Salicylic acid-, but not cytokinin-induced, resistance to WCIMV is associated with increased expression of SA-dependent resistance genes in Phaseolus vulgaris

Ivan Galis, Jennifer L. Smith, Paula E. Jameson

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Two-week-old Phaseolus vulgaris plants, wick-fed with 1 mmol/L salicylic acid (SA) or 50 nmol/L dihydrozeatin (DHZ), showed partial inhibition of the accumulation of white clover mosaic virus (WCIMV) in infected primary leaves. This inhibition was measured as a decrease in the accumulation of both viral mRNA and viral coat protein, especially at the early stages of infection. Salicylic acid treatment resulted in moderately increased expression of phenylalanine ammonia lyase (PAL), NPR1, PR1 and HSP70 genes that participate in resistance to pathogens in plants. In contrast, DHZ treatments d d not induce significant changes in expression of these genes. The expression of the P. vulgaris alternative oxidase (AOX) gene homolog, an enzyme implicated in plant resistance to viruses, showed low constitutive expression during the first 11 days post-infection and was not affected by either SA or DHZ. It appears that, while SA induced the NPR1-PR1 pathogen defense pathway genes, both SA and DHZ may use a different pathway to induce resistance to WCIMV infection in P. vulgaris plants.

Original languageEnglish
Pages (from-to)459-466
Number of pages8
JournalJournal of Plant Physiology
Volume161
Issue number4
DOIs
Publication statusPublished - 2004
Externally publishedYes

Fingerprint

Cytokinins
Phaseolus
Salicylic Acid
dihydrozeatin
induced resistance
salicylic acid
cytokinins
Phaseolus vulgaris
Genes
genes
White clover mosaic virus
Infection
infection
Phenylalanine Ammonia-Lyase
Capillary Action
Mosaic Viruses
Medicago
viral proteins
Capsid Proteins
phenylalanine ammonia-lyase

Keywords

  • Cytokinin
  • Dihydrozeatin (DHZ)
  • Phaseolus vulgaris
  • Salicylic acid (SA)
  • Virus resistance
  • White clover mosaic virus (WCIMV)

ASJC Scopus subject areas

  • Plant Science

Cite this

Salicylic acid-, but not cytokinin-induced, resistance to WCIMV is associated with increased expression of SA-dependent resistance genes in Phaseolus vulgaris. / Galis, Ivan; Smith, Jennifer L.; Jameson, Paula E.

In: Journal of Plant Physiology, Vol. 161, No. 4, 2004, p. 459-466.

Research output: Contribution to journalArticle

@article{7091509e2d4043599fed3cd7eab9c0d4,
title = "Salicylic acid-, but not cytokinin-induced, resistance to WCIMV is associated with increased expression of SA-dependent resistance genes in Phaseolus vulgaris",
abstract = "Two-week-old Phaseolus vulgaris plants, wick-fed with 1 mmol/L salicylic acid (SA) or 50 nmol/L dihydrozeatin (DHZ), showed partial inhibition of the accumulation of white clover mosaic virus (WCIMV) in infected primary leaves. This inhibition was measured as a decrease in the accumulation of both viral mRNA and viral coat protein, especially at the early stages of infection. Salicylic acid treatment resulted in moderately increased expression of phenylalanine ammonia lyase (PAL), NPR1, PR1 and HSP70 genes that participate in resistance to pathogens in plants. In contrast, DHZ treatments d d not induce significant changes in expression of these genes. The expression of the P. vulgaris alternative oxidase (AOX) gene homolog, an enzyme implicated in plant resistance to viruses, showed low constitutive expression during the first 11 days post-infection and was not affected by either SA or DHZ. It appears that, while SA induced the NPR1-PR1 pathogen defense pathway genes, both SA and DHZ may use a different pathway to induce resistance to WCIMV infection in P. vulgaris plants.",
keywords = "Cytokinin, Dihydrozeatin (DHZ), Phaseolus vulgaris, Salicylic acid (SA), Virus resistance, White clover mosaic virus (WCIMV)",
author = "Ivan Galis and Smith, {Jennifer L.} and Jameson, {Paula E.}",
year = "2004",
doi = "10.1078/0176-1617-01255",
language = "English",
volume = "161",
pages = "459--466",
journal = "Journal of Plant Physiology",
issn = "0176-1617",
publisher = "Urban und Fischer Verlag GmbH und Co. KG",
number = "4",

}

TY - JOUR

T1 - Salicylic acid-, but not cytokinin-induced, resistance to WCIMV is associated with increased expression of SA-dependent resistance genes in Phaseolus vulgaris

AU - Galis, Ivan

AU - Smith, Jennifer L.

AU - Jameson, Paula E.

PY - 2004

Y1 - 2004

N2 - Two-week-old Phaseolus vulgaris plants, wick-fed with 1 mmol/L salicylic acid (SA) or 50 nmol/L dihydrozeatin (DHZ), showed partial inhibition of the accumulation of white clover mosaic virus (WCIMV) in infected primary leaves. This inhibition was measured as a decrease in the accumulation of both viral mRNA and viral coat protein, especially at the early stages of infection. Salicylic acid treatment resulted in moderately increased expression of phenylalanine ammonia lyase (PAL), NPR1, PR1 and HSP70 genes that participate in resistance to pathogens in plants. In contrast, DHZ treatments d d not induce significant changes in expression of these genes. The expression of the P. vulgaris alternative oxidase (AOX) gene homolog, an enzyme implicated in plant resistance to viruses, showed low constitutive expression during the first 11 days post-infection and was not affected by either SA or DHZ. It appears that, while SA induced the NPR1-PR1 pathogen defense pathway genes, both SA and DHZ may use a different pathway to induce resistance to WCIMV infection in P. vulgaris plants.

AB - Two-week-old Phaseolus vulgaris plants, wick-fed with 1 mmol/L salicylic acid (SA) or 50 nmol/L dihydrozeatin (DHZ), showed partial inhibition of the accumulation of white clover mosaic virus (WCIMV) in infected primary leaves. This inhibition was measured as a decrease in the accumulation of both viral mRNA and viral coat protein, especially at the early stages of infection. Salicylic acid treatment resulted in moderately increased expression of phenylalanine ammonia lyase (PAL), NPR1, PR1 and HSP70 genes that participate in resistance to pathogens in plants. In contrast, DHZ treatments d d not induce significant changes in expression of these genes. The expression of the P. vulgaris alternative oxidase (AOX) gene homolog, an enzyme implicated in plant resistance to viruses, showed low constitutive expression during the first 11 days post-infection and was not affected by either SA or DHZ. It appears that, while SA induced the NPR1-PR1 pathogen defense pathway genes, both SA and DHZ may use a different pathway to induce resistance to WCIMV infection in P. vulgaris plants.

KW - Cytokinin

KW - Dihydrozeatin (DHZ)

KW - Phaseolus vulgaris

KW - Salicylic acid (SA)

KW - Virus resistance

KW - White clover mosaic virus (WCIMV)

UR - http://www.scopus.com/inward/record.url?scp=2342635060&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=2342635060&partnerID=8YFLogxK

U2 - 10.1078/0176-1617-01255

DO - 10.1078/0176-1617-01255

M3 - Article

C2 - 15128033

AN - SCOPUS:2342635060

VL - 161

SP - 459

EP - 466

JO - Journal of Plant Physiology

JF - Journal of Plant Physiology

SN - 0176-1617

IS - 4

ER -