Roles of EDNs in regulating oviductal NO synthesis and smooth muscle motility in cows

Yoshihiko Kobayashi, Yuka Yoshimoto, Yuki Yamamoto, Koji Kimura, Kiyoshi Okuda

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Endothelins (EDNs) participate in various physiological events including smooth muscle contraction, nitric oxide (NO) synthesis, and embryonic development. In this study, we investigated the regional roles of EDNs produced by bovine oviductal epithelial cells in NO synthesis and smooth muscle motility. Quantification of mRNA expressions indicated that expression of EDN receptor B (EDNRB) in the ampullary region was higher after ovulation than before ovulation, whereas expression of EDNRA in the isthmic region was higher after ovulation than before ovulation. Immunohistochemistry revealed that the EDN receptors (EDNRA and EDNRB) were expressed in the epithelium, whereas smooth muscle showed positive staining only for EDNRA. The expressions of inducible NO synthase (iNOS) protein and its mRNA (NOS2) in cultured epithelial cells isolated from the ampulla were stimulated by EDN1, but not by EDN2 or EDN3, after 1 h of incubation. In isthmic epithelial cells, none of the EDNs affected the expression of NOS2. Isometric contraction tests indicated that spontaneous waves were strong in the isthmic region but weak in the ampullary region. EDN1 modulated smooth muscle motility in both the regions. The overall findings suggest that EDN1 plays region-specific roles in smooth muscle motility and epithelial NO synthesis, providing an optimal oviductal microenvironment for transport of gametes, fertilization, and development/transport of early embryo.

Original languageEnglish
Pages (from-to)615-622
Number of pages8
JournalReproduction
Volume151
Issue number6
DOIs
Publication statusPublished - Jun 1 2016

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Endothelins
Smooth Muscle
Nitric Oxide
Ovulation
Epithelial Cells
Endothelin B Receptors
Endothelin Receptors
Messenger RNA
Isometric Contraction
Nitric Oxide Synthase Type II
Muscle Contraction
Fertilization
Germ Cells
Embryonic Development
Cultured Cells
Embryonic Structures
Epithelium
Immunohistochemistry
Staining and Labeling
Proteins

ASJC Scopus subject areas

  • Obstetrics and Gynaecology
  • Reproductive Medicine
  • Embryology
  • Endocrinology
  • Cell Biology

Cite this

Roles of EDNs in regulating oviductal NO synthesis and smooth muscle motility in cows. / Kobayashi, Yoshihiko; Yoshimoto, Yuka; Yamamoto, Yuki; Kimura, Koji; Okuda, Kiyoshi.

In: Reproduction, Vol. 151, No. 6, 01.06.2016, p. 615-622.

Research output: Contribution to journalArticle

Kobayashi, Yoshihiko ; Yoshimoto, Yuka ; Yamamoto, Yuki ; Kimura, Koji ; Okuda, Kiyoshi. / Roles of EDNs in regulating oviductal NO synthesis and smooth muscle motility in cows. In: Reproduction. 2016 ; Vol. 151, No. 6. pp. 615-622.
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