Salinibacter sensory rhodopsin: Sensory rhodopsin I-like protein from a eubacterium

Tomomi Kitajima-Ihara, Yuji Furutani, Daisuke Suzuki, Kunio Ihara, Hideki Kandori, Michio Homma, Yuki Sudo

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Abstract

Halobacterium salinarum sensory rhodopsin I (HsSRI), a dual receptor regulating both negative and positive phototaxis in haloarchaea, transmits light signals through changes in protein-protein interactions with its transducer, halobacterial transducer protein I (HtrI). Haloarchaea also have another sensor pigment, sensory rhodopsin II (SRII), which functions as a receptor regulating negative phototaxis. Compared with HsSRI, the signal relay mechanism of SRII is well characterized because SRII from Natronomonus pharaonis (NpSRII) is much more stable than HsSRI and HsSRII, especially in dilute salt solutions and is much more resistant to detergents. Two genes encoding SRI homologs were identified from the genome sequence of the eubacterium Salinibacter ruber. Those sequences are distantly related to HsSRI (∼40% identity) and contain most of the amino acid residues identified as necessary for its function. To determine whether those genes encode functional protein(s), we cloned and expressed them in Escherichia coli. One of them (SrSRI) was expressed well as a recombinant protein having all-trans retinal as a chromophore. UV-Vis, low-temperature UVVis, pH-titration, and flash photolysis experiments revealed that the photochemical properties of SrSRI are similar to those of HsSRI. In addition to the expression system, the high stability of SrSRI makes it possible to prepare large amounts of protein and enables studies of mutant proteins that will allow new approaches to investigate the photosignaling process of SRI-HtrI.

Original languageEnglish
Pages (from-to)23533-23541
Number of pages9
JournalJournal of Biological Chemistry
Volume283
Issue number35
DOIs
Publication statusPublished - Aug 29 2008
Externally publishedYes

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ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology

Cite this

Kitajima-Ihara, T., Furutani, Y., Suzuki, D., Ihara, K., Kandori, H., Homma, M., & Sudo, Y. (2008). Salinibacter sensory rhodopsin: Sensory rhodopsin I-like protein from a eubacterium. Journal of Biological Chemistry, 283(35), 23533-23541. https://doi.org/10.1074/jbc.M802990200