Salt adaptation of K+ channels in the plasma membrane of tobacco cells in suspension culture

Yoshiyuki Murata, Ichiro Obi, Manabu Yoshihashi, Tokuji Ikeda, Tadaaki Kakutani

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

The patch-clamp technique was used to study and compare the characteristics of cation channels in the plasma membrane of cultured lines of tobacco (Nicotiana tabacum L. cv. Bright Yellow-2) cells that were unadapted (NaCl-unadapted cells) and adapted to 50 and 100 mM NaCl (Na50-adapted and Na100-adapted cells). In these three types of tobacco cell, the outward whole-cell current activated by depolarization was dominated mainly by the activity of the outward rectifying K+ channels with a single-channel conductance of 20 pS. The steady-state amplitude of the outward whole-cell currents at all the positive potentials examined decreased in the following order: NaCl-unadapted cells>Na50-adapted cells>Na100-adapted cells. There were no significant differences between the NaCl-unadapted and the Na50-adapted cells in terms of the ratio of permeabilities of these channels to K+ and Na+ ions. Furthermore, no significant differences in terms of the single-channel conductance of these channels were observed among the NaCl-unadapted, the Na50-adapted and the Na100-adapted cells. These observations suggest that adaptation to salinity of tobacco cells in suspension results in reduced permeability of the K+ channels to both K+ and Na+ ions, without any change in the K+/Na+ selectivity and single-channel conductance of these channels.

Original languageEnglish
Pages (from-to)637-644
Number of pages8
JournalPlant and Cell Physiology
Volume35
Issue number4
Publication statusPublished - 1994
Externally publishedYes

Fingerprint

K+ Channel
Suspension Culture
Plasma Membrane
Tobacco
potassium channels
Cell membranes
tobacco
Cell culture
Salt
cell suspension culture
Suspensions
plasma membrane
Salts
Cell Membrane
membrane
salt
salts
plasma
Cell
Depolarization

Keywords

  • K+ channel
  • Patch clamp
  • Plasma membrane
  • Salt stress
  • Tobacco suspension cell

ASJC Scopus subject areas

  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Ecology
  • Cell Biology
  • Physiology
  • Plant Science

Cite this

Salt adaptation of K+ channels in the plasma membrane of tobacco cells in suspension culture. / Murata, Yoshiyuki; Obi, Ichiro; Yoshihashi, Manabu; Ikeda, Tokuji; Kakutani, Tadaaki.

In: Plant and Cell Physiology, Vol. 35, No. 4, 1994, p. 637-644.

Research output: Contribution to journalArticle

Murata, Y, Obi, I, Yoshihashi, M, Ikeda, T & Kakutani, T 1994, 'Salt adaptation of K+ channels in the plasma membrane of tobacco cells in suspension culture', Plant and Cell Physiology, vol. 35, no. 4, pp. 637-644.
Murata, Yoshiyuki ; Obi, Ichiro ; Yoshihashi, Manabu ; Ikeda, Tokuji ; Kakutani, Tadaaki. / Salt adaptation of K+ channels in the plasma membrane of tobacco cells in suspension culture. In: Plant and Cell Physiology. 1994 ; Vol. 35, No. 4. pp. 637-644.
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