Salicylic Acid Induces a Cytosolic Ca2+ Elevation in Yeast

Izumi Mori, Hidetoshi Iida, Frederick I. Tsuji, Minoru Isobe, Nobuyuki Uozumi, Shoshi Muto

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13 Citations (Scopus)

Abstract

Cytosolic free calcium ion concentration ([Ca2+]cyt) after a salicylic acid (SA)-stimulus was monitored in cells of the yeast Saccharomyces cerevisiae expressing apoaequorin, which constitutes a Ca2+-sensitive luminescent protein, aequorin, when combined with coelenterazine. SA induced a transient [Ca2+]cyt elevation that was dependent on the concentration of SA and pH of the SA solution. The SA-induced [Ca2+]cyt elevation was not reduced in Ca2+-deficient medium, suggesting that Ca2+ was mobilized from an intracellular Ca2+ store(s). Benzoic acid, butyric acid and sorbic acid did not induced a [Ca2+]cyt elevation.

Original languageEnglish
Pages (from-to)986-989
Number of pages4
JournalBioscience, Biotechnology and Biochemistry
Volume62
Issue number5
Publication statusPublished - May 1998
Externally publishedYes

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Keywords

  • Aequorin
  • Cytosolic Ca
  • Saccharomyces cerevisiae
  • Salicylic acid

ASJC Scopus subject areas

  • Food Science
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Biotechnology
  • Chemistry (miscellaneous)
  • Applied Microbiology and Biotechnology
  • Bioengineering

Cite this

Mori, I., Iida, H., Tsuji, F. I., Isobe, M., Uozumi, N., & Muto, S. (1998). Salicylic Acid Induces a Cytosolic Ca2+ Elevation in Yeast. Bioscience, Biotechnology and Biochemistry, 62(5), 986-989.