TY - JOUR
T1 - RT-PCR analysis of Tecta, Coch, Eya4 and Strc in mouse cochlear explants
AU - Maeda, Yukihide
AU - Fukushima, Kunihiro
AU - Kakiuchi, Masashi
AU - Orita, Yorihisa
AU - Nishizaki, Kazunori
AU - Smith, Richard J.H.
PY - 2005/3/15
Y1 - 2005/3/15
N2 - Tecta, Coch, Eya4 and Strc are mouse orthologs of four human deafness-associated genes. Their expression is markedly restricted to specific cell types in cochleae. Cochleae were dissected on embryonic day 15 and cultured in vitro. Relative messenger RNA abundance of each gene was quantified by RT-PCR and compared in-vivo cochleae of equivalent embryonic age. After 48 h in culture, in-vivo and explant Strc expression levels were equivalent, Eya4 level reduced in explanted tissues, and expression of Tecta and Coch did not show the expected temporal rise. Expression of these genes was detectable even after 96 h. These results suggest that it is feasible to test the expression of inner ear specific genes in explanted cochleae.
AB - Tecta, Coch, Eya4 and Strc are mouse orthologs of four human deafness-associated genes. Their expression is markedly restricted to specific cell types in cochleae. Cochleae were dissected on embryonic day 15 and cultured in vitro. Relative messenger RNA abundance of each gene was quantified by RT-PCR and compared in-vivo cochleae of equivalent embryonic age. After 48 h in culture, in-vivo and explant Strc expression levels were equivalent, Eya4 level reduced in explanted tissues, and expression of Tecta and Coch did not show the expected temporal rise. Expression of these genes was detectable even after 96 h. These results suggest that it is feasible to test the expression of inner ear specific genes in explanted cochleae.
KW - Deafness-associated genes
KW - Embryonic cochlea
KW - Organ culture of cochlea
KW - Quantitative RT-PCR
UR - http://www.scopus.com/inward/record.url?scp=15944415844&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=15944415844&partnerID=8YFLogxK
U2 - 10.1097/00001756-200503150-00011
DO - 10.1097/00001756-200503150-00011
M3 - Article
C2 - 15729138
AN - SCOPUS:15944415844
VL - 16
SP - 361
EP - 365
JO - NeuroReport
JF - NeuroReport
SN - 0959-4965
IS - 4
ER -