Roles of Arg- and Lys-gingipains in coaggregation of Porphyromonas gingivalis

Identification of its responsible molecules in translation products of rgpA, kgp, and hagA genes

Naoko Abe, Atsuyo Baba, Ryosuke Takii, Koji Nakayama, Arihide Kamaguchi, Yasuko Shibata, Yoshimitsu Abiko, Kuniaki Okamoto, Tomoko Kadowaki, Kenji Yamamoto

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Arg- (Rgp) and Lys-gingipains (Kgp) are two individual cysteine proteinases produced by Porphyromonas gingivalis, an oral anaerobic bacterium, and are implicated as major virulence factors in a wide range of pathologies of adult periodontitis. Coaggregation of this bacterium with other oral bacteria is an initial and critical step in infectious processes, yet the factors and mechanisms responsible for this process remain elusive. Here we show that the initial translation products of the rgpA, kgp and hemagglutinin hagA genes are responsible for coaggregation of P. gingivalis and that the proteolytic activity of Rgp and Kgp is indispensable in this process. The rgpA rgpB kgp- and rgpA kgp hagA-deficient triple mutants exhibited no coaggregation activity with Actinomyces viscosus, whereas the kgp-null and rgpA rgpB-deficient double mutants significantly retained this activity. Consistently, the combined action of Rgp- and Kgp-specific inhibitors strongly inhibited the coaggregation activity of the bacterium, although single use of Rgp- or Kgp-specific inhibitor significantly retained this activity. We also demonstrate that the 47- and 43-kDa proteins produced from the translation products of the rgpA, kgp, and hagA genes by proteolytic activity of both Rgp and Kgp are responsible for the coaggregation of P. gingivalis.

Original languageEnglish
Pages (from-to)1041-1047
Number of pages7
JournalBiological Chemistry
Volume385
Issue number11
DOIs
Publication statusPublished - Nov 2004
Externally publishedYes

Fingerprint

Porphyromonas gingivalis
Bacteria
Genes
Molecules
Actinomyces viscosus
Chronic Periodontitis
Cysteine Proteases
Anaerobic Bacteria
Hemagglutinins
Protein Biosynthesis
Virulence Factors
Pathology
Lys-gingipain
Proteins

Keywords

  • Arg-gingipain
  • Coaggregation
  • Cysteine proteinase
  • Lys-gingipain
  • Periodontal disease
  • Porphyromonas gingivalis

ASJC Scopus subject areas

  • Biochemistry

Cite this

Roles of Arg- and Lys-gingipains in coaggregation of Porphyromonas gingivalis : Identification of its responsible molecules in translation products of rgpA, kgp, and hagA genes. / Abe, Naoko; Baba, Atsuyo; Takii, Ryosuke; Nakayama, Koji; Kamaguchi, Arihide; Shibata, Yasuko; Abiko, Yoshimitsu; Okamoto, Kuniaki; Kadowaki, Tomoko; Yamamoto, Kenji.

In: Biological Chemistry, Vol. 385, No. 11, 11.2004, p. 1041-1047.

Research output: Contribution to journalArticle

Abe, Naoko ; Baba, Atsuyo ; Takii, Ryosuke ; Nakayama, Koji ; Kamaguchi, Arihide ; Shibata, Yasuko ; Abiko, Yoshimitsu ; Okamoto, Kuniaki ; Kadowaki, Tomoko ; Yamamoto, Kenji. / Roles of Arg- and Lys-gingipains in coaggregation of Porphyromonas gingivalis : Identification of its responsible molecules in translation products of rgpA, kgp, and hagA genes. In: Biological Chemistry. 2004 ; Vol. 385, No. 11. pp. 1041-1047.
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AU - Kadowaki, Tomoko

AU - Yamamoto, Kenji

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