Role of the mannose receptor in phagocytosis of Enterococcus faecalis strain EC-12 by antigen-presenting cells

Takeshi Tsuruta, Ryo Inoue, Takayuki Nagino, Ryoichiro Nishibayashi, Yuko Makioka, Kazunari Ushida

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

The aim of this study was to clarify the phagocytic mechanisms of a heat-killed cell preparation of Enterococcus faecalis strain EC-12 (EC-12) by antigen-presenting cells (APCs). Fluorescein isothiocyanate (FITC)-labeled EC-12 was cocultured with peritoneal macrophage and the amount of EC-12 phagocytosed by peritoneal macrophages was measured using a microplate fluorometer. Peritoneal macrophages from toll-like receptor (TLR)2-, TLR7-, and MyD88-deficient knockout (KO) mice exhibited similar levels of EC-12 phagocytosis to those from wild-type mice. Similarly, dectin-1 neutralization of peritoneal macrophages had no effect on EC-12 phagocytosis. However, blockade of the mannose receptor (MR) significantly decreased the amount of EC-12 phagocytosed by peritoneal macrophages; the same effect was observed in bone marrow-derived macrophages and dendritic cells. Our findings suggest that MR plays a major role in EC-12 phagocytosis by the APCs. This aim of this study was to clarify the phagocytic mechanisms of a heat-killed cell preparation of Enterococcus faecalis strain EC-12 (EC-12) by antigen-presenting cells (APCs). Our findings suggest that mannose receptor (MR) plays a major role in EC-12 phagocytosis by the APCs.

Original languageEnglish
Pages (from-to)610-617
Number of pages8
JournalMicrobiologyOpen
Volume2
Issue number4
DOIs
Publication statusPublished - Aug 2013
Externally publishedYes

Fingerprint

Enterococcus faecalis
Antigen-Presenting Cells
Phagocytosis
Peritoneal Macrophages
Hot Temperature
Toll-Like Receptor 2
Fluorescein
Knockout Mice
Dendritic Cells
mannose receptor
Macrophages

Keywords

  • Antigen-presenting cell
  • EC-12
  • LAB
  • Macrophage
  • Mannose receptor
  • Phagocytosis

ASJC Scopus subject areas

  • Microbiology

Cite this

Role of the mannose receptor in phagocytosis of Enterococcus faecalis strain EC-12 by antigen-presenting cells. / Tsuruta, Takeshi; Inoue, Ryo; Nagino, Takayuki; Nishibayashi, Ryoichiro; Makioka, Yuko; Ushida, Kazunari.

In: MicrobiologyOpen, Vol. 2, No. 4, 08.2013, p. 610-617.

Research output: Contribution to journalArticle

Tsuruta, Takeshi ; Inoue, Ryo ; Nagino, Takayuki ; Nishibayashi, Ryoichiro ; Makioka, Yuko ; Ushida, Kazunari. / Role of the mannose receptor in phagocytosis of Enterococcus faecalis strain EC-12 by antigen-presenting cells. In: MicrobiologyOpen. 2013 ; Vol. 2, No. 4. pp. 610-617.
@article{5eca6dddc4d946558a8cbe796f5a383f,
title = "Role of the mannose receptor in phagocytosis of Enterococcus faecalis strain EC-12 by antigen-presenting cells",
abstract = "The aim of this study was to clarify the phagocytic mechanisms of a heat-killed cell preparation of Enterococcus faecalis strain EC-12 (EC-12) by antigen-presenting cells (APCs). Fluorescein isothiocyanate (FITC)-labeled EC-12 was cocultured with peritoneal macrophage and the amount of EC-12 phagocytosed by peritoneal macrophages was measured using a microplate fluorometer. Peritoneal macrophages from toll-like receptor (TLR)2-, TLR7-, and MyD88-deficient knockout (KO) mice exhibited similar levels of EC-12 phagocytosis to those from wild-type mice. Similarly, dectin-1 neutralization of peritoneal macrophages had no effect on EC-12 phagocytosis. However, blockade of the mannose receptor (MR) significantly decreased the amount of EC-12 phagocytosed by peritoneal macrophages; the same effect was observed in bone marrow-derived macrophages and dendritic cells. Our findings suggest that MR plays a major role in EC-12 phagocytosis by the APCs. This aim of this study was to clarify the phagocytic mechanisms of a heat-killed cell preparation of Enterococcus faecalis strain EC-12 (EC-12) by antigen-presenting cells (APCs). Our findings suggest that mannose receptor (MR) plays a major role in EC-12 phagocytosis by the APCs.",
keywords = "Antigen-presenting cell, EC-12, LAB, Macrophage, Mannose receptor, Phagocytosis",
author = "Takeshi Tsuruta and Ryo Inoue and Takayuki Nagino and Ryoichiro Nishibayashi and Yuko Makioka and Kazunari Ushida",
year = "2013",
month = "8",
doi = "10.1002/mbo3.99",
language = "English",
volume = "2",
pages = "610--617",
journal = "MicrobiologyOpen",
issn = "2045-8827",
publisher = "John Wiley and Sons Inc.",
number = "4",

}

TY - JOUR

T1 - Role of the mannose receptor in phagocytosis of Enterococcus faecalis strain EC-12 by antigen-presenting cells

AU - Tsuruta, Takeshi

AU - Inoue, Ryo

AU - Nagino, Takayuki

AU - Nishibayashi, Ryoichiro

AU - Makioka, Yuko

AU - Ushida, Kazunari

PY - 2013/8

Y1 - 2013/8

N2 - The aim of this study was to clarify the phagocytic mechanisms of a heat-killed cell preparation of Enterococcus faecalis strain EC-12 (EC-12) by antigen-presenting cells (APCs). Fluorescein isothiocyanate (FITC)-labeled EC-12 was cocultured with peritoneal macrophage and the amount of EC-12 phagocytosed by peritoneal macrophages was measured using a microplate fluorometer. Peritoneal macrophages from toll-like receptor (TLR)2-, TLR7-, and MyD88-deficient knockout (KO) mice exhibited similar levels of EC-12 phagocytosis to those from wild-type mice. Similarly, dectin-1 neutralization of peritoneal macrophages had no effect on EC-12 phagocytosis. However, blockade of the mannose receptor (MR) significantly decreased the amount of EC-12 phagocytosed by peritoneal macrophages; the same effect was observed in bone marrow-derived macrophages and dendritic cells. Our findings suggest that MR plays a major role in EC-12 phagocytosis by the APCs. This aim of this study was to clarify the phagocytic mechanisms of a heat-killed cell preparation of Enterococcus faecalis strain EC-12 (EC-12) by antigen-presenting cells (APCs). Our findings suggest that mannose receptor (MR) plays a major role in EC-12 phagocytosis by the APCs.

AB - The aim of this study was to clarify the phagocytic mechanisms of a heat-killed cell preparation of Enterococcus faecalis strain EC-12 (EC-12) by antigen-presenting cells (APCs). Fluorescein isothiocyanate (FITC)-labeled EC-12 was cocultured with peritoneal macrophage and the amount of EC-12 phagocytosed by peritoneal macrophages was measured using a microplate fluorometer. Peritoneal macrophages from toll-like receptor (TLR)2-, TLR7-, and MyD88-deficient knockout (KO) mice exhibited similar levels of EC-12 phagocytosis to those from wild-type mice. Similarly, dectin-1 neutralization of peritoneal macrophages had no effect on EC-12 phagocytosis. However, blockade of the mannose receptor (MR) significantly decreased the amount of EC-12 phagocytosed by peritoneal macrophages; the same effect was observed in bone marrow-derived macrophages and dendritic cells. Our findings suggest that MR plays a major role in EC-12 phagocytosis by the APCs. This aim of this study was to clarify the phagocytic mechanisms of a heat-killed cell preparation of Enterococcus faecalis strain EC-12 (EC-12) by antigen-presenting cells (APCs). Our findings suggest that mannose receptor (MR) plays a major role in EC-12 phagocytosis by the APCs.

KW - Antigen-presenting cell

KW - EC-12

KW - LAB

KW - Macrophage

KW - Mannose receptor

KW - Phagocytosis

UR - http://www.scopus.com/inward/record.url?scp=84897107647&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84897107647&partnerID=8YFLogxK

U2 - 10.1002/mbo3.99

DO - 10.1002/mbo3.99

M3 - Article

C2 - 23801521

AN - SCOPUS:84897107647

VL - 2

SP - 610

EP - 617

JO - MicrobiologyOpen

JF - MicrobiologyOpen

SN - 2045-8827

IS - 4

ER -