Role of recA/RAD51 family proteins in mammals

Masahiro Kawabata, Teruyuki Kawabata, Masahiro Nishibori

Research output: Contribution to journalArticle

64 Citations (Scopus)

Abstract

DNA damage causes chromosomal instability leading to oncogenesis, apoptosis, and severe failure of cell functions. The DNA repair system includes base excision repair, nucleotide excision repair, mismatch repair, translesion replication, non-homologous end-joining, and recombinational repair. Homologous recombination performs the recombinational repair. The RAD51 gene is an ortholog of Esherichia coli recA, and the gene product Rad51 protein plays a central role in the homologous recombination. In mammals, 7 recA-like genes have been identified: RAD51, RAD51L1/B, RAD51L2/ C, RAD51L3/ D, XRCC2, XRCC3, and DMC1. These genes, with the exception of meiosis-specific DMC1, are essential for development in mammals. Disruption of the RAD51 gene leads to cell death, whereas RAD51L1/ B, RAD51L2/ C, RAD51L3/ D, XRCC2, and XRCC3 genes (RAD51 paralogs) are not essential for viability of cells, but these gene-deficient cells exhibit a similar defective phenotype. Yeast two-hybrid analysis, co-immunoprecipitation, mutation analysis, and domain mapping of Rad51 and Rad51 paralogs have revealed protein-protein interactions among these gene products. Recent investigations have shown that Rad51 paralogs play a role not only in an early step, but also in a late step of homologous recombination. In addition, identification of alternative transcripts of some RAD51 paralogs may reflect the complexity of the homologous recombination system. Copyright

Original languageEnglish
Pages (from-to)1-9
Number of pages9
JournalActa Medica Okayama
Volume59
Issue number1
Publication statusPublished - Feb 2005

Fingerprint

Rad51 Recombinase
Mammals
Genes
Homologous Recombination
Repair
DNA Repair
Proteins
Chromosomal Instability
DNA Mismatch Repair
Meiosis
DNA
Immunoprecipitation
Cell death
DNA Damage
Joining
Cell Survival
Yeast
Carcinogenesis
Cell Death
Yeasts

Keywords

  • DNA repair
  • RAD51
  • RAD51 paralogs
  • RecA
  • Recombination

ASJC Scopus subject areas

  • Medicine(all)
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Role of recA/RAD51 family proteins in mammals. / Kawabata, Masahiro; Kawabata, Teruyuki; Nishibori, Masahiro.

In: Acta Medica Okayama, Vol. 59, No. 1, 02.2005, p. 1-9.

Research output: Contribution to journalArticle

Kawabata, M, Kawabata, T & Nishibori, M 2005, 'Role of recA/RAD51 family proteins in mammals', Acta Medica Okayama, vol. 59, no. 1, pp. 1-9.
Kawabata, Masahiro ; Kawabata, Teruyuki ; Nishibori, Masahiro. / Role of recA/RAD51 family proteins in mammals. In: Acta Medica Okayama. 2005 ; Vol. 59, No. 1. pp. 1-9.
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