Root and shoot transcriptome analysis of two ecotypes of Noccaea caerulescens uncovers the role of NcNramp1 in Cd hyperaccumulation

Matthew J. Milner, Namiki Mitani, Naoki Yamaji, Kengo Yokosho, Eric Craft, Zhangjun Fei, Stephen Ebbs, M. Clemencia Zambrano, Jian Feng Ma, Leon V. Kochian

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

The Zn/Cd hyperaccumulator, Noccaea caerulescens, has been studied extensively for its ability to accumulate high levels of Zn and Cd in its leaves. Previous studies have indicated that the Zn and Cd hyperaccumulation trait exhibited by this species involves different transport and tolerance mechanisms. It has also been well documented that certain ecotypes of N. caerulescens are much better Cd hyperaccumulators than others. However, there does not seem to be much ecotypic variation for Zn hyperaccumulation in N. caerulescens. In this study we employed a comparative transcriptomics approach to look at root and shoot gene expression in Ganges and Prayon plants in response to Cd stress to identify transporter genes that were more highly expressed in either the roots or shoots of the superior Cd accumulator, Ganges. Comparison of the transcriptomes from the two ecotypes of Noccaea caerulescens identified a number of genes that encoded metal transporters that were more highly expressed in the Ganges ecotype in response to Cd stress. Characterization of one of these transporters, NcNramp1, showed that it is involved in the influx of Cd across the endodermal plasma membrane and thus may play a key role in Cd flux into the stele and root-to-shoot Cd transport. NcNramp1 may be one of the main transporters involved in Cd hyperaccumulation in N. caerulescens and copy number variation appears to be the main reason for high NcNramp1 gene expression underlying the increased Cd accumulation in the Ganges ecotype.

Original languageEnglish
Pages (from-to)398-410
Number of pages13
JournalPlant Journal
Volume78
Issue number3
DOIs
Publication statusPublished - 2014

Fingerprint

Ecotype
Gene Expression Profiling
transcriptomics
ecotypes
transporters
shoots
hyperaccumulators
stress response
Gene Expression
gene expression
Transcriptome
stele
Genes
transcriptome
Metals
Cell Membrane
plant response
plasma membrane
genes
metals

Keywords

  • cadmium
  • Cd hyperaccumulation
  • Cd transport
  • hyperaccumulation
  • Noccaea caerulescens
  • Nramp

ASJC Scopus subject areas

  • Plant Science
  • Cell Biology
  • Genetics
  • Medicine(all)

Cite this

Root and shoot transcriptome analysis of two ecotypes of Noccaea caerulescens uncovers the role of NcNramp1 in Cd hyperaccumulation. / Milner, Matthew J.; Mitani, Namiki; Yamaji, Naoki; Yokosho, Kengo; Craft, Eric; Fei, Zhangjun; Ebbs, Stephen; Clemencia Zambrano, M.; Ma, Jian Feng; Kochian, Leon V.

In: Plant Journal, Vol. 78, No. 3, 2014, p. 398-410.

Research output: Contribution to journalArticle

Milner, Matthew J. ; Mitani, Namiki ; Yamaji, Naoki ; Yokosho, Kengo ; Craft, Eric ; Fei, Zhangjun ; Ebbs, Stephen ; Clemencia Zambrano, M. ; Ma, Jian Feng ; Kochian, Leon V. / Root and shoot transcriptome analysis of two ecotypes of Noccaea caerulescens uncovers the role of NcNramp1 in Cd hyperaccumulation. In: Plant Journal. 2014 ; Vol. 78, No. 3. pp. 398-410.
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