S100A1 Is a Novel Molecular Chaperone and a Member of the Hsp70/Hsp90 Multichaperone Complex

Miki Okada, Takashi Hatakeyama, Hideaki Itoh, Naoki Tokuta, Hiroshi Tokumitsu, Ryoji Kobayashi

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

Although calmodulin is known to be a component of the Hsp70/Hsp90 multichaperone complex, the functional role of the protein remains uncertain. In this study, we have identified S100A1, but not calmodulin or other S100 proteins, as a potent molecular chaperone and a new member of the multichaperone complex. Glutathione S-transferase pull-down assays and co-immunoprecipitation experiments indicated the formation of stable complexes between S100A1 and Hsp90, Hsp70, FKBP52, and CyP40 both in vitro and in mammalian cells. S100A1 potently protected citrate synthase, aldolase, glyceraldehyde-3-phosphate dehydrogenase, and rhodanese from heat-induced aggregation and suppressed the aggregation of chemically denatured rhodanese and citrate synthase during the refolding pathway. In addition, S100A1 suppressed the heat-induced inactivation of citrate synthase activity, similar to that for Hsp90 and p23. The chaperone activity of S100A1 was antagonized by calmodulin antagonists, such as fluphenazine and prenylamine, that is, indeed an intrinsic function of the protein. The overexpression of S100A1 in COS-7 cells protected transiently expressed firefly luciferase and Escherichia coli β-galactosidase from inactivation during heat shock. The results demonstrate a novel physiological function for S100A1 and bring us closer to a comprehensive understanding of the molecular mechanisms of the Hsp70/Hsp90 multichaperone complex.

Original languageEnglish
Pages (from-to)4221-4233
Number of pages13
JournalJournal of Biological Chemistry
Volume279
Issue number6
DOIs
Publication statusPublished - Feb 6 2004
Externally publishedYes

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Citrate (si)-Synthase
Molecular Chaperones
Calmodulin
Thiosulfate Sulfurtransferase
Hot Temperature
Prenylamine
Agglomeration
Galactosidases
Fluphenazine
Firefly Luciferases
Fructose-Bisphosphate Aldolase
Glyceraldehyde-3-Phosphate Dehydrogenases
S100 Proteins
COS Cells
Glutathione Transferase
Immunoprecipitation
Escherichia coli
Shock
Assays
Proteins

ASJC Scopus subject areas

  • Biochemistry

Cite this

S100A1 Is a Novel Molecular Chaperone and a Member of the Hsp70/Hsp90 Multichaperone Complex. / Okada, Miki; Hatakeyama, Takashi; Itoh, Hideaki; Tokuta, Naoki; Tokumitsu, Hiroshi; Kobayashi, Ryoji.

In: Journal of Biological Chemistry, Vol. 279, No. 6, 06.02.2004, p. 4221-4233.

Research output: Contribution to journalArticle

Okada, Miki ; Hatakeyama, Takashi ; Itoh, Hideaki ; Tokuta, Naoki ; Tokumitsu, Hiroshi ; Kobayashi, Ryoji. / S100A1 Is a Novel Molecular Chaperone and a Member of the Hsp70/Hsp90 Multichaperone Complex. In: Journal of Biological Chemistry. 2004 ; Vol. 279, No. 6. pp. 4221-4233.
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