SA channel mediates superoxide production in HUVECs

Kiyoshi Aikawa, Naomichi Nishikimi, Tsunehisa Sakurai, Yuji Nimura, Masahiro Sokabe, Keiji Naruse

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Superoxide production in response to cyclic stretch (1 Hz, 20 % in length) was investigated in human umbilical vein endothelial cells (HUVECs). The basal production of superoxide without stretch increased gradually, while the production of superoxide with stretch increased significantly as compared to that without stretch and it became significant 80 min after the onset of cyclic stretch (P3+ or EGTA in the reaction solution and examined the stretch-induced superoxide production. In cells stretched in the presence of 20μM Gd3+, the stretch-induced superoxide production was significantly inhibited (at 120 min, p2+ with 5 mM EGTA (at 120 min, p3+ nor the removal of extracellular Ca2+ significantly changed the basal production of superoxide. These data suggest that the stretch-induced superoxide production increases in time- and stretch-dependent manner and that the stretch-induced superoxide production in HUVECs is regulated by Ca2+ influx through SA channels.

Original languageEnglish
Pages (from-to)1717-1724
Number of pages8
JournalLife Sciences
Volume69
Issue number15
DOIs
Publication statusPublished - Aug 31 2001
Externally publishedYes

Fingerprint

Endothelial cells
Human Umbilical Vein Endothelial Cells
Superoxides
Egtazic Acid

Keywords

  • Ca
  • Gd
  • Stretch-activated channel

ASJC Scopus subject areas

  • Pharmacology

Cite this

Aikawa, K., Nishikimi, N., Sakurai, T., Nimura, Y., Sokabe, M., & Naruse, K. (2001). SA channel mediates superoxide production in HUVECs. Life Sciences, 69(15), 1717-1724. https://doi.org/10.1016/S0024-3205(01)01256-5

SA channel mediates superoxide production in HUVECs. / Aikawa, Kiyoshi; Nishikimi, Naomichi; Sakurai, Tsunehisa; Nimura, Yuji; Sokabe, Masahiro; Naruse, Keiji.

In: Life Sciences, Vol. 69, No. 15, 31.08.2001, p. 1717-1724.

Research output: Contribution to journalArticle

Aikawa, K, Nishikimi, N, Sakurai, T, Nimura, Y, Sokabe, M & Naruse, K 2001, 'SA channel mediates superoxide production in HUVECs', Life Sciences, vol. 69, no. 15, pp. 1717-1724. https://doi.org/10.1016/S0024-3205(01)01256-5
Aikawa K, Nishikimi N, Sakurai T, Nimura Y, Sokabe M, Naruse K. SA channel mediates superoxide production in HUVECs. Life Sciences. 2001 Aug 31;69(15):1717-1724. https://doi.org/10.1016/S0024-3205(01)01256-5
Aikawa, Kiyoshi ; Nishikimi, Naomichi ; Sakurai, Tsunehisa ; Nimura, Yuji ; Sokabe, Masahiro ; Naruse, Keiji. / SA channel mediates superoxide production in HUVECs. In: Life Sciences. 2001 ; Vol. 69, No. 15. pp. 1717-1724.
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