Role of the transcription factor Sp1 in regulating the expression of the murine cathepsin e gene

Kuniaki Okamoto, Yoshiko Okamoto, Tomoyo Kawakubo, Jun Ichi Iwata, Yoshiyuki Yasuda, Takayuki Tsukuba, Kenji Yamamoto

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Cathepsin E (CE) is an intracellular aspartic proteinase that is exclusively expressed in cells of the gastrointestinal tracts, lymphoid tissues, urinary organs and red blood cells. However, the molecular mechanism by which CE is predominantly expressed in these cells remains unknown. Here, we report the identification of several transcription start sites of the CE gene and their regulatory factors in gastric adenosarcoma cells. We first identified several unique transcription start sites in mouse CE genes by an oligo cap method. Their analysis also revealed the existence of a non-coding region ∼24-kb upstream of exon 1 in the CE gene and also the existence of two transcripts for CE. Luciferase analyses in upstream of exon 1 revealed that this site contained putative binding regions for the transcription factors Sp1, AP-1 and cEts-1 essential for the expression of CE gene. Moreover, electrophoretic mobility shift assays revealed that the protein-oligonucleotides complex of the Sp1 site were supershifted by an anti-Sp1 antibody. The chromatin immunoprecipitation assay showed that Sp1 bound to the CE promoter region. In addition, overexpression of the Sp1 protein increased the expression of the CE protein. Altogether, these results suggest that Sp1 binding plays a particularly important role in the regulation of CE gene expression.

Original languageEnglish
Pages (from-to)263-272
Number of pages10
JournalJournal of Biochemistry
Volume151
Issue number3
DOIs
Publication statusPublished - Mar 2012
Externally publishedYes

Fingerprint

Cathepsin E
Sp1 Transcription Factor
Cathepsins
Genes
Transcription Initiation Site
Exons
Assays
Adenosarcoma
Aspartic Acid Proteases
Electrophoretic mobility
Proteins
Chromatin Immunoprecipitation
Transcription Factor AP-1
Electrophoretic Mobility Shift Assay
Lymphoid Tissue
Regulator Genes
Luciferases
Genetic Promoter Regions
Gene expression
Oligonucleotides

Keywords

  • aspartic proteinase
  • cathepsin E
  • promoter
  • Sp1

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

Cite this

Okamoto, K., Okamoto, Y., Kawakubo, T., Iwata, J. I., Yasuda, Y., Tsukuba, T., & Yamamoto, K. (2012). Role of the transcription factor Sp1 in regulating the expression of the murine cathepsin e gene. Journal of Biochemistry, 151(3), 263-272. https://doi.org/10.1093/jb/mvr135

Role of the transcription factor Sp1 in regulating the expression of the murine cathepsin e gene. / Okamoto, Kuniaki; Okamoto, Yoshiko; Kawakubo, Tomoyo; Iwata, Jun Ichi; Yasuda, Yoshiyuki; Tsukuba, Takayuki; Yamamoto, Kenji.

In: Journal of Biochemistry, Vol. 151, No. 3, 03.2012, p. 263-272.

Research output: Contribution to journalArticle

Okamoto, K, Okamoto, Y, Kawakubo, T, Iwata, JI, Yasuda, Y, Tsukuba, T & Yamamoto, K 2012, 'Role of the transcription factor Sp1 in regulating the expression of the murine cathepsin e gene', Journal of Biochemistry, vol. 151, no. 3, pp. 263-272. https://doi.org/10.1093/jb/mvr135
Okamoto, Kuniaki ; Okamoto, Yoshiko ; Kawakubo, Tomoyo ; Iwata, Jun Ichi ; Yasuda, Yoshiyuki ; Tsukuba, Takayuki ; Yamamoto, Kenji. / Role of the transcription factor Sp1 in regulating the expression of the murine cathepsin e gene. In: Journal of Biochemistry. 2012 ; Vol. 151, No. 3. pp. 263-272.
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