Role of the cysteine-rich region of HIV tat protein on its trans-activational ability

Satoshi Kubota; Sei Ichi Endo; Masatoshi Maki; Masakazu Hatanaka

doi:10.1007/BF00315255

Role of the cysteine-rich region of HIV tat protein on its trans-activational ability

Satoshi Kubota, Sei Ichi Endo, Masatoshi Maki, Masakazu Hatanaka

Research output: Contribution to journal › Article › peer-review

10 Citations (Scopus)

Abstract

The tat protein of human immunodeficiency virus (HIV) has a characteristic cysteine-rich region containing 7 cysteines within 16 residues. The role of this region was investigated by creation of several tat gene mutants. The activities of the novel tat gene translational products were assayed by measuring the cotransfected chloramphenicol acetyl-transferase (CAT) gene expression controlled by HIV long-terminal repeat (LTR) in the COS 7 cells. Substitution of either Cys²² with Gly, or Cys³⁴-Gln-Val-Cys with His-Gln-Val-His, and deletion behind Lys⁵⁰ of the tat protein caused a drastic loss in its activity.

Original language	English
Pages (from-to)	113-118
Number of pages	6
Journal	Virus Genes
Volume	2
Issue number	2
DOIs	https://doi.org/10.1007/BF00315255
Publication status	Published - Mar 1 1989
Externally published	Yes

Keywords

CAT assay
HIV tat protein
cysteine-rich region
site-directed mutagenesis
trans-activation
zinc-finger motif

ASJC Scopus subject areas

Molecular Biology
Genetics
Virology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1007/BF00315255

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title = "Role of the cysteine-rich region of HIV tat protein on its trans-activational ability",

abstract = "The tat protein of human immunodeficiency virus (HIV) has a characteristic cysteine-rich region containing 7 cysteines within 16 residues. The role of this region was investigated by creation of several tat gene mutants. The activities of the novel tat gene translational products were assayed by measuring the cotransfected chloramphenicol acetyl-transferase (CAT) gene expression controlled by HIV long-terminal repeat (LTR) in the COS 7 cells. Substitution of either Cys22 with Gly, or Cys34-Gln-Val-Cys with His-Gln-Val-His, and deletion behind Lys50 of the tat protein caused a drastic loss in its activity.",

keywords = "CAT assay, HIV tat protein, cysteine-rich region, site-directed mutagenesis, trans-activation, zinc-finger motif",

author = "Satoshi Kubota and Endo, {Sei Ichi} and Masatoshi Maki and Masakazu Hatanaka",

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T1 - Role of the cysteine-rich region of HIV tat protein on its trans-activational ability

AU - Kubota, Satoshi

AU - Endo, Sei Ichi

AU - Maki, Masatoshi

AU - Hatanaka, Masakazu

PY - 1989/3/1

Y1 - 1989/3/1

N2 - The tat protein of human immunodeficiency virus (HIV) has a characteristic cysteine-rich region containing 7 cysteines within 16 residues. The role of this region was investigated by creation of several tat gene mutants. The activities of the novel tat gene translational products were assayed by measuring the cotransfected chloramphenicol acetyl-transferase (CAT) gene expression controlled by HIV long-terminal repeat (LTR) in the COS 7 cells. Substitution of either Cys22 with Gly, or Cys34-Gln-Val-Cys with His-Gln-Val-His, and deletion behind Lys50 of the tat protein caused a drastic loss in its activity.

AB - The tat protein of human immunodeficiency virus (HIV) has a characteristic cysteine-rich region containing 7 cysteines within 16 residues. The role of this region was investigated by creation of several tat gene mutants. The activities of the novel tat gene translational products were assayed by measuring the cotransfected chloramphenicol acetyl-transferase (CAT) gene expression controlled by HIV long-terminal repeat (LTR) in the COS 7 cells. Substitution of either Cys22 with Gly, or Cys34-Gln-Val-Cys with His-Gln-Val-His, and deletion behind Lys50 of the tat protein caused a drastic loss in its activity.

KW - CAT assay

KW - HIV tat protein

KW - cysteine-rich region

KW - site-directed mutagenesis

KW - trans-activation

KW - zinc-finger motif

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Role of the cysteine-rich region of HIV tat protein on its trans-activational ability

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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