Root plasma membrane H+-ATPase is involved in the adaptation of soybean to phosphorus starvation

Hong Shen, Jianhong Chen, Zhanyi Wang, Cunyi Yang, Takara Sasaki, Yoko Yamamoto, Hideaki Matsumoto, Xiaolong Yan

    Research output: Contribution to journalArticlepeer-review

    95 Citations (Scopus)

    Abstract

    The plasma membrane H+-ATPase plays an important role in the plant response to nutrient and environmental stresses. However, the involvement of plant root plasma membrane H+-ATPase in adaptation to phosphate (P) starvation is not yet fully elucidated. In this study, experiments were performed with soybean roots in low-P nutrient solution (10 μM). Treatment with fusicoccin, an activator of the plasma membrane H+-ATPase, increased P uptake by 35%, while vanadate, an inhibitor of plasma membrane H+-ATPase, severely suppressed it. These results suggested that P uptake might be regulated via the modulation of the activity of plasma membrane H+-ATPase under P starvation. The relationship between P uptake and the activity of plasma membrane H+-ATPase was examined further by using plasma membrane H+-ATPase transgenic Arabidopsis thaliana under low-P conditions. Transgenic plants absorbed more P compared with wild-type Arabidopsis. Results from real-time RT-PCR, western-blotting and immunolocalization analysis indicated that the increase in activity of the plasma membrane H+-ATPase by P starvation was caused by its transcriptional and translational regulation. A higher expression was observed at the translational level than at the transcriptional level. P starvation could induce a transient increase of endogenous indole-3-acetic acid (IAA) in soybean roots. The exogenous application of IAA stimulated the activity of plasma membrane H+-ATPase and P uptake, while naphthylphthalamic acid (NPA), an IAA transport inhibitor, blocked IAA effects. Taken together, these results suggested an involvement of root plasma membrane H+-ATPase in the adaptation of soybean to P starvation. IAA might be involved in signal transduction of P starvation by activating the plasma membrane H +-ATPase in soybean roots.

    Original languageEnglish
    Pages (from-to)1353-1362
    Number of pages10
    JournalJournal of experimental botany
    Volume57
    Issue number6
    DOIs
    Publication statusPublished - 2006

    Keywords

    • IAA
    • P starvation
    • P uptake
    • Plasma membrane H-ATPase
    • Soybean roots

    ASJC Scopus subject areas

    • Physiology
    • Plant Science

    Fingerprint

    Dive into the research topics of 'Root plasma membrane H<sup>+</sup>-ATPase is involved in the adaptation of soybean to phosphorus starvation'. Together they form a unique fingerprint.

    Cite this