Role of superoxide generation and degradation system of mast cells in allergic inflammation

M. Akagi, N. Fukuishi, Masakiyo Sakaguchi, N. Matsui, H. Takahashi

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Rat peritoneal mast cells are stimulated to generate superoxide anion (O2) by the addition of compound 48/80 and A23187. Recently, we demonstrated by immunohistochemical and Western blot analysis that the mast cells contained the p47phox protein, which was one of cytosolic component of the NADPH oxidase system. In the present study, it was demonstrated that the mast cells contained the p47phox mRNA, much similar to that of mouse leukocyte. The permeabilized mast cells were stimulated to generate O2- by the addition of Ca2+, phospholipase A2 (PLA2) and arachidonic acid. Our data suggest the following:(1) cytosolic PLA2 may be activated by the elevation of [Ca2+]i; (2) the conjugation of membrane component with cytosolic component may be stimulated by the released arachidonic acid. The mast cell granules contained superoxide dismutase (SOD)-like enzyme, which degradated O2-, generated in xanthine-xanthinoxidase system. SOD-like enzyme was released from the granules by the treatment with Ca2+ and trapped by the treatment with heparin. In conclusion, our studies suggest that the disorder of the degradation system of O2- may contribute to the development of allergic inflammation.

Original languageEnglish
JournalFolia Pharmacologica Japonica
Volume112 Suppl 1
Publication statusPublished - Oct 1998
Externally publishedYes

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Mast Cells
Superoxides
Inflammation
Arachidonic Acid
Superoxide Dismutase
Cytosolic Phospholipases A2
p-Methoxy-N-methylphenethylamine
Xanthine
NADPH Oxidase
Phospholipases A2
Calcimycin
Enzymes
Heparin
Leukocytes
Western Blotting
Messenger RNA
Membranes
Proteins

ASJC Scopus subject areas

  • Pharmacology

Cite this

Role of superoxide generation and degradation system of mast cells in allergic inflammation. / Akagi, M.; Fukuishi, N.; Sakaguchi, Masakiyo; Matsui, N.; Takahashi, H.

In: Folia Pharmacologica Japonica, Vol. 112 Suppl 1, 10.1998.

Research output: Contribution to journalArticle

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