Role of the mannose receptor in phagocytosis of Enterococcus faecalis strain EC-12 by antigen-presenting cells

Takeshi Tsuruta, Ryo Inoue, Takayuki Nagino, Ryoichiro Nishibayashi, Yuko Makioka, Kazunari Ushida

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

The aim of this study was to clarify the phagocytic mechanisms of a heat-killed cell preparation of Enterococcus faecalis strain EC-12 (EC-12) by antigen-presenting cells (APCs). Fluorescein isothiocyanate (FITC)-labeled EC-12 was cocultured with peritoneal macrophage and the amount of EC-12 phagocytosed by peritoneal macrophages was measured using a microplate fluorometer. Peritoneal macrophages from toll-like receptor (TLR)2-, TLR7-, and MyD88-deficient knockout (KO) mice exhibited similar levels of EC-12 phagocytosis to those from wild-type mice. Similarly, dectin-1 neutralization of peritoneal macrophages had no effect on EC-12 phagocytosis. However, blockade of the mannose receptor (MR) significantly decreased the amount of EC-12 phagocytosed by peritoneal macrophages; the same effect was observed in bone marrow-derived macrophages and dendritic cells. Our findings suggest that MR plays a major role in EC-12 phagocytosis by the APCs. This aim of this study was to clarify the phagocytic mechanisms of a heat-killed cell preparation of Enterococcus faecalis strain EC-12 (EC-12) by antigen-presenting cells (APCs). Our findings suggest that mannose receptor (MR) plays a major role in EC-12 phagocytosis by the APCs.

Original languageEnglish
Pages (from-to)610-617
Number of pages8
JournalMicrobiologyOpen
Volume2
Issue number4
DOIs
Publication statusPublished - Aug 1 2013

Keywords

  • Antigen-presenting cell
  • EC-12
  • LAB
  • Macrophage
  • Mannose receptor
  • Phagocytosis

ASJC Scopus subject areas

  • Microbiology

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