Saliva secretion in engrafted mouse bioengineered salivary glands using taste stimulation

Miho Ogawa, Kentaro Yamashita, Minori Niikura, Kei Nakajima, Koh ei Toyoshima, Masamitsu Oshima, Takashi Tsuji

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)

Abstract

Purpose: The aim of this study was to compare saliva flow and protein composition induced using five basic taste stimulations between natural and bioengineered salivary glands. Materials and methods: We developed a mouse saliva secretion model using taste stimulation and analyzed the saliva secretion from natural and bioengineered salivary glands using an assay. The protein components and alpha-amylase in the natural and bioengineered saliva were analyzed by gel electrophoresis and Western blotting. Results: The salivary flow responses induced by sour (citric acid) and bitter (quinine-HCl) stimuli were significantly high in the natural and bioengineered salivary glands. Although the protein concentrations in the natural and bioengineered saliva induced using five basic taste stimulations were similar, the protein composition and the amylase concentration in the natural saliva after taste stimulation had different profiles. Sympathetic and non-sympathetic nerves were observed around the acini and ducts in the natural and bioengineered salivary glands. However, the frequency of neuropeptide Y-positive sympathetic nerves in the bioengineered gland was relatively high compared to that in the natural gland. Conclusions: These results suggest that the signal balance between the sympathetic and parasympathetic components of the efferent nerves in an engrafted bioengineered salivary gland may differ from that in a natural salivary gland.

Original languageEnglish
Pages (from-to)17-25
Number of pages9
JournalJournal of Prosthodontic Research
Volume58
Issue number1
DOIs
Publication statusPublished - Jan 1 2014

Keywords

  • Bioengineered salivary gland
  • Organ regeneration
  • Saliva
  • Taste stimulation

ASJC Scopus subject areas

  • Oral Surgery
  • Dentistry (miscellaneous)

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