Root-specific role for nicotiana benthamiana RDR6 in the inhibition of chinese wheat mosaic virus accumulation at higher temperatures

Andika Ida Bagus, Liying Sun, Rong Xiang, Junmin Li, Jianping Chen

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Some viruses only infect plants at cool temperatures but the molecular mechanism underlying this low-temperature dependence remains unclear. Chinese wheat mosaic virus (CWMV, genus Furovirus) was able to infect wheat and Nicotiana benthamiana plants at 16 but not at 24°C. When CWMV-infected plants were transferred to 24°C for 2 weeks, the newly emerged leaves and roots became virus free. Co-infection with Potato virus Y rescued CWMV accumulation in N. benthamiana plants after a temperature shift to 24°C. In transgenic N. benthamiana plants silenced for the N. benthamiana RNA-dependent RNA polymerase 6 (NbRDR6), CWMV was able to accumulate in roots but not in leaves after a temperature shift to 24°C. Deep sequencing of small RNAs showed that, at 16°C, abundant CWMV small interfering (si)RNAs accumulated in infected N. benthamiana plants. Silencing of NbRDR6 increased the abundance of CWMV siRNAs and the generation of siRNAs from hotspots in the CWMV genome. In contrast, when shifted to 24°C for 1 week, CWMV siRNAs were markedly fewer in roots of NbRDR6-silenced than in roots of wildtype plants but were similar in the leaves of those plants. Our results demonstrate the root-specific role of NbRDR6 in the inhibition of CWMV accumulation and biogenesis of CWMV siRNAs at higher temperatures.

Original languageEnglish
Pages (from-to)1165-1175
Number of pages11
JournalMolecular Plant-Microbe Interactions
Volume26
Issue number10
DOIs
Publication statusPublished - Oct 2013
Externally publishedYes

Fingerprint

Chinese wheat mosaic virus
Mosaic Viruses
Nicotiana benthamiana
Triticum
Tobacco
RNA Replicase
RNA-directed RNA polymerase
Temperature
temperature
Potyvirus
Viruses
High-Throughput Nucleotide Sequencing
Furovirus
Plant Roots
Plant Leaves
Coinfection
leaves
viruses
Small Interfering RNA
Potato virus Y

ASJC Scopus subject areas

  • Physiology
  • Agronomy and Crop Science

Cite this

Root-specific role for nicotiana benthamiana RDR6 in the inhibition of chinese wheat mosaic virus accumulation at higher temperatures. / Ida Bagus, Andika; Sun, Liying; Xiang, Rong; Li, Junmin; Chen, Jianping.

In: Molecular Plant-Microbe Interactions, Vol. 26, No. 10, 10.2013, p. 1165-1175.

Research output: Contribution to journalArticle

Ida Bagus, Andika ; Sun, Liying ; Xiang, Rong ; Li, Junmin ; Chen, Jianping. / Root-specific role for nicotiana benthamiana RDR6 in the inhibition of chinese wheat mosaic virus accumulation at higher temperatures. In: Molecular Plant-Microbe Interactions. 2013 ; Vol. 26, No. 10. pp. 1165-1175.
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abstract = "Some viruses only infect plants at cool temperatures but the molecular mechanism underlying this low-temperature dependence remains unclear. Chinese wheat mosaic virus (CWMV, genus Furovirus) was able to infect wheat and Nicotiana benthamiana plants at 16 but not at 24°C. When CWMV-infected plants were transferred to 24°C for 2 weeks, the newly emerged leaves and roots became virus free. Co-infection with Potato virus Y rescued CWMV accumulation in N. benthamiana plants after a temperature shift to 24°C. In transgenic N. benthamiana plants silenced for the N. benthamiana RNA-dependent RNA polymerase 6 (NbRDR6), CWMV was able to accumulate in roots but not in leaves after a temperature shift to 24°C. Deep sequencing of small RNAs showed that, at 16°C, abundant CWMV small interfering (si)RNAs accumulated in infected N. benthamiana plants. Silencing of NbRDR6 increased the abundance of CWMV siRNAs and the generation of siRNAs from hotspots in the CWMV genome. In contrast, when shifted to 24°C for 1 week, CWMV siRNAs were markedly fewer in roots of NbRDR6-silenced than in roots of wildtype plants but were similar in the leaves of those plants. Our results demonstrate the root-specific role of NbRDR6 in the inhibition of CWMV accumulation and biogenesis of CWMV siRNAs at higher temperatures.",
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