Root cone angle is enlarged in docs1 LRR-RLK mutants in rice

M. Bettembourg, M. Dal-Soglio, C. Bureau, A. Vernet, A. Dardoux, M. Portefaix, M. Bes, D. Meynard, D. Mieulet, B. Cayrol, C. Perin, B. Courtois, Jian Feng Ma, A. Dievart

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Background: The DEFECTIVE IN OUTER CELL LAYER SPECIFICATION 1 (DOCS1) gene belongs to the Leucine-Rich Repeat Receptor-Like Kinase (LRR-RLK) subfamily. It has been discovered few years ago in Oryza sativa (rice) in a screen to isolate mutants with defects in sensitivity to aluminum. The c68 (docs1–1) mutant possessed a nonsense mutation in the C-terminal part of the DOCS1 kinase domain. Findings: We have generated a new loss-of-function mutation in the DOCS1 gene (docs1–2) using the CRISPR-Cas9 technology. This new loss-of-function mutant and docs1–1 present similar phenotypes suggesting the original docs1–1 was a null allele. Besides the aluminum sensitivity phenotype, both docs1 mutants shared also several root phenotypes described previously: less root hairs and mixed identities of the outer cell layers. Moreover, our new results suggest that DOCS1 could also play a role in root cap development. We hypothesized these docs1 root phenotypes may affect gravity responses. As expected, in seedlings, the early gravitropic response was delayed. Furthermore, at adult stage, the root gravitropic set angle of docs1 mutants was also affected since docs1 mutant plants displayed larger root cone angles. Conclusions: All these observations add new insights into the DOCS1 gene function in gravitropic responses at several stages of plant development.

Original languageEnglish
Article number50
JournalRice
Volume10
Issue number1
DOIs
Publication statusPublished - Dec 1 2017

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seed cones
leucine
phosphotransferases (kinases)
rice
mutants
receptors
phenotype
cells
aluminum
gene
mutation
nonsense mutation
root cap
genes
null alleles
root hairs
hair
gravity
defect
plant development

Keywords

  • Angle
  • docs1
  • Gravitropism
  • GSA
  • Lrr
  • RCA
  • RGA
  • Rice
  • Rlk
  • Root cap

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Soil Science
  • Plant Science

Cite this

Bettembourg, M., Dal-Soglio, M., Bureau, C., Vernet, A., Dardoux, A., Portefaix, M., ... Dievart, A. (2017). Root cone angle is enlarged in docs1 LRR-RLK mutants in rice. Rice, 10(1), [50]. https://doi.org/10.1186/s12284-017-0190-1

Root cone angle is enlarged in docs1 LRR-RLK mutants in rice. / Bettembourg, M.; Dal-Soglio, M.; Bureau, C.; Vernet, A.; Dardoux, A.; Portefaix, M.; Bes, M.; Meynard, D.; Mieulet, D.; Cayrol, B.; Perin, C.; Courtois, B.; Ma, Jian Feng; Dievart, A.

In: Rice, Vol. 10, No. 1, 50, 01.12.2017.

Research output: Contribution to journalArticle

Bettembourg, M, Dal-Soglio, M, Bureau, C, Vernet, A, Dardoux, A, Portefaix, M, Bes, M, Meynard, D, Mieulet, D, Cayrol, B, Perin, C, Courtois, B, Ma, JF & Dievart, A 2017, 'Root cone angle is enlarged in docs1 LRR-RLK mutants in rice', Rice, vol. 10, no. 1, 50. https://doi.org/10.1186/s12284-017-0190-1
Bettembourg M, Dal-Soglio M, Bureau C, Vernet A, Dardoux A, Portefaix M et al. Root cone angle is enlarged in docs1 LRR-RLK mutants in rice. Rice. 2017 Dec 1;10(1). 50. https://doi.org/10.1186/s12284-017-0190-1
Bettembourg, M. ; Dal-Soglio, M. ; Bureau, C. ; Vernet, A. ; Dardoux, A. ; Portefaix, M. ; Bes, M. ; Meynard, D. ; Mieulet, D. ; Cayrol, B. ; Perin, C. ; Courtois, B. ; Ma, Jian Feng ; Dievart, A. / Root cone angle is enlarged in docs1 LRR-RLK mutants in rice. In: Rice. 2017 ; Vol. 10, No. 1.
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abstract = "Background: The DEFECTIVE IN OUTER CELL LAYER SPECIFICATION 1 (DOCS1) gene belongs to the Leucine-Rich Repeat Receptor-Like Kinase (LRR-RLK) subfamily. It has been discovered few years ago in Oryza sativa (rice) in a screen to isolate mutants with defects in sensitivity to aluminum. The c68 (docs1–1) mutant possessed a nonsense mutation in the C-terminal part of the DOCS1 kinase domain. Findings: We have generated a new loss-of-function mutation in the DOCS1 gene (docs1–2) using the CRISPR-Cas9 technology. This new loss-of-function mutant and docs1–1 present similar phenotypes suggesting the original docs1–1 was a null allele. Besides the aluminum sensitivity phenotype, both docs1 mutants shared also several root phenotypes described previously: less root hairs and mixed identities of the outer cell layers. Moreover, our new results suggest that DOCS1 could also play a role in root cap development. We hypothesized these docs1 root phenotypes may affect gravity responses. As expected, in seedlings, the early gravitropic response was delayed. Furthermore, at adult stage, the root gravitropic set angle of docs1 mutants was also affected since docs1 mutant plants displayed larger root cone angles. Conclusions: All these observations add new insights into the DOCS1 gene function in gravitropic responses at several stages of plant development.",
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AU - Bes, M.

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AB - Background: The DEFECTIVE IN OUTER CELL LAYER SPECIFICATION 1 (DOCS1) gene belongs to the Leucine-Rich Repeat Receptor-Like Kinase (LRR-RLK) subfamily. It has been discovered few years ago in Oryza sativa (rice) in a screen to isolate mutants with defects in sensitivity to aluminum. The c68 (docs1–1) mutant possessed a nonsense mutation in the C-terminal part of the DOCS1 kinase domain. Findings: We have generated a new loss-of-function mutation in the DOCS1 gene (docs1–2) using the CRISPR-Cas9 technology. This new loss-of-function mutant and docs1–1 present similar phenotypes suggesting the original docs1–1 was a null allele. Besides the aluminum sensitivity phenotype, both docs1 mutants shared also several root phenotypes described previously: less root hairs and mixed identities of the outer cell layers. Moreover, our new results suggest that DOCS1 could also play a role in root cap development. We hypothesized these docs1 root phenotypes may affect gravity responses. As expected, in seedlings, the early gravitropic response was delayed. Furthermore, at adult stage, the root gravitropic set angle of docs1 mutants was also affected since docs1 mutant plants displayed larger root cone angles. Conclusions: All these observations add new insights into the DOCS1 gene function in gravitropic responses at several stages of plant development.

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