Abstract
Although recent findings suggest that the F-box genes SFB/SLF control pollen-part S specificity in the S-RNase-based gametophytic self-incompatibility (GSI) system, how these genes operate in the system is unknown, and functional variation of pollen S genes in different species has been reported. Here, we analyzed the S locus of two species of Maloideae: apple (Malus domestica) and Japanese pear (Pyrus pyrifolia). The sequencing of a 317-kb region of the apple S9 haplotype revealed two similar F-box genes. Homologous sequences were isolated from different haplotypes of apple and Japanese pear, and they were found to be polymorphic genes derived from the S locus. Since each S haplotype contains two or three related genes, the genes were named SFBB for S locus F-box brothers. The SFBB genes are specifically expressed in pollen, and variable regions of the SFBB genes are under positive selection. In a style-specific mutant S haplotype of Japanese pear, the SFBB genes are retained. Apart from their multiplicity, SFBB genes meet the expected characteristics of pollen S. The unique multiplicity of SFBB genes as the pollen S candidate is discussed in the context of mechanistic variation in the S-RNase-based GSI system.
| Original language | English |
|---|---|
| Pages (from-to) | 1869-1881 |
| Number of pages | 13 |
| Journal | Genetics |
| Volume | 175 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - Apr 2007 |
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ASJC Scopus subject areas
- Genetics
- Genetics(clinical)
Cite this
S locus F-box brothers : Multiple and pollen-specific F-box genes with S haplotype-specific polymorphisms in apple and Japanese pear. / Sassa, Hidenori; Kakui, Hiroyuki; Miyamoto, Mayu; Suzuki, Yusuke; Hanada, Toshio; Ushijima, Koichiro; Kusaba, Makoto; Hirano, Hisashi; Koba, Takato.
In: Genetics, Vol. 175, No. 4, 04.2007, p. 1869-1881.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - S locus F-box brothers
T2 - Multiple and pollen-specific F-box genes with S haplotype-specific polymorphisms in apple and Japanese pear
AU - Sassa, Hidenori
AU - Kakui, Hiroyuki
AU - Miyamoto, Mayu
AU - Suzuki, Yusuke
AU - Hanada, Toshio
AU - Ushijima, Koichiro
AU - Kusaba, Makoto
AU - Hirano, Hisashi
AU - Koba, Takato
PY - 2007/4
Y1 - 2007/4
N2 - Although recent findings suggest that the F-box genes SFB/SLF control pollen-part S specificity in the S-RNase-based gametophytic self-incompatibility (GSI) system, how these genes operate in the system is unknown, and functional variation of pollen S genes in different species has been reported. Here, we analyzed the S locus of two species of Maloideae: apple (Malus domestica) and Japanese pear (Pyrus pyrifolia). The sequencing of a 317-kb region of the apple S9 haplotype revealed two similar F-box genes. Homologous sequences were isolated from different haplotypes of apple and Japanese pear, and they were found to be polymorphic genes derived from the S locus. Since each S haplotype contains two or three related genes, the genes were named SFBB for S locus F-box brothers. The SFBB genes are specifically expressed in pollen, and variable regions of the SFBB genes are under positive selection. In a style-specific mutant S haplotype of Japanese pear, the SFBB genes are retained. Apart from their multiplicity, SFBB genes meet the expected characteristics of pollen S. The unique multiplicity of SFBB genes as the pollen S candidate is discussed in the context of mechanistic variation in the S-RNase-based GSI system.
AB - Although recent findings suggest that the F-box genes SFB/SLF control pollen-part S specificity in the S-RNase-based gametophytic self-incompatibility (GSI) system, how these genes operate in the system is unknown, and functional variation of pollen S genes in different species has been reported. Here, we analyzed the S locus of two species of Maloideae: apple (Malus domestica) and Japanese pear (Pyrus pyrifolia). The sequencing of a 317-kb region of the apple S9 haplotype revealed two similar F-box genes. Homologous sequences were isolated from different haplotypes of apple and Japanese pear, and they were found to be polymorphic genes derived from the S locus. Since each S haplotype contains two or three related genes, the genes were named SFBB for S locus F-box brothers. The SFBB genes are specifically expressed in pollen, and variable regions of the SFBB genes are under positive selection. In a style-specific mutant S haplotype of Japanese pear, the SFBB genes are retained. Apart from their multiplicity, SFBB genes meet the expected characteristics of pollen S. The unique multiplicity of SFBB genes as the pollen S candidate is discussed in the context of mechanistic variation in the S-RNase-based GSI system.
UR - http://www.scopus.com/inward/record.url?scp=34249088085&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=34249088085&partnerID=8YFLogxK
U2 - 10.1534/genetics.106.068858
DO - 10.1534/genetics.106.068858
M3 - Article
C2 - 17237509
AN - SCOPUS:34249088085
VL - 175
SP - 1869
EP - 1881
JO - Genetics
JF - Genetics
SN - 0016-6731
IS - 4
ER -