SA channel mediates superoxide production in HUVECs

Kiyoshi Aikawa, Naomichi Nishikimi, Tsunehisa Sakurai, Yuji Nimura, Masahiro Sokabe, Keiji Naruse

Research output: Contribution to journalArticlepeer-review

16 Citations (Scopus)

Abstract

Superoxide production in response to cyclic stretch (1 Hz, 20 % in length) was investigated in human umbilical vein endothelial cells (HUVECs). The basal production of superoxide without stretch increased gradually, while the production of superoxide with stretch increased significantly as compared to that without stretch and it became significant 80 min after the onset of cyclic stretch (P<0.05, n=8-14). The superoxide production increased in a stretch-dependent manner and became significant when stretch was more than 10 % (p<0.05, n=11-16). To investigate the involvement of SA channel, we added Gd3+ or EGTA in the reaction solution and examined the stretch-induced superoxide production. In cells stretched in the presence of 20μM Gd3+, the stretch-induced superoxide production was significantly inhibited (at 120 min, p<0.05, n=8-18). The cyclic stretch-induced superoxide production was also significantly inhibited by the removal of extracellular Ca2+ with 5 mM EGTA (at 120 min, p<0.05, n=8-18). Neither the application of Gd3+ nor the removal of extracellular Ca2+ significantly changed the basal production of superoxide. These data suggest that the stretch-induced superoxide production increases in time- and stretch-dependent manner and that the stretch-induced superoxide production in HUVECs is regulated by Ca2+ influx through SA channels.

Original languageEnglish
Pages (from-to)1717-1724
Number of pages8
JournalLife Sciences
Volume69
Issue number15
DOIs
Publication statusPublished - Aug 31 2001
Externally publishedYes

Keywords

  • Ca
  • Gd
  • Stretch-activated channel

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Pharmacology, Toxicology and Pharmaceutics(all)

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