Abstract
The tangerine pathotype of Alternaria alternata produces host-selective ACT-toxin and causes Alternaria brown spot disease of tangerines and tangerine hybrids. Sequence analysis of a genomic BAC clone identified a previously uncharacterized portion of the ACT-toxin biosynthesis gene cluster (ACTT). A 1,034-bp gene encoding a putative enoylreductase was identified by using rapid amplification of cDNA ends and polymerase chain reaction and designated ACTTS2. Genomic Southern blots demonstrated that ACTTS2 is present only in ACT-toxin producers and is carried on a 1.9 Mb conditionally dispensable chromosome by the tangerine pathotype. Targeted gene disruption of ACTTS2 led to a reduction in ACT-toxin production and pathogenicity, and transcriptional knockdown of ACTTS2 using RNA silencing resulted in complete loss of ACT-toxin production and pathogenicity. These results indicate that ACTTS2 is an essential gene for ACT-toxin biosynthesis in the tangerine pathotype of A. alternata and is required for pathogenicity of this fungus.
| Original language | English |
|---|---|
| Pages (from-to) | 120-126 |
| Number of pages | 7 |
| Journal | Phytopathology |
| Volume | 100 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - Feb 2010 |
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Keywords
- Citrus
- Host-specific toxin
- Toxin biosynthesis gene cluster
ASJC Scopus subject areas
- Plant Science
- Agronomy and Crop Science
Cite this
Role of the host-selective ACT-toxin synthesis gene ACTTS2 encoding an enoyl-reductase in pathogenicity of the tangerine pathotype of alternaria alternata. / Ajiro, Naoya; Miyamoto, Yoko; Masunaka, Akira; Tsuge, Takashi; Yamamoto, Mikihiro; Ohtani, Kouhei; Fukumoto, Takeshi; Gomi, Kenji; Peever, Tobin L.; Izumi, Yuriko; Tada, Yasuomi; Akimitsu, Kazuya.
In: Phytopathology, Vol. 100, No. 2, 02.2010, p. 120-126.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Role of the host-selective ACT-toxin synthesis gene ACTTS2 encoding an enoyl-reductase in pathogenicity of the tangerine pathotype of alternaria alternata
AU - Ajiro, Naoya
AU - Miyamoto, Yoko
AU - Masunaka, Akira
AU - Tsuge, Takashi
AU - Yamamoto, Mikihiro
AU - Ohtani, Kouhei
AU - Fukumoto, Takeshi
AU - Gomi, Kenji
AU - Peever, Tobin L.
AU - Izumi, Yuriko
AU - Tada, Yasuomi
AU - Akimitsu, Kazuya
PY - 2010/2
Y1 - 2010/2
N2 - The tangerine pathotype of Alternaria alternata produces host-selective ACT-toxin and causes Alternaria brown spot disease of tangerines and tangerine hybrids. Sequence analysis of a genomic BAC clone identified a previously uncharacterized portion of the ACT-toxin biosynthesis gene cluster (ACTT). A 1,034-bp gene encoding a putative enoylreductase was identified by using rapid amplification of cDNA ends and polymerase chain reaction and designated ACTTS2. Genomic Southern blots demonstrated that ACTTS2 is present only in ACT-toxin producers and is carried on a 1.9 Mb conditionally dispensable chromosome by the tangerine pathotype. Targeted gene disruption of ACTTS2 led to a reduction in ACT-toxin production and pathogenicity, and transcriptional knockdown of ACTTS2 using RNA silencing resulted in complete loss of ACT-toxin production and pathogenicity. These results indicate that ACTTS2 is an essential gene for ACT-toxin biosynthesis in the tangerine pathotype of A. alternata and is required for pathogenicity of this fungus.
AB - The tangerine pathotype of Alternaria alternata produces host-selective ACT-toxin and causes Alternaria brown spot disease of tangerines and tangerine hybrids. Sequence analysis of a genomic BAC clone identified a previously uncharacterized portion of the ACT-toxin biosynthesis gene cluster (ACTT). A 1,034-bp gene encoding a putative enoylreductase was identified by using rapid amplification of cDNA ends and polymerase chain reaction and designated ACTTS2. Genomic Southern blots demonstrated that ACTTS2 is present only in ACT-toxin producers and is carried on a 1.9 Mb conditionally dispensable chromosome by the tangerine pathotype. Targeted gene disruption of ACTTS2 led to a reduction in ACT-toxin production and pathogenicity, and transcriptional knockdown of ACTTS2 using RNA silencing resulted in complete loss of ACT-toxin production and pathogenicity. These results indicate that ACTTS2 is an essential gene for ACT-toxin biosynthesis in the tangerine pathotype of A. alternata and is required for pathogenicity of this fungus.
KW - Citrus
KW - Host-specific toxin
KW - Toxin biosynthesis gene cluster
UR - http://www.scopus.com/inward/record.url?scp=75949097528&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=75949097528&partnerID=8YFLogxK
U2 - 10.1094/PHYTO-100-2-0120
DO - 10.1094/PHYTO-100-2-0120
M3 - Article
C2 - 20055645
AN - SCOPUS:75949097528
VL - 100
SP - 120
EP - 126
JO - Phytopathology
JF - Phytopathology
SN - 0031-949X
IS - 2
ER -