Root hairless 2 (RTH2) mutant represents a loss-of-function allele of the cellulose synthase-like gene OSCSLD1 in rice (Oryza sativa L.)

Takahisa Yuo, Kenji Shiotani, Naoki Shitsukawa, Akio Miyao, Hirohiko Hirochika, Masahiko Ichii, Shin Taketa

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Root hair is considered to play important roles in water and nutrient uptake and anchoring the plant to the soil. To gain further knowledge of root hair morphogenesis in rice, we isolated root hairless 2 (rth2) mutant from the mutant panel of Nipponbare. Positional cloning and complementation test revealed that the causal gene of rth2 was Cellulose Synthase-Like D1 (OsCSLD1). rth2 has a premature stop codon in exon 1 as a result of two consecutive nucleotide substitutions and is predicted to produce truncated proteins lacking the D, D, D, QxxRW motif and 8 transmembrane domains. In rth2, bulges were normally initiated from asymmetric divisions of root epidermal cells, but bulges did not elongate. Therefore, rth2 shows completely roothairless phenotype. qRT-PCR analysis revealed that OsCSLD1 was expressed not only in root but also in shoot. In situ hybridization showed that OsCSLD1 was expressed not only in root hairs but also in epidermal and cortex cell walls except for stele. Agronomic character evaluation in pot experiments showed that rth2 did not differ significantly from Nipponbare in all characters examined except for root dry weight, which showed a significant increase in rth2. In paddy field experiment, rth2 was significantly inferior compared with Nipponbare in agronomic performance.

Original languageEnglish
Pages (from-to)225-233
Number of pages9
JournalBreeding Science
Volume61
Issue number3
DOIs
Publication statusPublished - 2011

Fingerprint

cellulose synthase
Oryza sativa
Alleles
alleles
rice
mutants
Genetic Complementation Test
Genes
genes
Nonsense Codon
Morphogenesis
Cell Wall
In Situ Hybridization
root hairs
Organism Cloning
Exons
Soil
Nucleotides
Phenotype
Weights and Measures

Keywords

  • Bulge
  • Gene
  • Positional cloning
  • Root hair
  • Tos17

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Genetics
  • Plant Science

Cite this

Root hairless 2 (RTH2) mutant represents a loss-of-function allele of the cellulose synthase-like gene OSCSLD1 in rice (Oryza sativa L.). / Yuo, Takahisa; Shiotani, Kenji; Shitsukawa, Naoki; Miyao, Akio; Hirochika, Hirohiko; Ichii, Masahiko; Taketa, Shin.

In: Breeding Science, Vol. 61, No. 3, 2011, p. 225-233.

Research output: Contribution to journalArticle

Yuo, Takahisa ; Shiotani, Kenji ; Shitsukawa, Naoki ; Miyao, Akio ; Hirochika, Hirohiko ; Ichii, Masahiko ; Taketa, Shin. / Root hairless 2 (RTH2) mutant represents a loss-of-function allele of the cellulose synthase-like gene OSCSLD1 in rice (Oryza sativa L.). In: Breeding Science. 2011 ; Vol. 61, No. 3. pp. 225-233.
@article{5ef235fa199d407fac681d71bcd86c87,
title = "Root hairless 2 (RTH2) mutant represents a loss-of-function allele of the cellulose synthase-like gene OSCSLD1 in rice (Oryza sativa L.)",
abstract = "Root hair is considered to play important roles in water and nutrient uptake and anchoring the plant to the soil. To gain further knowledge of root hair morphogenesis in rice, we isolated root hairless 2 (rth2) mutant from the mutant panel of Nipponbare. Positional cloning and complementation test revealed that the causal gene of rth2 was Cellulose Synthase-Like D1 (OsCSLD1). rth2 has a premature stop codon in exon 1 as a result of two consecutive nucleotide substitutions and is predicted to produce truncated proteins lacking the D, D, D, QxxRW motif and 8 transmembrane domains. In rth2, bulges were normally initiated from asymmetric divisions of root epidermal cells, but bulges did not elongate. Therefore, rth2 shows completely roothairless phenotype. qRT-PCR analysis revealed that OsCSLD1 was expressed not only in root but also in shoot. In situ hybridization showed that OsCSLD1 was expressed not only in root hairs but also in epidermal and cortex cell walls except for stele. Agronomic character evaluation in pot experiments showed that rth2 did not differ significantly from Nipponbare in all characters examined except for root dry weight, which showed a significant increase in rth2. In paddy field experiment, rth2 was significantly inferior compared with Nipponbare in agronomic performance.",
keywords = "Bulge, Gene, Positional cloning, Root hair, Tos17",
author = "Takahisa Yuo and Kenji Shiotani and Naoki Shitsukawa and Akio Miyao and Hirohiko Hirochika and Masahiko Ichii and Shin Taketa",
year = "2011",
doi = "10.1270/jsbbs.61.225",
language = "English",
volume = "61",
pages = "225--233",
journal = "Breeding Science",
issn = "1344-7610",
publisher = "Japanese Society of Breeding",
number = "3",

}

TY - JOUR

T1 - Root hairless 2 (RTH2) mutant represents a loss-of-function allele of the cellulose synthase-like gene OSCSLD1 in rice (Oryza sativa L.)

AU - Yuo, Takahisa

AU - Shiotani, Kenji

AU - Shitsukawa, Naoki

AU - Miyao, Akio

AU - Hirochika, Hirohiko

AU - Ichii, Masahiko

AU - Taketa, Shin

PY - 2011

Y1 - 2011

N2 - Root hair is considered to play important roles in water and nutrient uptake and anchoring the plant to the soil. To gain further knowledge of root hair morphogenesis in rice, we isolated root hairless 2 (rth2) mutant from the mutant panel of Nipponbare. Positional cloning and complementation test revealed that the causal gene of rth2 was Cellulose Synthase-Like D1 (OsCSLD1). rth2 has a premature stop codon in exon 1 as a result of two consecutive nucleotide substitutions and is predicted to produce truncated proteins lacking the D, D, D, QxxRW motif and 8 transmembrane domains. In rth2, bulges were normally initiated from asymmetric divisions of root epidermal cells, but bulges did not elongate. Therefore, rth2 shows completely roothairless phenotype. qRT-PCR analysis revealed that OsCSLD1 was expressed not only in root but also in shoot. In situ hybridization showed that OsCSLD1 was expressed not only in root hairs but also in epidermal and cortex cell walls except for stele. Agronomic character evaluation in pot experiments showed that rth2 did not differ significantly from Nipponbare in all characters examined except for root dry weight, which showed a significant increase in rth2. In paddy field experiment, rth2 was significantly inferior compared with Nipponbare in agronomic performance.

AB - Root hair is considered to play important roles in water and nutrient uptake and anchoring the plant to the soil. To gain further knowledge of root hair morphogenesis in rice, we isolated root hairless 2 (rth2) mutant from the mutant panel of Nipponbare. Positional cloning and complementation test revealed that the causal gene of rth2 was Cellulose Synthase-Like D1 (OsCSLD1). rth2 has a premature stop codon in exon 1 as a result of two consecutive nucleotide substitutions and is predicted to produce truncated proteins lacking the D, D, D, QxxRW motif and 8 transmembrane domains. In rth2, bulges were normally initiated from asymmetric divisions of root epidermal cells, but bulges did not elongate. Therefore, rth2 shows completely roothairless phenotype. qRT-PCR analysis revealed that OsCSLD1 was expressed not only in root but also in shoot. In situ hybridization showed that OsCSLD1 was expressed not only in root hairs but also in epidermal and cortex cell walls except for stele. Agronomic character evaluation in pot experiments showed that rth2 did not differ significantly from Nipponbare in all characters examined except for root dry weight, which showed a significant increase in rth2. In paddy field experiment, rth2 was significantly inferior compared with Nipponbare in agronomic performance.

KW - Bulge

KW - Gene

KW - Positional cloning

KW - Root hair

KW - Tos17

UR - http://www.scopus.com/inward/record.url?scp=80052758069&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=80052758069&partnerID=8YFLogxK

U2 - 10.1270/jsbbs.61.225

DO - 10.1270/jsbbs.61.225

M3 - Article

AN - SCOPUS:80052758069

VL - 61

SP - 225

EP - 233

JO - Breeding Science

JF - Breeding Science

SN - 1344-7610

IS - 3

ER -