Salicylic acid-induced lipid peroxidation in rat liver microsomes

Hirokazu Doi, Yasuhiro Masubuchi, Shizuo Narimatsu, Ryuichiro Nishigaki, Toshiharu Horie

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Rat liver microsomes (1 mg protein/ml) were incubated at 37 °C with non-steroidal anti-inflammatory drag, salicylic acid (5 mM) in the presence of 0.2 mM NADPH. The amounts of thiobarbituric acid reactive-substances (TBARS) were increased remarkably by the incubation for 30min. TBARS formation was dependent on salicylic acid concentration. Salicylic acid- induced lipid peroxidation was not observed in the absence of NADPH. It was also inhibited by the presence of cytochrome P450 inhibitor, SKF-525A. The singlet oxygen's scavengers such as histidine suppressed salicylic acid- induced lipid peroxidation. These results suggested that salicylic acid- induced lipid peroxidation was coupled with the metabolism through cytochrome P450 in rat liver microsomes. The singlet oxygen may be involved in the reaction.

Original languageEnglish
Pages (from-to)265-271
Number of pages7
JournalResearch Communications in Molecular Pathology and Pharmacology
Volume100
Issue number3
Publication statusPublished - Jun 1998
Externally publishedYes

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Salicylic Acid
Liver Microsomes
Lipid Peroxidation
Singlet Oxygen
Thiobarbituric Acid Reactive Substances
NADP
Cytochrome P-450 Enzyme System
Proadifen
Histidine
Anti-Inflammatory Agents
Proteins

ASJC Scopus subject areas

  • Pharmacology, Toxicology and Pharmaceutics(all)
  • Pathology and Forensic Medicine

Cite this

Doi, H., Masubuchi, Y., Narimatsu, S., Nishigaki, R., & Horie, T. (1998). Salicylic acid-induced lipid peroxidation in rat liver microsomes. Research Communications in Molecular Pathology and Pharmacology, 100(3), 265-271.

Salicylic acid-induced lipid peroxidation in rat liver microsomes. / Doi, Hirokazu; Masubuchi, Yasuhiro; Narimatsu, Shizuo; Nishigaki, Ryuichiro; Horie, Toshiharu.

In: Research Communications in Molecular Pathology and Pharmacology, Vol. 100, No. 3, 06.1998, p. 265-271.

Research output: Contribution to journalArticle

Doi, H, Masubuchi, Y, Narimatsu, S, Nishigaki, R & Horie, T 1998, 'Salicylic acid-induced lipid peroxidation in rat liver microsomes', Research Communications in Molecular Pathology and Pharmacology, vol. 100, no. 3, pp. 265-271.
Doi, Hirokazu ; Masubuchi, Yasuhiro ; Narimatsu, Shizuo ; Nishigaki, Ryuichiro ; Horie, Toshiharu. / Salicylic acid-induced lipid peroxidation in rat liver microsomes. In: Research Communications in Molecular Pathology and Pharmacology. 1998 ; Vol. 100, No. 3. pp. 265-271.
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