Roles of carbohydrates on Cry j 1, the major allergen of Japanese cedar pollen, in specific T-cell responses

Mitsuhiro Okano, Kohsuke Kino, Teruaki Takishita, Hisashi Hattori, Teruhiro Ogawa, Tadashi Yoshino, Minehiko Yokoyama, Kazunori Nishizaki

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Background: Carbohydrates expressed on allergens are known to be important for allergenicity. However, little is known about whether the carbohydrates drive the TH2 response. Objective: We sought to determine a role for carbohydrates expressed on Cry j 1, which is the major allergen of Cryptomeria japonica pollen and causes the most prevalent pollinosis in Japan, in in vitro cellular responses. Methods: Carbohydrates on Cry j 1 were destroyed by periodate-oxidation under mild conditions. Proliferative responses and cytokine productions against native, periodate-treated, and mock-treated Cry j 1 were compared in peripheral blood mononuclear cells, Cry j 1-specific T-cell lines, and clones from patients with Japanese cedar pollinosis. Results: We found that peripheral blood mononuclear cells from patients with Japanese cedar pollinosis displayed a significant decrease in proliferation and IL-5 production in response to periodate-treated Cry j 1 in comparison with native and mock-treated Cry j 1. Decreased proliferative responses against periodate-treated Cry j 1 were also seen in polyclonal T-cell lines, and the responses showed a heterogeneity. In addition, Cry j 1-specific CD4+ T-cell clones also displayed a significant decrease in proliferation and IL-4 and IL-5 production - but not IFN-γ production - in comparison with the control antigens. However, most of the clones showed decreased but positive proliferative responses against periodate-treated Cry j 1. Blockade of the mannose receptor had no effect on cellular responses. Conclusion: The results suggest that carbohydrates on Cry j 1 play a major role in promoting Cry j 1-specific TH2 response in vitro, though they are not major targets as T-cell epitopes.

Original languageEnglish
Pages (from-to)101-108
Number of pages8
JournalJournal of Allergy and Clinical Immunology
Volume108
Issue number1
DOIs
Publication statusPublished - 2001

Fingerprint

Cryptomeria
Pollen
Allergens
Carbohydrates
Seasonal Allergic Rhinitis
T-Lymphocytes
Clone Cells
Interleukin-5
Blood Cells
Cell Line
T-Lymphocyte Epitopes
Interleukin-4
Japan
metaperiodate
Cytokines
Antigens

Keywords

  • Allergen
  • Carbohydrate
  • Clones
  • Cry j 1
  • T cell
  • T2, mannose receptor

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Roles of carbohydrates on Cry j 1, the major allergen of Japanese cedar pollen, in specific T-cell responses. / Okano, Mitsuhiro; Kino, Kohsuke; Takishita, Teruaki; Hattori, Hisashi; Ogawa, Teruhiro; Yoshino, Tadashi; Yokoyama, Minehiko; Nishizaki, Kazunori.

In: Journal of Allergy and Clinical Immunology, Vol. 108, No. 1, 2001, p. 101-108.

Research output: Contribution to journalArticle

Okano, Mitsuhiro ; Kino, Kohsuke ; Takishita, Teruaki ; Hattori, Hisashi ; Ogawa, Teruhiro ; Yoshino, Tadashi ; Yokoyama, Minehiko ; Nishizaki, Kazunori. / Roles of carbohydrates on Cry j 1, the major allergen of Japanese cedar pollen, in specific T-cell responses. In: Journal of Allergy and Clinical Immunology. 2001 ; Vol. 108, No. 1. pp. 101-108.
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abstract = "Background: Carbohydrates expressed on allergens are known to be important for allergenicity. However, little is known about whether the carbohydrates drive the TH2 response. Objective: We sought to determine a role for carbohydrates expressed on Cry j 1, which is the major allergen of Cryptomeria japonica pollen and causes the most prevalent pollinosis in Japan, in in vitro cellular responses. Methods: Carbohydrates on Cry j 1 were destroyed by periodate-oxidation under mild conditions. Proliferative responses and cytokine productions against native, periodate-treated, and mock-treated Cry j 1 were compared in peripheral blood mononuclear cells, Cry j 1-specific T-cell lines, and clones from patients with Japanese cedar pollinosis. Results: We found that peripheral blood mononuclear cells from patients with Japanese cedar pollinosis displayed a significant decrease in proliferation and IL-5 production in response to periodate-treated Cry j 1 in comparison with native and mock-treated Cry j 1. Decreased proliferative responses against periodate-treated Cry j 1 were also seen in polyclonal T-cell lines, and the responses showed a heterogeneity. In addition, Cry j 1-specific CD4+ T-cell clones also displayed a significant decrease in proliferation and IL-4 and IL-5 production - but not IFN-γ production - in comparison with the control antigens. However, most of the clones showed decreased but positive proliferative responses against periodate-treated Cry j 1. Blockade of the mannose receptor had no effect on cellular responses. Conclusion: The results suggest that carbohydrates on Cry j 1 play a major role in promoting Cry j 1-specific TH2 response in vitro, though they are not major targets as T-cell epitopes.",
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AU - Okano, Mitsuhiro

AU - Kino, Kohsuke

AU - Takishita, Teruaki

AU - Hattori, Hisashi

AU - Ogawa, Teruhiro

AU - Yoshino, Tadashi

AU - Yokoyama, Minehiko

AU - Nishizaki, Kazunori

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N2 - Background: Carbohydrates expressed on allergens are known to be important for allergenicity. However, little is known about whether the carbohydrates drive the TH2 response. Objective: We sought to determine a role for carbohydrates expressed on Cry j 1, which is the major allergen of Cryptomeria japonica pollen and causes the most prevalent pollinosis in Japan, in in vitro cellular responses. Methods: Carbohydrates on Cry j 1 were destroyed by periodate-oxidation under mild conditions. Proliferative responses and cytokine productions against native, periodate-treated, and mock-treated Cry j 1 were compared in peripheral blood mononuclear cells, Cry j 1-specific T-cell lines, and clones from patients with Japanese cedar pollinosis. Results: We found that peripheral blood mononuclear cells from patients with Japanese cedar pollinosis displayed a significant decrease in proliferation and IL-5 production in response to periodate-treated Cry j 1 in comparison with native and mock-treated Cry j 1. Decreased proliferative responses against periodate-treated Cry j 1 were also seen in polyclonal T-cell lines, and the responses showed a heterogeneity. In addition, Cry j 1-specific CD4+ T-cell clones also displayed a significant decrease in proliferation and IL-4 and IL-5 production - but not IFN-γ production - in comparison with the control antigens. However, most of the clones showed decreased but positive proliferative responses against periodate-treated Cry j 1. Blockade of the mannose receptor had no effect on cellular responses. Conclusion: The results suggest that carbohydrates on Cry j 1 play a major role in promoting Cry j 1-specific TH2 response in vitro, though they are not major targets as T-cell epitopes.

AB - Background: Carbohydrates expressed on allergens are known to be important for allergenicity. However, little is known about whether the carbohydrates drive the TH2 response. Objective: We sought to determine a role for carbohydrates expressed on Cry j 1, which is the major allergen of Cryptomeria japonica pollen and causes the most prevalent pollinosis in Japan, in in vitro cellular responses. Methods: Carbohydrates on Cry j 1 were destroyed by periodate-oxidation under mild conditions. Proliferative responses and cytokine productions against native, periodate-treated, and mock-treated Cry j 1 were compared in peripheral blood mononuclear cells, Cry j 1-specific T-cell lines, and clones from patients with Japanese cedar pollinosis. Results: We found that peripheral blood mononuclear cells from patients with Japanese cedar pollinosis displayed a significant decrease in proliferation and IL-5 production in response to periodate-treated Cry j 1 in comparison with native and mock-treated Cry j 1. Decreased proliferative responses against periodate-treated Cry j 1 were also seen in polyclonal T-cell lines, and the responses showed a heterogeneity. In addition, Cry j 1-specific CD4+ T-cell clones also displayed a significant decrease in proliferation and IL-4 and IL-5 production - but not IFN-γ production - in comparison with the control antigens. However, most of the clones showed decreased but positive proliferative responses against periodate-treated Cry j 1. Blockade of the mannose receptor had no effect on cellular responses. Conclusion: The results suggest that carbohydrates on Cry j 1 play a major role in promoting Cry j 1-specific TH2 response in vitro, though they are not major targets as T-cell epitopes.

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KW - Clones

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KW - T cell

KW - T2, mannose receptor

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