Runx/Cbfb signaling regulates postnatal development of granular convoluted tubule in the mouse submandibular gland

Md Nurul Islam, Shinsuke Itoh, Takeshi Yanagita, Kumi Sumiyoshi, Satoru Hayano, Koh Ichi Kuremoto, Hiroshi Kurosaka, Tadashi Honjo, Noriaki Kawanabe, Hiroshi Kamioka, Takayoshi Sakai, Naozumi Ishimaru, Ichiro Taniuchi, Takashi Yamashiro

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The rodent salivary gland is not fully developed at birth and the cellular definitive differentiation takes place postnatally. However, little is known about its molecular mechanism. Results: Here we provide the loss-of-function genetic evidence that Runx signaling affects postnatal development of the submandibular gland (SMG). Core binding factor β (Cbfb) is a cotranscription factor which forms a heterodimer with Runx proteins. Cbfb was specifically expressed in the duct epithelium, specifically in the SMG. Epithelial Cbfb deficiency resulted in decrease in the size of the SMG and in the saliva secretion on postnatal day 35. The Cbfb mutant SMG specifically exhibited involution of the granular convoluted tubules (GCT), with a down-regulated expression of its marker genes, such as Klk1, Ngf, and Egf. The induction of GCT is under the control of androgens, and the Cbfb mutant SMG demonstrated down-regulated expression of Crisp3, an androgen-dependent transcript. Because the circulating testosterone or tissue dihydrotestosterone levels were not affected in the Cbfb mutants, it appears that Runx/Cbfb signaling regulate androgen receptor pathway, but does not affect the circulating testosterone levels or the enzymatic conversion to DHT. Conclusions: Runx signaling is important in the postnatal development of androgen-dependent GCT in the SMG.

Original languageEnglish
Pages (from-to)488-496
Number of pages9
JournalDevelopmental Dynamics
Volume244
Issue number3
DOIs
Publication statusPublished - Mar 1 2015

Fingerprint

Submandibular Gland
Androgens
Testosterone
Core Binding Factor alpha Subunits
Core Binding Factors
Dihydrotestosterone
Androgen Receptors
Salivary Glands
Saliva
Rodentia
Epithelium
Parturition
Genes

Keywords

  • Cbfb
  • Craniofacial
  • Gland
  • Organogenesis

ASJC Scopus subject areas

  • Developmental Biology
  • Medicine(all)

Cite this

Runx/Cbfb signaling regulates postnatal development of granular convoluted tubule in the mouse submandibular gland. / Islam, Md Nurul; Itoh, Shinsuke; Yanagita, Takeshi; Sumiyoshi, Kumi; Hayano, Satoru; Kuremoto, Koh Ichi; Kurosaka, Hiroshi; Honjo, Tadashi; Kawanabe, Noriaki; Kamioka, Hiroshi; Sakai, Takayoshi; Ishimaru, Naozumi; Taniuchi, Ichiro; Yamashiro, Takashi.

In: Developmental Dynamics, Vol. 244, No. 3, 01.03.2015, p. 488-496.

Research output: Contribution to journalArticle

Islam, MN, Itoh, S, Yanagita, T, Sumiyoshi, K, Hayano, S, Kuremoto, KI, Kurosaka, H, Honjo, T, Kawanabe, N, Kamioka, H, Sakai, T, Ishimaru, N, Taniuchi, I & Yamashiro, T 2015, 'Runx/Cbfb signaling regulates postnatal development of granular convoluted tubule in the mouse submandibular gland', Developmental Dynamics, vol. 244, no. 3, pp. 488-496. https://doi.org/10.1002/dvdy.24231
Islam, Md Nurul ; Itoh, Shinsuke ; Yanagita, Takeshi ; Sumiyoshi, Kumi ; Hayano, Satoru ; Kuremoto, Koh Ichi ; Kurosaka, Hiroshi ; Honjo, Tadashi ; Kawanabe, Noriaki ; Kamioka, Hiroshi ; Sakai, Takayoshi ; Ishimaru, Naozumi ; Taniuchi, Ichiro ; Yamashiro, Takashi. / Runx/Cbfb signaling regulates postnatal development of granular convoluted tubule in the mouse submandibular gland. In: Developmental Dynamics. 2015 ; Vol. 244, No. 3. pp. 488-496.
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