Role of the histone-like nucleoid structuring protein H-NS in the regulation of virulence factor expression and stress response in vibrio vulnificus

Abdelaziz Elgaml, Shin-ichi Miyoshi

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Temperature is one of the important parameters regulating the expression of virulence factors in bacteria. The global regulator, a histone-like nucleoid structuring protein H-NS, is known to play a crucial role in this regulation. In the present study, we first clarified the role of H-NS in the temperature-dependent regulation of virulence factor production in Vibrio vulnificus, including that of the cytolytic toxin V. vulnificus hemolysin: VVH and the proteolytic enzyme V. vulnificus protease: VVP. The expression of hns itself was subjected to temperature regulation, where hns was expressed more at 26°C than at 37°C. VVH production and the expression of its gene vvhA were increased by disruption of the hns gene. H-NS appeared to affect the vvhA expression by the well-documented transcriptional silencing mechanism. On the other hand, hns disruption resulted in the reduction of VVP production and the expression of its gene vvpE. H-NS was suggested to positively regulate vvpE expression through the increase in the level of the rpoS mRNA. Moreover, H-NS was found to contribute to the survival of V. vulnificus in stressful environments. When compared to the wild type strain, the hns mutant exhibited reduced survival rates when subjected to acidic pH, hyperosmotic and oxidative stress.

Original languageEnglish
Pages (from-to)263-274
Number of pages12
JournalBiocontrol Science
Volume20
Issue number4
Publication statusPublished - 2015

Fingerprint

Vibrio vulnificus
Virulence Factors
Histones
Temperature
Peptide Hydrolases
Gene Expression
Proteins
Hemolysin Proteins
Oxidative Stress
Bacteria
Messenger RNA
Genes

Keywords

  • H-ns
  • Hemolysin
  • Metalloprotease
  • Stress response
  • Temperature
  • Vibrio vulnificus

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Public Health, Environmental and Occupational Health

Cite this

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abstract = "Temperature is one of the important parameters regulating the expression of virulence factors in bacteria. The global regulator, a histone-like nucleoid structuring protein H-NS, is known to play a crucial role in this regulation. In the present study, we first clarified the role of H-NS in the temperature-dependent regulation of virulence factor production in Vibrio vulnificus, including that of the cytolytic toxin V. vulnificus hemolysin: VVH and the proteolytic enzyme V. vulnificus protease: VVP. The expression of hns itself was subjected to temperature regulation, where hns was expressed more at 26°C than at 37°C. VVH production and the expression of its gene vvhA were increased by disruption of the hns gene. H-NS appeared to affect the vvhA expression by the well-documented transcriptional silencing mechanism. On the other hand, hns disruption resulted in the reduction of VVP production and the expression of its gene vvpE. H-NS was suggested to positively regulate vvpE expression through the increase in the level of the rpoS mRNA. Moreover, H-NS was found to contribute to the survival of V. vulnificus in stressful environments. When compared to the wild type strain, the hns mutant exhibited reduced survival rates when subjected to acidic pH, hyperosmotic and oxidative stress.",
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AB - Temperature is one of the important parameters regulating the expression of virulence factors in bacteria. The global regulator, a histone-like nucleoid structuring protein H-NS, is known to play a crucial role in this regulation. In the present study, we first clarified the role of H-NS in the temperature-dependent regulation of virulence factor production in Vibrio vulnificus, including that of the cytolytic toxin V. vulnificus hemolysin: VVH and the proteolytic enzyme V. vulnificus protease: VVP. The expression of hns itself was subjected to temperature regulation, where hns was expressed more at 26°C than at 37°C. VVH production and the expression of its gene vvhA were increased by disruption of the hns gene. H-NS appeared to affect the vvhA expression by the well-documented transcriptional silencing mechanism. On the other hand, hns disruption resulted in the reduction of VVP production and the expression of its gene vvpE. H-NS was suggested to positively regulate vvpE expression through the increase in the level of the rpoS mRNA. Moreover, H-NS was found to contribute to the survival of V. vulnificus in stressful environments. When compared to the wild type strain, the hns mutant exhibited reduced survival rates when subjected to acidic pH, hyperosmotic and oxidative stress.

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