Abstract
It has been reported that the Golgi apparatus (GA) is fragmented in some neurodegenerative diseases. However, the significance of the GA fragmentation or disassembly in neurodegeneration is still obscure. To clarify the involvement of this organelle in apoptosis of neuronal cells, we examined the morphological changes in the GA induced by rotenone, a pesticide that produces selective dopaminergic neurodegeneration. In dopaminergic neuroblastoma B65 cells, a 5-day rotenone treatment (50 nM) promoted cell damage. Rotenone-treated cells showed round nuclei, diffuse signals of the GA and cytosolic redistribution of cytochrome c. Nevertheless, these type of cells without nuclear fragmentation did not show any caspase-3 expression. These results indicate that rotenone induces disassembly of the GA in the early stages of the apoptotic process.
| Original language | English |
|---|---|
| Pages (from-to) | 59-63 |
| Number of pages | 5 |
| Journal | Neuroscience Letters |
| Volume | 354 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - Jan 2 2004 |
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Keywords
- Apoptosis
- Disassembly of the Golgi apparatus
- Endoplasmic reticulum stress
- Neurodegeneration
- Parkinsonism
- Rotenone
ASJC Scopus subject areas
- Neuroscience(all)
Cite this
Rotenone induces disassembly of the Golgi apparatus in the rat dopaminergic neuroblastoma B65 cell line. / Diaz-Corrales, Francisco J.; Asanuma, Masato; Miyazaki, Ikuko; Ogawa, Norio.
In: Neuroscience Letters, Vol. 354, No. 1, 02.01.2004, p. 59-63.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Rotenone induces disassembly of the Golgi apparatus in the rat dopaminergic neuroblastoma B65 cell line
AU - Diaz-Corrales, Francisco J.
AU - Asanuma, Masato
AU - Miyazaki, Ikuko
AU - Ogawa, Norio
PY - 2004/1/2
Y1 - 2004/1/2
N2 - It has been reported that the Golgi apparatus (GA) is fragmented in some neurodegenerative diseases. However, the significance of the GA fragmentation or disassembly in neurodegeneration is still obscure. To clarify the involvement of this organelle in apoptosis of neuronal cells, we examined the morphological changes in the GA induced by rotenone, a pesticide that produces selective dopaminergic neurodegeneration. In dopaminergic neuroblastoma B65 cells, a 5-day rotenone treatment (50 nM) promoted cell damage. Rotenone-treated cells showed round nuclei, diffuse signals of the GA and cytosolic redistribution of cytochrome c. Nevertheless, these type of cells without nuclear fragmentation did not show any caspase-3 expression. These results indicate that rotenone induces disassembly of the GA in the early stages of the apoptotic process.
AB - It has been reported that the Golgi apparatus (GA) is fragmented in some neurodegenerative diseases. However, the significance of the GA fragmentation or disassembly in neurodegeneration is still obscure. To clarify the involvement of this organelle in apoptosis of neuronal cells, we examined the morphological changes in the GA induced by rotenone, a pesticide that produces selective dopaminergic neurodegeneration. In dopaminergic neuroblastoma B65 cells, a 5-day rotenone treatment (50 nM) promoted cell damage. Rotenone-treated cells showed round nuclei, diffuse signals of the GA and cytosolic redistribution of cytochrome c. Nevertheless, these type of cells without nuclear fragmentation did not show any caspase-3 expression. These results indicate that rotenone induces disassembly of the GA in the early stages of the apoptotic process.
KW - Apoptosis
KW - Disassembly of the Golgi apparatus
KW - Endoplasmic reticulum stress
KW - Neurodegeneration
KW - Parkinsonism
KW - Rotenone
UR - http://www.scopus.com/inward/record.url?scp=0347416824&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0347416824&partnerID=8YFLogxK
U2 - 10.1016/j.neulet.2003.09.059
DO - 10.1016/j.neulet.2003.09.059
M3 - Article
C2 - 14698482
AN - SCOPUS:0347416824
VL - 354
SP - 59
EP - 63
JO - Neuroscience Letters
JF - Neuroscience Letters
SN - 0304-3940
IS - 1
ER -