Role of trehalose synthesis in ralstonia syzygii subsp. Indonesiensis PW1001 in inducing hypersensitive response on eggplant (solanum melongena cv. senryo-nigou)

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Abstract

Ralstonia syzygii subsp. indonesiensis (Rsi, former name: Ralstonia solanacearum phylotype IV) PW1001, a causal agent of potato wilt disease, induces hypersensitive response (HR) on its non-host eggplant (Solanum melongena cv. Senryo-nigou). The disaccharide treha-lose is involved in abiotic and biotic stress tolerance in many organisms. We found that trehalose is required for eliciting HR on eggplant by plant pathogen Rsi PW1001. In R. solanacearum, it is known that the OtsA/ OtsB pathway is the dominant trehalose synthesis path-way, and otsA and otsB encode trehalose-6-phosphate (T6P) synthase and T6P phosphatase, respectively. We generated otsA and otsB mutant strains and found that these mutant strains reduced the bacterial trehalose concentration and HR induction on eggplant leaves compared to wild-type. Trehalose functions intracellu-larly in Rsi PW1001 because addition of exogenous tre-halose did not affect the HR level and ion leakage. Re-quirement of trehalose in HR induction is not common in R. solanacearum species complex because mutation of otsA in Ralstonia pseudosolanacearum (former name: Ralstonia solanacearum phylotype I) RS1002 did not affect HR on the leaves of its non-host tobacco and wild eggplant Solanum torvum. Further, we also found that each otsA and otsB mutant had reduced ability to grow in a medium containing NaCl and sucrose, indicating that trehalose also has an important role in osmotic stress tolerance.

Original languageEnglish
Pages (from-to)566-579
Number of pages14
JournalPlant Pathology Journal
Volume37
Issue number6
DOIs
Publication statusPublished - Dec 2021

Keywords

  • Eggplant
  • Hypersensitive response
  • Ralstonia solanacearum species complex
  • Trehalose

ASJC Scopus subject areas

  • Agronomy and Crop Science

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