RT-PCR analysis of Tecta, Coch, Eya4 and Strc in mouse cochlear explants

Yukihide Maeda; Kunihiro Fukushima; Masashi Kakiuchi; Yorihisa Orita; Kazunori Nishizaki; Richard J.H. Smith

doi:10.1097/00001756-200503150-00011

RT-PCR analysis of Tecta, Coch, Eya4 and Strc in mouse cochlear explants

Yukihide Maeda, Kunihiro Fukushima, Masashi Kakiuchi, Yorihisa Orita, Kazunori Nishizaki, Richard J.H. Smith

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

Abstract

Tecta, Coch, Eya4 and Strc are mouse orthologs of four human deafness-associated genes. Their expression is markedly restricted to specific cell types in cochleae. Cochleae were dissected on embryonic day 15 and cultured in vitro. Relative messenger RNA abundance of each gene was quantified by RT-PCR and compared in-vivo cochleae of equivalent embryonic age. After 48 h in culture, in-vivo and explant Strc expression levels were equivalent, Eya4 level reduced in explanted tissues, and expression of Tecta and Coch did not show the expected temporal rise. Expression of these genes was detectable even after 96 h. These results suggest that it is feasible to test the expression of inner ear specific genes in explanted cochleae.

Original language	English
Pages (from-to)	361-365
Number of pages	5
Journal	NeuroReport
Volume	16
Issue number	4
DOIs	https://doi.org/10.1097/00001756-200503150-00011
Publication status	Published - Mar 15 2005

Keywords

Deafness-associated genes
Embryonic cochlea
Organ culture of cochlea
Quantitative RT-PCR

ASJC Scopus subject areas

Neuroscience(all)

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title = "RT-PCR analysis of Tecta, Coch, Eya4 and Strc in mouse cochlear explants",

abstract = "Tecta, Coch, Eya4 and Strc are mouse orthologs of four human deafness-associated genes. Their expression is markedly restricted to specific cell types in cochleae. Cochleae were dissected on embryonic day 15 and cultured in vitro. Relative messenger RNA abundance of each gene was quantified by RT-PCR and compared in-vivo cochleae of equivalent embryonic age. After 48 h in culture, in-vivo and explant Strc expression levels were equivalent, Eya4 level reduced in explanted tissues, and expression of Tecta and Coch did not show the expected temporal rise. Expression of these genes was detectable even after 96 h. These results suggest that it is feasible to test the expression of inner ear specific genes in explanted cochleae.",

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T1 - RT-PCR analysis of Tecta, Coch, Eya4 and Strc in mouse cochlear explants

AU - Maeda, Yukihide

AU - Fukushima, Kunihiro

AU - Kakiuchi, Masashi

AU - Orita, Yorihisa

AU - Nishizaki, Kazunori

AU - Smith, Richard J.H.

PY - 2005/3/15

Y1 - 2005/3/15

N2 - Tecta, Coch, Eya4 and Strc are mouse orthologs of four human deafness-associated genes. Their expression is markedly restricted to specific cell types in cochleae. Cochleae were dissected on embryonic day 15 and cultured in vitro. Relative messenger RNA abundance of each gene was quantified by RT-PCR and compared in-vivo cochleae of equivalent embryonic age. After 48 h in culture, in-vivo and explant Strc expression levels were equivalent, Eya4 level reduced in explanted tissues, and expression of Tecta and Coch did not show the expected temporal rise. Expression of these genes was detectable even after 96 h. These results suggest that it is feasible to test the expression of inner ear specific genes in explanted cochleae.

AB - Tecta, Coch, Eya4 and Strc are mouse orthologs of four human deafness-associated genes. Their expression is markedly restricted to specific cell types in cochleae. Cochleae were dissected on embryonic day 15 and cultured in vitro. Relative messenger RNA abundance of each gene was quantified by RT-PCR and compared in-vivo cochleae of equivalent embryonic age. After 48 h in culture, in-vivo and explant Strc expression levels were equivalent, Eya4 level reduced in explanted tissues, and expression of Tecta and Coch did not show the expected temporal rise. Expression of these genes was detectable even after 96 h. These results suggest that it is feasible to test the expression of inner ear specific genes in explanted cochleae.

KW - Deafness-associated genes

KW - Embryonic cochlea

KW - Organ culture of cochlea

KW - Quantitative RT-PCR

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RT-PCR analysis of Tecta, Coch, Eya4 and Strc in mouse cochlear explants

Abstract

Keywords

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