S100 protein-immunoreactive trigeminal neurons innervating the rat molar tooth pulp

Hiroyuki Ichikawa, Yi Fen He, Tomosada Sugimoto

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

S100-immunoreactivity (ir) was examined in tooth pulp primary neurons of the rat. An immunofluorescence method demonstrated that the molar tooth pulp contained S100-immunoreactive (ir) nerve fibers. In the root pulp, pulp horn and roof of the pulp chamber, S100-ir smooth and varicose fibers ramified and formed subodontoblastic nerve plexuses. All the fibers became varicose at the base of the odontoblastic layer and extended to the odontoblastic layer. Some varicose endings could be traced into the dentin. The trigeminal neurons retrogradely labeled with fluorogold (FG) from the first and second maxillary molar tooth pulps exhibited S100- and parvalbumin-ir. Approximately 60% and 24% of the labeled cells were ir for S100 and parvalbumin, respectively. Virtually all parvalbumin-ir FG-labeled cells showed S100-ir, while 40% of S100-ir ones coexpressed parvalbumin-ir. An immunoelectron microscopic method revealed that all myelinated axons and half of the unmyelinated axons in the root pulp contained S100-ir. In the odontoblastic layer, predentin and dentin, S100-ir neurites lost the Schwann cell ensheathment and made close contact with cell bodies and processes of odontoblasts. The odontoblastic layer also contained parvalbumin-ir neurites. These neurites were devoid of the Schwann cell ensheathment and in close apposition to cell bodies and processes of odontoblasts. S100-ir pulpal axons seemed to be insensitive to repeated neonatal capsaicin treatment. This study suggests that S100-ir tooth pulp primary neurons are mostly myelinated and that S100-ir unmyelinated axons in the root pulp are preterminal segments of myelinated stem axons.

Original languageEnglish
Pages (from-to)128-133
Number of pages6
JournalSomatosensory and Motor Research
Volume15
Issue number2
DOIs
Publication statusPublished - 1998

Fingerprint

Parvalbumins
S100 Proteins
Axons
Tooth
Neurons
Neurites
Odontoblasts
Deciduous Tooth
Schwann Cells
Dentin
Dental Pulp Cavity
Capsaicin
Horns
Nerve Fibers
Fluorescent Antibody Technique

Keywords

  • Capsaicin
  • Parvalbumin
  • Rat
  • S100
  • Tooth pulp
  • Trigeminal ganglion

ASJC Scopus subject areas

  • Physiology
  • Neuroscience(all)

Cite this

S100 protein-immunoreactive trigeminal neurons innervating the rat molar tooth pulp. / Ichikawa, Hiroyuki; He, Yi Fen; Sugimoto, Tomosada.

In: Somatosensory and Motor Research, Vol. 15, No. 2, 1998, p. 128-133.

Research output: Contribution to journalArticle

Ichikawa, Hiroyuki ; He, Yi Fen ; Sugimoto, Tomosada. / S100 protein-immunoreactive trigeminal neurons innervating the rat molar tooth pulp. In: Somatosensory and Motor Research. 1998 ; Vol. 15, No. 2. pp. 128-133.
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