Abstract
Two-week-old Phaseolus vulgaris plants, wick-fed with 1 mmol/L salicylic acid (SA) or 50 nmol/L dihydrozeatin (DHZ), showed partial inhibition of the accumulation of white clover mosaic virus (WCIMV) in infected primary leaves. This inhibition was measured as a decrease in the accumulation of both viral mRNA and viral coat protein, especially at the early stages of infection. Salicylic acid treatment resulted in moderately increased expression of phenylalanine ammonia lyase (PAL), NPR1, PR1 and HSP70 genes that participate in resistance to pathogens in plants. In contrast, DHZ treatments d d not induce significant changes in expression of these genes. The expression of the P. vulgaris alternative oxidase (AOX) gene homolog, an enzyme implicated in plant resistance to viruses, showed low constitutive expression during the first 11 days post-infection and was not affected by either SA or DHZ. It appears that, while SA induced the NPR1-PR1 pathogen defense pathway genes, both SA and DHZ may use a different pathway to induce resistance to WCIMV infection in P. vulgaris plants.
| Original language | English |
|---|---|
| Pages (from-to) | 459-466 |
| Number of pages | 8 |
| Journal | Journal of Plant Physiology |
| Volume | 161 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - 2004 |
| Externally published | Yes |
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Keywords
- Cytokinin
- Dihydrozeatin (DHZ)
- Phaseolus vulgaris
- Salicylic acid (SA)
- Virus resistance
- White clover mosaic virus (WCIMV)
ASJC Scopus subject areas
- Plant Science
Cite this
Salicylic acid-, but not cytokinin-induced, resistance to WCIMV is associated with increased expression of SA-dependent resistance genes in Phaseolus vulgaris. / Galis, Ivan; Smith, Jennifer L.; Jameson, Paula E.
In: Journal of Plant Physiology, Vol. 161, No. 4, 2004, p. 459-466.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Salicylic acid-, but not cytokinin-induced, resistance to WCIMV is associated with increased expression of SA-dependent resistance genes in Phaseolus vulgaris
AU - Galis, Ivan
AU - Smith, Jennifer L.
AU - Jameson, Paula E.
PY - 2004
Y1 - 2004
N2 - Two-week-old Phaseolus vulgaris plants, wick-fed with 1 mmol/L salicylic acid (SA) or 50 nmol/L dihydrozeatin (DHZ), showed partial inhibition of the accumulation of white clover mosaic virus (WCIMV) in infected primary leaves. This inhibition was measured as a decrease in the accumulation of both viral mRNA and viral coat protein, especially at the early stages of infection. Salicylic acid treatment resulted in moderately increased expression of phenylalanine ammonia lyase (PAL), NPR1, PR1 and HSP70 genes that participate in resistance to pathogens in plants. In contrast, DHZ treatments d d not induce significant changes in expression of these genes. The expression of the P. vulgaris alternative oxidase (AOX) gene homolog, an enzyme implicated in plant resistance to viruses, showed low constitutive expression during the first 11 days post-infection and was not affected by either SA or DHZ. It appears that, while SA induced the NPR1-PR1 pathogen defense pathway genes, both SA and DHZ may use a different pathway to induce resistance to WCIMV infection in P. vulgaris plants.
AB - Two-week-old Phaseolus vulgaris plants, wick-fed with 1 mmol/L salicylic acid (SA) or 50 nmol/L dihydrozeatin (DHZ), showed partial inhibition of the accumulation of white clover mosaic virus (WCIMV) in infected primary leaves. This inhibition was measured as a decrease in the accumulation of both viral mRNA and viral coat protein, especially at the early stages of infection. Salicylic acid treatment resulted in moderately increased expression of phenylalanine ammonia lyase (PAL), NPR1, PR1 and HSP70 genes that participate in resistance to pathogens in plants. In contrast, DHZ treatments d d not induce significant changes in expression of these genes. The expression of the P. vulgaris alternative oxidase (AOX) gene homolog, an enzyme implicated in plant resistance to viruses, showed low constitutive expression during the first 11 days post-infection and was not affected by either SA or DHZ. It appears that, while SA induced the NPR1-PR1 pathogen defense pathway genes, both SA and DHZ may use a different pathway to induce resistance to WCIMV infection in P. vulgaris plants.
KW - Cytokinin
KW - Dihydrozeatin (DHZ)
KW - Phaseolus vulgaris
KW - Salicylic acid (SA)
KW - Virus resistance
KW - White clover mosaic virus (WCIMV)
UR - http://www.scopus.com/inward/record.url?scp=2342635060&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=2342635060&partnerID=8YFLogxK
U2 - 10.1078/0176-1617-01255
DO - 10.1078/0176-1617-01255
M3 - Article
C2 - 15128033
AN - SCOPUS:2342635060
VL - 161
SP - 459
EP - 466
JO - Journal of Plant Physiology
JF - Journal of Plant Physiology
SN - 0176-1617
IS - 4
ER -