Root plasma membrane H+-ATPase is involved in the adaptation of soybean to phosphorus starvation

Hong Shen, Jianhong Chen, Zhanyi Wang, Cunyi Yang, Takara Sasaki, Yoko Yamamoto, Hideaki Matsumoto, Xiaolong Yan

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Abstract

The plasma membrane H+-ATPase plays an important role in the plant response to nutrient and environmental stresses. However, the involvement of plant root plasma membrane H+-ATPase in adaptation to phosphate (P) starvation is not yet fully elucidated. In this study, experiments were performed with soybean roots in low-P nutrient solution (10 μM). Treatment with fusicoccin, an activator of the plasma membrane H+-ATPase, increased P uptake by 35%, while vanadate, an inhibitor of plasma membrane H+-ATPase, severely suppressed it. These results suggested that P uptake might be regulated via the modulation of the activity of plasma membrane H+-ATPase under P starvation. The relationship between P uptake and the activity of plasma membrane H+-ATPase was examined further by using plasma membrane H+-ATPase transgenic Arabidopsis thaliana under low-P conditions. Transgenic plants absorbed more P compared with wild-type Arabidopsis. Results from real-time RT-PCR, western-blotting and immunolocalization analysis indicated that the increase in activity of the plasma membrane H+-ATPase by P starvation was caused by its transcriptional and translational regulation. A higher expression was observed at the translational level than at the transcriptional level. P starvation could induce a transient increase of endogenous indole-3-acetic acid (IAA) in soybean roots. The exogenous application of IAA stimulated the activity of plasma membrane H+-ATPase and P uptake, while naphthylphthalamic acid (NPA), an IAA transport inhibitor, blocked IAA effects. Taken together, these results suggested an involvement of root plasma membrane H+-ATPase in the adaptation of soybean to P starvation. IAA might be involved in signal transduction of P starvation by activating the plasma membrane H +-ATPase in soybean roots.

Original languageEnglish
Pages (from-to)1353-1362
Number of pages10
JournalJournal of Experimental Botany
Volume57
Issue number6
DOIs
Publication statusPublished - 2006

Fingerprint

H-transporting ATP synthase
Proton-Translocating ATPases
Starvation
Soybeans
Phosphorus
starvation
plasma membrane
Cell Membrane
soybeans
phosphorus
indole acetic acid
uptake mechanisms
Arabidopsis
naphthylphthalamic acid
fusicoccin
Plant Roots
Vanadates
Genetically Modified Plants
nutrient solutions
plant response

Keywords

  • IAA
  • P starvation
  • P uptake
  • Plasma membrane H-ATPase
  • Soybean roots

ASJC Scopus subject areas

  • Plant Science

Cite this

Shen, H., Chen, J., Wang, Z., Yang, C., Sasaki, T., Yamamoto, Y., ... Yan, X. (2006). Root plasma membrane H+-ATPase is involved in the adaptation of soybean to phosphorus starvation. Journal of Experimental Botany, 57(6), 1353-1362. https://doi.org/10.1093/jxb/erj111

Root plasma membrane H+-ATPase is involved in the adaptation of soybean to phosphorus starvation. / Shen, Hong; Chen, Jianhong; Wang, Zhanyi; Yang, Cunyi; Sasaki, Takara; Yamamoto, Yoko; Matsumoto, Hideaki; Yan, Xiaolong.

In: Journal of Experimental Botany, Vol. 57, No. 6, 2006, p. 1353-1362.

Research output: Contribution to journalArticle

Shen, H, Chen, J, Wang, Z, Yang, C, Sasaki, T, Yamamoto, Y, Matsumoto, H & Yan, X 2006, 'Root plasma membrane H+-ATPase is involved in the adaptation of soybean to phosphorus starvation', Journal of Experimental Botany, vol. 57, no. 6, pp. 1353-1362. https://doi.org/10.1093/jxb/erj111
Shen, Hong ; Chen, Jianhong ; Wang, Zhanyi ; Yang, Cunyi ; Sasaki, Takara ; Yamamoto, Yoko ; Matsumoto, Hideaki ; Yan, Xiaolong. / Root plasma membrane H+-ATPase is involved in the adaptation of soybean to phosphorus starvation. In: Journal of Experimental Botany. 2006 ; Vol. 57, No. 6. pp. 1353-1362.
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AU - Yamamoto, Yoko

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AU - Yan, Xiaolong

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AB - The plasma membrane H+-ATPase plays an important role in the plant response to nutrient and environmental stresses. However, the involvement of plant root plasma membrane H+-ATPase in adaptation to phosphate (P) starvation is not yet fully elucidated. In this study, experiments were performed with soybean roots in low-P nutrient solution (10 μM). Treatment with fusicoccin, an activator of the plasma membrane H+-ATPase, increased P uptake by 35%, while vanadate, an inhibitor of plasma membrane H+-ATPase, severely suppressed it. These results suggested that P uptake might be regulated via the modulation of the activity of plasma membrane H+-ATPase under P starvation. The relationship between P uptake and the activity of plasma membrane H+-ATPase was examined further by using plasma membrane H+-ATPase transgenic Arabidopsis thaliana under low-P conditions. Transgenic plants absorbed more P compared with wild-type Arabidopsis. Results from real-time RT-PCR, western-blotting and immunolocalization analysis indicated that the increase in activity of the plasma membrane H+-ATPase by P starvation was caused by its transcriptional and translational regulation. A higher expression was observed at the translational level than at the transcriptional level. P starvation could induce a transient increase of endogenous indole-3-acetic acid (IAA) in soybean roots. The exogenous application of IAA stimulated the activity of plasma membrane H+-ATPase and P uptake, while naphthylphthalamic acid (NPA), an IAA transport inhibitor, blocked IAA effects. Taken together, these results suggested an involvement of root plasma membrane H+-ATPase in the adaptation of soybean to P starvation. IAA might be involved in signal transduction of P starvation by activating the plasma membrane H +-ATPase in soybean roots.

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