Role of sarcolemmal BK Ca channels in stretch-induced extrasystoles in isolated chick hearts

Gentaro Iribe, Honghua Jin, Keiji Naruse

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Background: It remains unclear whether sarcolemmal BK Ca channels in post-hatch chick ventricular myocytes contribute to stretch-induced extrasystoles (SIE), and whether they are stretch-activated BK Ca (SAK Ca) channels or a non-stretch-sensitive BK Ca variant. Methods and Results: To determine the role of sarcolemmal BK Ca channels in SIE and their stretch sensitivity, an isolated 2-week-old Langendorff-perfused chick heart and mathematical simulation were used. The ventricular wall was rapidly stretched by application of a volume change pulse. As the speed of the stretch increased, the probability of SIE also significantly increased, significantly shortening the delay between SIE and the initiation of the stretch. Application of 100 nmol/L of Grammostola spatulata mechanotoxin 4, a cation-selective stretch-activated channel (SAC) blocker, significantly decreased the probability of SIE. The application of Iberiotoxin, however, a BK Ca channel blocker, significantly increased the probability of SIE, suggesting that a K + efflux via a sarcolemmal BK Ca channel reduces SIE by balancing out stretch-induced cation influx via SACs. The simulation using a cardiomyocyte model combined with a new stretch sensitivity model that considers viscoelastic intracellular force transmission showed that stretch sensitivity in BK Ca channels is required to reproduce the present wet experimental results. Conclusions: Sarcolemmal BK Ca channels in post-hatch chick ventricular myocytes are SAK Ca channels, and they have a suppressive effect on SIE.

Original languageEnglish
Pages (from-to)2552-2558
Number of pages7
JournalCirculation Journal
Volume75
Issue number11
DOIs
Publication statusPublished - 2011

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Large-Conductance Calcium-Activated Potassium Channels
Premature Cardiac Complexes
Muscle Cells
Cations
Cardiac Myocytes
Pulse

Keywords

  • Arrhythmia
  • Modeling
  • Stretch-activated channels

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

Cite this

Role of sarcolemmal BK Ca channels in stretch-induced extrasystoles in isolated chick hearts. / Iribe, Gentaro; Jin, Honghua; Naruse, Keiji.

In: Circulation Journal, Vol. 75, No. 11, 2011, p. 2552-2558.

Research output: Contribution to journalArticle

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AB - Background: It remains unclear whether sarcolemmal BK Ca channels in post-hatch chick ventricular myocytes contribute to stretch-induced extrasystoles (SIE), and whether they are stretch-activated BK Ca (SAK Ca) channels or a non-stretch-sensitive BK Ca variant. Methods and Results: To determine the role of sarcolemmal BK Ca channels in SIE and their stretch sensitivity, an isolated 2-week-old Langendorff-perfused chick heart and mathematical simulation were used. The ventricular wall was rapidly stretched by application of a volume change pulse. As the speed of the stretch increased, the probability of SIE also significantly increased, significantly shortening the delay between SIE and the initiation of the stretch. Application of 100 nmol/L of Grammostola spatulata mechanotoxin 4, a cation-selective stretch-activated channel (SAC) blocker, significantly decreased the probability of SIE. The application of Iberiotoxin, however, a BK Ca channel blocker, significantly increased the probability of SIE, suggesting that a K + efflux via a sarcolemmal BK Ca channel reduces SIE by balancing out stretch-induced cation influx via SACs. The simulation using a cardiomyocyte model combined with a new stretch sensitivity model that considers viscoelastic intracellular force transmission showed that stretch sensitivity in BK Ca channels is required to reproduce the present wet experimental results. Conclusions: Sarcolemmal BK Ca channels in post-hatch chick ventricular myocytes are SAK Ca channels, and they have a suppressive effect on SIE.

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