemistry
Volume72
Issue number2
DOIs
Publication statusPublished - Mar 13 2008

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Keywords

  • Glycoform change
  • N-glycan
  • Plant glycoprotein
  • Salt-adaptation
  • Tobacco BY2 cells

ASJC Scopus subject areas

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

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