S100A7 promotes the migration and invasion of osteosarcoma cells via the receptor for advanced glycation end products

Ken Kataoka, Tomoyuki Ono, Hitoshi Murata, Mika Morishita, Ken-ichi Yamamoto, Masakiyo Sakaguchi, Nam Ho Huh

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Osteosarcoma is the most common malignant tumor of bone in childhood and adolescence. Despite intensive research for new therapies, the outcome in patients with metastasis remains extremely poor. S100 proteins are involved in the proliferation, cell cycle progression and metastasis of numerous malignant tumors, including osteosarcoma. In the present study, we identified S100A7 as a candidate to promote the migration of osteosarcoma cells. S100A7 promoted the migration and invasion of osteosarcoma cells as assayed in vitro. An in vitro pull-down assay revealed the binding of the recombinant S100A7 protein with its putative receptor, the receptor for advanced glycation end products (RAGE). The downregulation of RAGE by a specific siRNA markedly suppressed the migration and invasion of osteosarcoma cells. Furthermore, the matrix metalloproteinase activity of osteosarcoma cells was enhanced by S100A7 and suppressed by the downregulation of RAGE. These results indicate that S100A7 promotes the migration and invasion of osteosarcoma cells through RAGE. The S100A7-RAGE axis may thus be a new target for preventing the invasion and/or metastasis of osteosarcoma.

Original languageEnglish
Pages (from-to)1149-1153
Number of pages5
JournalOncology Letters
Volume3
Issue number5
DOIs
Publication statusPublished - May 2012

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Osteosarcoma
Neoplasm Metastasis
Down-Regulation
S100 Proteins
Advanced Glycosylation End Product-Specific Receptor
Matrix Metalloproteinases
Recombinant Proteins
Small Interfering RNA
Cell Movement
Cell Cycle
Bone and Bones
Research

Keywords

  • Invasion
  • Migration
  • Osteosarcoma
  • RAGE
  • S100A7

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

S100A7 promotes the migration and invasion of osteosarcoma cells via the receptor for advanced glycation end products. / Kataoka, Ken; Ono, Tomoyuki; Murata, Hitoshi; Morishita, Mika; Yamamoto, Ken-ichi; Sakaguchi, Masakiyo; Huh, Nam Ho.

In: Oncology Letters, Vol. 3, No. 5, 05.2012, p. 1149-1153.

Research output: Contribution to journalArticle

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