Roles of AtTPC1, vacuolar two pore channel 1, in arabidopsis stomatal closure

Mohammad Mahbub Islam, Shintaro Munemasa, Mohammad Anowar Hossain, Yoshimasa Nakamura, Izumi Mori, Yoshiyuki Murata

Research output: Contribution to journalArticle

60 Citations (Scopus)

Abstract

Abscisic acid (ABA) induces production of reactive oxygen species (ROS) and nitric oxide (NO), elevation of the cytosolic free calcium ion concentration ([Ca2]cyt) and cytosolic pH (pHcyt), and activation of S-type anion channels in guard cells, causing stomatal closure. To investigate whether Arabidopsis Two pore channel 1 (AtTPC1) that encodes the slow vacuolar (SV) channel is involved in stomatal closure, we examined stomatal movements and mobilization of second messengers in the attpc1-2 loss-of-function mutant in response to ABA, methyl jasmonate (MeJA) and Ca2. Both ABA and MeJA elicited production of ROS and NO, [Ca2]cyt oscillations, cytosolic alkalization and activation of S-type anion channel currents to lead to stomatal closure in the attpc1-2 mutant as well as the wild type. Unlike the wild type, in the attpc1-2 mutant exogenous Ca2 neither induced stomatal closure nor activated plasma membrane S-type anion channel currents despite [Ca 2]cyt elevation. These results indicate that AtTPC1 functions in response to external Ca2 but not to ABA and MeJA in Arabidopsis guard cells and suggest that AtTPC1 could be involved in priming of plasma membrane S-type anion channels by external Ca2 in Arabidopsis guard cells.

Original languageEnglish
Pages (from-to)302-311
Number of pages10
JournalPlant and Cell Physiology
Volume51
Issue number2
DOIs
Publication statusPublished - Feb 2010

Fingerprint

Abscisic Acid
Arabidopsis
Anions
calcium
anions
abscisic acid
methyl jasmonate
guard cells
Reactive Oxygen Species
Nitric Oxide
Cell Membrane
Second Messenger Systems
mutants
nitric oxide
reactive oxygen species
plasma membrane
stomatal movement
alkalinization
Ions
Calcium

Keywords

  • Abscisic acid
  • Calcium
  • Cytosolic free calcium
  • Cytosolic pH
  • Methyl jasmonate
  • S-type anion channel

ASJC Scopus subject areas

  • Plant Science
  • Physiology
  • Cell Biology

Cite this

Roles of AtTPC1, vacuolar two pore channel 1, in arabidopsis stomatal closure. / Islam, Mohammad Mahbub; Munemasa, Shintaro; Hossain, Mohammad Anowar; Nakamura, Yoshimasa; Mori, Izumi; Murata, Yoshiyuki.

In: Plant and Cell Physiology, Vol. 51, No. 2, 02.2010, p. 302-311.

Research output: Contribution to journalArticle

@article{fadf05e7c6f44ffbacf6ba6c0f731fc5,
title = "Roles of AtTPC1, vacuolar two pore channel 1, in arabidopsis stomatal closure",
abstract = "Abscisic acid (ABA) induces production of reactive oxygen species (ROS) and nitric oxide (NO), elevation of the cytosolic free calcium ion concentration ([Ca2]cyt) and cytosolic pH (pHcyt), and activation of S-type anion channels in guard cells, causing stomatal closure. To investigate whether Arabidopsis Two pore channel 1 (AtTPC1) that encodes the slow vacuolar (SV) channel is involved in stomatal closure, we examined stomatal movements and mobilization of second messengers in the attpc1-2 loss-of-function mutant in response to ABA, methyl jasmonate (MeJA) and Ca2. Both ABA and MeJA elicited production of ROS and NO, [Ca2]cyt oscillations, cytosolic alkalization and activation of S-type anion channel currents to lead to stomatal closure in the attpc1-2 mutant as well as the wild type. Unlike the wild type, in the attpc1-2 mutant exogenous Ca2 neither induced stomatal closure nor activated plasma membrane S-type anion channel currents despite [Ca 2]cyt elevation. These results indicate that AtTPC1 functions in response to external Ca2 but not to ABA and MeJA in Arabidopsis guard cells and suggest that AtTPC1 could be involved in priming of plasma membrane S-type anion channels by external Ca2 in Arabidopsis guard cells.",
keywords = "Abscisic acid, Calcium, Cytosolic free calcium, Cytosolic pH, Methyl jasmonate, S-type anion channel",
author = "Islam, {Mohammad Mahbub} and Shintaro Munemasa and Hossain, {Mohammad Anowar} and Yoshimasa Nakamura and Izumi Mori and Yoshiyuki Murata",
year = "2010",
month = "2",
doi = "10.1093/pcp/pcq001",
language = "English",
volume = "51",
pages = "302--311",
journal = "Plant and Cell Physiology",
issn = "0032-0781",
publisher = "Oxford University Press",
number = "2",

}

TY - JOUR

T1 - Roles of AtTPC1, vacuolar two pore channel 1, in arabidopsis stomatal closure

AU - Islam, Mohammad Mahbub

AU - Munemasa, Shintaro

AU - Hossain, Mohammad Anowar

AU - Nakamura, Yoshimasa

AU - Mori, Izumi

AU - Murata, Yoshiyuki

PY - 2010/2

Y1 - 2010/2

N2 - Abscisic acid (ABA) induces production of reactive oxygen species (ROS) and nitric oxide (NO), elevation of the cytosolic free calcium ion concentration ([Ca2]cyt) and cytosolic pH (pHcyt), and activation of S-type anion channels in guard cells, causing stomatal closure. To investigate whether Arabidopsis Two pore channel 1 (AtTPC1) that encodes the slow vacuolar (SV) channel is involved in stomatal closure, we examined stomatal movements and mobilization of second messengers in the attpc1-2 loss-of-function mutant in response to ABA, methyl jasmonate (MeJA) and Ca2. Both ABA and MeJA elicited production of ROS and NO, [Ca2]cyt oscillations, cytosolic alkalization and activation of S-type anion channel currents to lead to stomatal closure in the attpc1-2 mutant as well as the wild type. Unlike the wild type, in the attpc1-2 mutant exogenous Ca2 neither induced stomatal closure nor activated plasma membrane S-type anion channel currents despite [Ca 2]cyt elevation. These results indicate that AtTPC1 functions in response to external Ca2 but not to ABA and MeJA in Arabidopsis guard cells and suggest that AtTPC1 could be involved in priming of plasma membrane S-type anion channels by external Ca2 in Arabidopsis guard cells.

AB - Abscisic acid (ABA) induces production of reactive oxygen species (ROS) and nitric oxide (NO), elevation of the cytosolic free calcium ion concentration ([Ca2]cyt) and cytosolic pH (pHcyt), and activation of S-type anion channels in guard cells, causing stomatal closure. To investigate whether Arabidopsis Two pore channel 1 (AtTPC1) that encodes the slow vacuolar (SV) channel is involved in stomatal closure, we examined stomatal movements and mobilization of second messengers in the attpc1-2 loss-of-function mutant in response to ABA, methyl jasmonate (MeJA) and Ca2. Both ABA and MeJA elicited production of ROS and NO, [Ca2]cyt oscillations, cytosolic alkalization and activation of S-type anion channel currents to lead to stomatal closure in the attpc1-2 mutant as well as the wild type. Unlike the wild type, in the attpc1-2 mutant exogenous Ca2 neither induced stomatal closure nor activated plasma membrane S-type anion channel currents despite [Ca 2]cyt elevation. These results indicate that AtTPC1 functions in response to external Ca2 but not to ABA and MeJA in Arabidopsis guard cells and suggest that AtTPC1 could be involved in priming of plasma membrane S-type anion channels by external Ca2 in Arabidopsis guard cells.

KW - Abscisic acid

KW - Calcium

KW - Cytosolic free calcium

KW - Cytosolic pH

KW - Methyl jasmonate

KW - S-type anion channel

UR - http://www.scopus.com/inward/record.url?scp=76749133617&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=76749133617&partnerID=8YFLogxK

U2 - 10.1093/pcp/pcq001

DO - 10.1093/pcp/pcq001

M3 - Article

VL - 51

SP - 302

EP - 311

JO - Plant and Cell Physiology

JF - Plant and Cell Physiology

SN - 0032-0781

IS - 2

ER -