Root plasma membrane H+-ATPase is involved in the adaptation of soybean to phosphorus starvation

Hong Shen, Jianhong Chen, Zhanyi Wang, Cunyi Yang, Takara Sasaki, Yoko Yamamoto, Hideaki Matsumoto, Xiaolong Yan

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88 Citations (Scopus)

Abstract

The plasma membrane H+-ATPase plays an important role in the plant response to nutrient and environmental stresses. However, the involvement of plant root plasma membrane H+-ATPase in adaptation to phosphate (P) starvation is not yet fully elucidated. In this study, experiments were performed with soybean roots in low-P nutrient solution (10 μM). Treatment with fusicoccin, an activator of the plasma membrane H+-ATPase, increased P uptake by 35%, while vanadate, an inhibitor of plasma membrane H+-ATPase, severely suppressed it. These results suggested that P uptake might be regulated via the modulation of the activity of plasma membrane H+-ATPase under P starvation. The relationship between P uptake and the activity of plasma membrane H+-ATPase was examined further by using plasma membrane H+-ATPase transgenic Arabidopsis thaliana under low-P conditions. Transgenic plants absorbed more P compared with wild-type Arabidopsis. Results from real-time RT-PCR, western-blotting and immunolocalization analysis indicated that the increase in activity of the plasma membrane H+-ATPase by P starvation was caused by its transcriptional and translational regulation. A higher expression was observed at the translational level than at the transcriptional level. P starvation could induce a transient increase of endogenous indole-3-acetic acid (IAA) in soybean roots. The exogenous application of IAA stimulated the activity of plasma membrane H+-ATPase and P uptake, while naphthylphthalamic acid (NPA), an IAA transport inhibitor, blocked IAA effects. Taken together, these results suggested an involvement of root plasma membrane H+-ATPase in the adaptation of soybean to P starvation. IAA might be involved in signal transduction of P starvation by activating the plasma membrane H +-ATPase in soybean roots.

Original languageEnglish
Pages (from-to)1353-1362
Number of pages10
JournalJournal of experimental botany
Volume57
Issue number6
DOIs
Publication statusPublished - Apr 19 2006

Keywords

  • IAA
  • P starvation
  • P uptake
  • Plasma membrane H-ATPase
  • Soybean roots

ASJC Scopus subject areas

  • Physiology
  • Plant Science

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    Shen, H., Chen, J., Wang, Z., Yang, C., Sasaki, T., Yamamoto, Y., Matsumoto, H., & Yan, X. (2006). Root plasma membrane H+-ATPase is involved in the adaptation of soybean to phosphorus starvation. Journal of experimental botany, 57(6), 1353-1362. https://doi.org/10.1093/jxb/erj111