Role of Vibrio mimicus protease in enterotoxigenicity.

M. A. Chowdhury, Shin-ichi Miyoshi, S. Shinoda

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Recently, we have purified and characterised a metalloprotease produced by Vibrio mimicus. The role of V. mimicus protease (VMP) in fluid accumulation (FA) in rabbit ileal loops (RIL) was investigated. Although live cells or crude VMP prepared from cell-free culture supernatant showed activity in RIL, the purified VMP failed to induce FA in the RIL. When VMP in crude preparation was inactivated by preparing the fraction in the presence of EGTA (Ethyleneglycol-bis [beta-aminoethylether]-N, N, N1, N1,-tetraacetic acid), a chelating agent for Ca2+ (EGTA-crude), or treatment with phosphoramidon (a metalloprotease inhibitor), the preparation became negative in RIL assay. Again, the EGTA-crude induced FA in RIL when retreated with 2mM CaCl2 after elimination of EGTA. The results show evidences of attribution of VMP in RIL activity. These observations, therefore, suggest that the protease produced by V. mimicus may play a potential role in enterotoxigenicity.

Original languageEnglish
Pages (from-to)332-334
Number of pages3
JournalJournal of Diarrhoeal Diseases Research
Volume9
Issue number4
Publication statusPublished - Dec 1991

Fingerprint

Vibrio mimicus
Peptide Hydrolases
proteinases
Egtazic Acid
Rabbits
rabbits
Metalloproteases
metalloproteinases
Chelating Agents
chelating agents
Cell Culture Techniques
cells
Acids
calcium

ASJC Scopus subject areas

  • Gastroenterology

Cite this

Role of Vibrio mimicus protease in enterotoxigenicity. / Chowdhury, M. A.; Miyoshi, Shin-ichi; Shinoda, S.

In: Journal of Diarrhoeal Diseases Research, Vol. 9, No. 4, 12.1991, p. 332-334.

Research output: Contribution to journalArticle

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