Role of the cysteine-rich region of HIV tat protein on its trans-activational ability

Satoshi Kubota, Sei Ichi Endo, Masatoshi Maki, Masakazu Hatanaka

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

The tat protein of human immunodeficiency virus (HIV) has a characteristic cysteine-rich region containing 7 cysteines within 16 residues. The role of this region was investigated by creation of several tat gene mutants. The activities of the novel tat gene translational products were assayed by measuring the cotransfected chloramphenicol acetyl-transferase (CAT) gene expression controlled by HIV long-terminal repeat (LTR) in the COS 7 cells. Substitution of either Cys22 with Gly, or Cys34-Gln-Val-Cys with His-Gln-Val-His, and deletion behind Lys50 of the tat protein caused a drastic loss in its activity.

Original languageEnglish
Pages (from-to)113-118
Number of pages6
JournalVirus Genes
Volume2
Issue number2
DOIs
Publication statusPublished - Mar 1 1989

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Keywords

  • CAT assay
  • HIV tat protein
  • cysteine-rich region
  • site-directed mutagenesis
  • trans-activation
  • zinc-finger motif

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Virology

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