S100 protein-immunoreactive primary sensory neurons in the trigeminal and dorsal root ganglia of the rat

Hiroyuki Ichikawa; David M. Jacobowitz; Tomosada Sugimoto

doi:10.1016/S0006-8993(96)01364-9

S100 protein-immunoreactive primary sensory neurons in the trigeminal and dorsal root ganglia of the rat

Hiroyuki Ichikawa, David M. Jacobowitz, Tomosada Sugimoto

Research output: Contribution to journal › Article

47 Citations (Scopus)

Abstract

The cell body size (cross-sectional area) of S100-immunoreactive (-ir) primary neurons was measured in the trigeminal (TG) and lumbar dorsal root ganglia (DRG). About half of the neurons exhibited S100-immunoreactivity (-ir) in the DRG (44.0%) and TG (59.0%). DRG neurons with cell bodies > 1200 μm² mostly exhibited S100-ir (96.5%), whereas S100-ir DRG neurons <600 μm² were rare (8.0%). 36.6% of DRG neurons in the cell size range 600-1200 μm² showed the ir. TG neurons > 800 μm² mostly exhibited S100-ir (93.1%), whereas those <400 μm² were devoid of it (positive cells 10.5%). 58.3% of TG cells in the range 400-800 μm² contained S100-ir. Double-immunofluorescence method revealed the co-expression of S100 and other calcium-binding proteins. Parvalbumin-ir neurons mostly exhibited S100-ir in the DRG (97.4%) and TG (97.0%). The co-expression of S100 and calbindin D-28k was very rare in the DRG, because the DRG contained few calbindin D-28k-ir neurons. Unlike in the DRG, numerous neurons co-expressed S100- and calbindin D-28k-ir in the TG. Most calbindin D-28k-ir TG neurons were also immunoreactive for S100 (90.7%). Sub-populations of calretinin (CR)-ir neurons co-expressed S100-ir in both the DRG (68%) and TG (50.0%). Virtually all CR-ir neurons > 1400 μm² co-expressed S100-ir in the DRG (100%) and TG (95.9%). CR-ir neurons <800 μm² were rarely exhibited S100-ir (DRG 18.0%, TG 21.9%). 71.3 and 60.5% of CR-ir neurons in the range 800-1400 μm² co-expressed S100-ir in the DRG and TG, respectively. The present study indicates that S100 is closely correlated to the primary neuronal cell size in the DRG and TG.

Original language	English
Pages (from-to)	253-257
Number of pages	5
Journal	Brain Research
Volume	748
Issue number	1-2
DOIs	https://doi.org/10.1016/S0006-8993(96)01364-9
Publication status	Published - Feb 14 1997

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S100 Proteins

Spinal Ganglia

Sensory Receptor Cells

Neurons

Cell Size

Body Size

Keywords

calbindin D-28k
calretinin
dorsal root ganglion
parvalbumin
S100
trigeminal ganglion

ASJC Scopus subject areas

Neuroscience(all)

Cite this

Ichikawa, H., Jacobowitz, D. M., & Sugimoto, T. (1997). S100 protein-immunoreactive primary sensory neurons in the trigeminal and dorsal root ganglia of the rat. Brain Research, 748(1-2), 253-257. https://doi.org/10.1016/S0006-8993(96)01364-9

S100 protein-immunoreactive primary sensory neurons in the trigeminal and dorsal root ganglia of the rat. / Ichikawa, Hiroyuki; Jacobowitz, David M.; Sugimoto, Tomosada.

In: Brain Research, Vol. 748, No. 1-2, 14.02.1997, p. 253-257.

Research output: Contribution to journal › Article

Ichikawa, H, Jacobowitz, DM & Sugimoto, T 1997, 'S100 protein-immunoreactive primary sensory neurons in the trigeminal and dorsal root ganglia of the rat', Brain Research, vol. 748, no. 1-2, pp. 253-257. https://doi.org/10.1016/S0006-8993(96)01364-9

Ichikawa H, Jacobowitz DM, Sugimoto T. S100 protein-immunoreactive primary sensory neurons in the trigeminal and dorsal root ganglia of the rat. Brain Research. 1997 Feb 14;748(1-2):253-257. https://doi.org/10.1016/S0006-8993(96)01364-9

Ichikawa, Hiroyuki ; Jacobowitz, David M. ; Sugimoto, Tomosada. / S100 protein-immunoreactive primary sensory neurons in the trigeminal and dorsal root ganglia of the rat. In: Brain Research. 1997 ; Vol. 748, No. 1-2. pp. 253-257.

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abstract = "The cell body size (cross-sectional area) of S100-immunoreactive (-ir) primary neurons was measured in the trigeminal (TG) and lumbar dorsal root ganglia (DRG). About half of the neurons exhibited S100-immunoreactivity (-ir) in the DRG (44.0{\%}) and TG (59.0{\%}). DRG neurons with cell bodies > 1200 μm2 mostly exhibited S100-ir (96.5{\%}), whereas S100-ir DRG neurons <600 μm2 were rare (8.0{\%}). 36.6{\%} of DRG neurons in the cell size range 600-1200 μm2 showed the ir. TG neurons > 800 μm2 mostly exhibited S100-ir (93.1{\%}), whereas those <400 μm2 were devoid of it (positive cells 10.5{\%}). 58.3{\%} of TG cells in the range 400-800 μm2 contained S100-ir. Double-immunofluorescence method revealed the co-expression of S100 and other calcium-binding proteins. Parvalbumin-ir neurons mostly exhibited S100-ir in the DRG (97.4{\%}) and TG (97.0{\%}). The co-expression of S100 and calbindin D-28k was very rare in the DRG, because the DRG contained few calbindin D-28k-ir neurons. Unlike in the DRG, numerous neurons co-expressed S100- and calbindin D-28k-ir in the TG. Most calbindin D-28k-ir TG neurons were also immunoreactive for S100 (90.7{\%}). Sub-populations of calretinin (CR)-ir neurons co-expressed S100-ir in both the DRG (68{\%}) and TG (50.0{\%}). Virtually all CR-ir neurons > 1400 μm2 co-expressed S100-ir in the DRG (100{\%}) and TG (95.9{\%}). CR-ir neurons <800 μm2 were rarely exhibited S100-ir (DRG 18.0{\%}, TG 21.9{\%}). 71.3 and 60.5{\%} of CR-ir neurons in the range 800-1400 μm2 co-expressed S100-ir in the DRG and TG, respectively. The present study indicates that S100 is closely correlated to the primary neuronal cell size in the DRG and TG.",

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N2 - The cell body size (cross-sectional area) of S100-immunoreactive (-ir) primary neurons was measured in the trigeminal (TG) and lumbar dorsal root ganglia (DRG). About half of the neurons exhibited S100-immunoreactivity (-ir) in the DRG (44.0%) and TG (59.0%). DRG neurons with cell bodies > 1200 μm2 mostly exhibited S100-ir (96.5%), whereas S100-ir DRG neurons <600 μm2 were rare (8.0%). 36.6% of DRG neurons in the cell size range 600-1200 μm2 showed the ir. TG neurons > 800 μm2 mostly exhibited S100-ir (93.1%), whereas those <400 μm2 were devoid of it (positive cells 10.5%). 58.3% of TG cells in the range 400-800 μm2 contained S100-ir. Double-immunofluorescence method revealed the co-expression of S100 and other calcium-binding proteins. Parvalbumin-ir neurons mostly exhibited S100-ir in the DRG (97.4%) and TG (97.0%). The co-expression of S100 and calbindin D-28k was very rare in the DRG, because the DRG contained few calbindin D-28k-ir neurons. Unlike in the DRG, numerous neurons co-expressed S100- and calbindin D-28k-ir in the TG. Most calbindin D-28k-ir TG neurons were also immunoreactive for S100 (90.7%). Sub-populations of calretinin (CR)-ir neurons co-expressed S100-ir in both the DRG (68%) and TG (50.0%). Virtually all CR-ir neurons > 1400 μm2 co-expressed S100-ir in the DRG (100%) and TG (95.9%). CR-ir neurons <800 μm2 were rarely exhibited S100-ir (DRG 18.0%, TG 21.9%). 71.3 and 60.5% of CR-ir neurons in the range 800-1400 μm2 co-expressed S100-ir in the DRG and TG, respectively. The present study indicates that S100 is closely correlated to the primary neuronal cell size in the DRG and TG.

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