Role of the cysteine-rich region of HIV tat protein on its trans-activational ability

Satoshi Kubota, Sei Ichi Endo, Masatoshi Maki, Masakazu Hatanaka

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

The tat protein of human immunodeficiency virus (HIV) has a characteristic cysteine-rich region containing 7 cysteines within 16 residues. The role of this region was investigated by creation of several tat gene mutants. The activities of the novel tat gene translational products were assayed by measuring the cotransfected chloramphenicol acetyl-transferase (CAT) gene expression controlled by HIV long-terminal repeat (LTR) in the COS 7 cells. Substitution of either Cys22 with Gly, or Cys34-Gln-Val-Cys with His-Gln-Val-His, and deletion behind Lys50 of the tat protein caused a drastic loss in its activity.

Original languageEnglish
Pages (from-to)113-118
Number of pages6
JournalVirus Genes
Volume2
Issue number2
DOIs
Publication statusPublished - Mar 1989
Externally publishedYes

Fingerprint

tat Gene Products
Human Immunodeficiency Virus Proteins
Cysteine
tat Genes
HIV Long Terminal Repeat
COS Cells
Chloramphenicol
Transferases
HIV
Gene Expression

Keywords

  • CAT assay
  • cysteine-rich region
  • HIV tat protein
  • site-directed mutagenesis
  • trans-activation
  • zinc-finger motif

ASJC Scopus subject areas

  • Virology
  • Immunology
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Genetics

Cite this

Role of the cysteine-rich region of HIV tat protein on its trans-activational ability. / Kubota, Satoshi; Endo, Sei Ichi; Maki, Masatoshi; Hatanaka, Masakazu.

In: Virus Genes, Vol. 2, No. 2, 03.1989, p. 113-118.

Research output: Contribution to journalArticle

Kubota, Satoshi ; Endo, Sei Ichi ; Maki, Masatoshi ; Hatanaka, Masakazu. / Role of the cysteine-rich region of HIV tat protein on its trans-activational ability. In: Virus Genes. 1989 ; Vol. 2, No. 2. pp. 113-118.
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