Salicylic acid induces a cytosolic Ca2+ elevation in yeast

Izumi C. Mori, Hidetoshi Iida, Frederick I. Tsuji, Minoru Isobe, Nobuyuki Uozumi, Shoshi Muto

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Cytosolic free calcium ion concentration ([Ca2+]cyt) after a salicylic acid (SA)-stimulus was monitored in cells of the yeast Saccharomyces cerevisiae expressing apoaequorin, which constitutes a Ca2+-sensitive luminescent protein, aequorin, when combined with coelenterazine. SA induced a transient [Ca2+]cyt elevation that was dependent on the concentration of SA and pH of the SA solution. The SA-induced [Ca2+]cyt elevation was not reduced in Ca2+-deficient medium, suggesting that Ca2+ was mobilized from an intracellular Ca2+ store(s). Benzoic acid, butyric acid and sorbic acid did not induced a [Ca2+]cyt elevation.

Original languageEnglish
Pages (from-to)986-989
Number of pages4
JournalBioscience, Biotechnology and Biochemistry
Volume62
Issue number5
DOIs
Publication statusPublished - Jan 1 1998
Externally publishedYes

Keywords

  • Aequorin
  • Cytosolic Ca
  • Saccharomyces cerevisiae
  • Salicylic acid

ASJC Scopus subject areas

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

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