TY - JOUR
T1 - Salicylic acid-induced lipid peroxidation in rat liver microsomes
AU - Doi, Hirokazu
AU - Masubuchi, Yasuhiro
AU - Narimatsu, Shizuo
AU - Nishigaki, Ryuichiro
AU - Horie, Toshiharu
N1 - Copyright:
Copyright 2007 Elsevier B.V., All rights reserved.
PY - 1998/6
Y1 - 1998/6
N2 - Rat liver microsomes (1 mg protein/ml) were incubated at 37 °C with non-steroidal anti-inflammatory drag, salicylic acid (5 mM) in the presence of 0.2 mM NADPH. The amounts of thiobarbituric acid reactive-substances (TBARS) were increased remarkably by the incubation for 30min. TBARS formation was dependent on salicylic acid concentration. Salicylic acid- induced lipid peroxidation was not observed in the absence of NADPH. It was also inhibited by the presence of cytochrome P450 inhibitor, SKF-525A. The singlet oxygen's scavengers such as histidine suppressed salicylic acid- induced lipid peroxidation. These results suggested that salicylic acid- induced lipid peroxidation was coupled with the metabolism through cytochrome P450 in rat liver microsomes. The singlet oxygen may be involved in the reaction.
AB - Rat liver microsomes (1 mg protein/ml) were incubated at 37 °C with non-steroidal anti-inflammatory drag, salicylic acid (5 mM) in the presence of 0.2 mM NADPH. The amounts of thiobarbituric acid reactive-substances (TBARS) were increased remarkably by the incubation for 30min. TBARS formation was dependent on salicylic acid concentration. Salicylic acid- induced lipid peroxidation was not observed in the absence of NADPH. It was also inhibited by the presence of cytochrome P450 inhibitor, SKF-525A. The singlet oxygen's scavengers such as histidine suppressed salicylic acid- induced lipid peroxidation. These results suggested that salicylic acid- induced lipid peroxidation was coupled with the metabolism through cytochrome P450 in rat liver microsomes. The singlet oxygen may be involved in the reaction.
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M3 - Article
C2 - 9730006
AN - SCOPUS:0031853212
SN - 1078-0297
VL - 100
SP - 265
EP - 271
JO - Research Communications in Molecular Pathology and Pharmacology
JF - Research Communications in Molecular Pathology and Pharmacology
IS - 3
ER -