TY - JOUR
T1 - S100 protein-immunoreactive primary sensory neurons in the trigeminal and dorsal root ganglia of the rat
AU - Ichikawa, Hiroyuki
AU - Jacobowitz, David M.
AU - Sugimoto, Tomosada
N1 - Copyright:
Copyright 2007 Elsevier B.V., All rights reserved.
PY - 1997/2/14
Y1 - 1997/2/14
N2 - The cell body size (cross-sectional area) of S100-immunoreactive (-ir) primary neurons was measured in the trigeminal (TG) and lumbar dorsal root ganglia (DRG). About half of the neurons exhibited S100-immunoreactivity (-ir) in the DRG (44.0%) and TG (59.0%). DRG neurons with cell bodies > 1200 μm2 mostly exhibited S100-ir (96.5%), whereas S100-ir DRG neurons < 600 μm2 were rare (8.0%). 36.6% of DRG neurons in the cell size range 600-1200 μm2 showed the ir. TG neurons > 800 μm2 mostly exhibited S100-ir (93.1%), whereas those < 400 μm2 were devoid of it (positive cells 10.5%). 58.3% of TG cells in the range 400-800 μm2 contained S100-ir. Double-immunofluorescence method revealed the co-expression of S100 and other calcium-binding proteins. Parvalbumin-ir neurons mostly exhibited S100-ir in the DRG (97.4%) and TG (97.0%). The co-expression of S100 and calbindin D-28k was very rare in the DRG, because the DRG contained few calbindin D-28k-ir neurons. Unlike in the DRG, numerous neurons co-expressed S100- and calbindin D-28k-ir in the TG. Most calbindin D-28k-ir TG neurons were also immunoreactive for S100 (90.7%). Sub-populations of calretinin (CR)-ir neurons co-expressed S100-ir in both the DRG (68%) and TG (50.0%). Virtually all CR-ir neurons > 1400 μm2 co-expressed S100-ir in the DRG (100%) and TG (95.9%). CR-ir neurons < 800 μm2 were rarely exhibited S100-ir (DRG 18.0%, TG 21.9%). 71.3 and 60.5% of CR-ir neurons in the range 800-1400 μm2 co-expressed S100-ir in the DRG and TG, respectively. The present study indicates that S100 is closely correlated to the primary neuronal cell size in the DRG and TG.
AB - The cell body size (cross-sectional area) of S100-immunoreactive (-ir) primary neurons was measured in the trigeminal (TG) and lumbar dorsal root ganglia (DRG). About half of the neurons exhibited S100-immunoreactivity (-ir) in the DRG (44.0%) and TG (59.0%). DRG neurons with cell bodies > 1200 μm2 mostly exhibited S100-ir (96.5%), whereas S100-ir DRG neurons < 600 μm2 were rare (8.0%). 36.6% of DRG neurons in the cell size range 600-1200 μm2 showed the ir. TG neurons > 800 μm2 mostly exhibited S100-ir (93.1%), whereas those < 400 μm2 were devoid of it (positive cells 10.5%). 58.3% of TG cells in the range 400-800 μm2 contained S100-ir. Double-immunofluorescence method revealed the co-expression of S100 and other calcium-binding proteins. Parvalbumin-ir neurons mostly exhibited S100-ir in the DRG (97.4%) and TG (97.0%). The co-expression of S100 and calbindin D-28k was very rare in the DRG, because the DRG contained few calbindin D-28k-ir neurons. Unlike in the DRG, numerous neurons co-expressed S100- and calbindin D-28k-ir in the TG. Most calbindin D-28k-ir TG neurons were also immunoreactive for S100 (90.7%). Sub-populations of calretinin (CR)-ir neurons co-expressed S100-ir in both the DRG (68%) and TG (50.0%). Virtually all CR-ir neurons > 1400 μm2 co-expressed S100-ir in the DRG (100%) and TG (95.9%). CR-ir neurons < 800 μm2 were rarely exhibited S100-ir (DRG 18.0%, TG 21.9%). 71.3 and 60.5% of CR-ir neurons in the range 800-1400 μm2 co-expressed S100-ir in the DRG and TG, respectively. The present study indicates that S100 is closely correlated to the primary neuronal cell size in the DRG and TG.
KW - S100
KW - calbindin D-28k
KW - calretinin
KW - dorsal root ganglion
KW - parvalbumin
KW - trigeminal ganglion
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U2 - 10.1016/S0006-8993(96)01364-9
DO - 10.1016/S0006-8993(96)01364-9
M3 - Article
C2 - 9067472
AN - SCOPUS:0031045168
VL - 748
SP - 253
EP - 257
JO - Molecular Brain Research
JF - Molecular Brain Research
SN - 0006-8993
IS - 1-2
ER -