Role of prostaglandin D2 and E2 terminal synthases in chronic rhinosinusitis

M. Okano, T. Fujiwara, M. Yamamoto, Y. Sugata, R. Matsumoto, K. Fukushima, Tadashi Yoshino, K. Shimizu, N. Eguchi, M. Kiniwa, Y. Urade, Kazunori Nishizaki

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

Background Prostaglandin (PG)D2 and E2, two major cyclooxygenase (COX) products, are generated by PGD2 synthase (PGDS) and PGE2 synthase (PGES), respectively, and appear to mediate airway inflammation. Objective We sought to determine the role of PGDS and PGES in the pathophysiology of chronic rhinosinusitis (CRS). Methods The study examined the expression of PGDS and PGES in nasal polyps of 22 CRS patients. As controls, uncinate process mucosae were obtained from 12 CRS patients not having nasal polyps and five subjects without sinusitis. Immunohistochemistry and quantitative real-time PCR were used to evaluate the expression. Results Both PGDS and PGES were detected in nasal polyps by immunohistochemistry. Significantly greater levels of PGDS mRNA and lesser levels of PGES mRNA were observed in the nasal polyps as compared with uncinate process mucosae, and an inverse correlation between PGDS and PGES expression was observed. Levels of PGDS mRNA in nasal polyps were positively correlated with degree of infiltration by EG2+ eosinophils, whereas the levels of PGES were inversely correlated. Significantly increased levels of PGDS and conversely decreased levels of PGES were observed in asthmatics as compared with non-asthmatics. In addition, PGDS and PGES levels were positively and inversely correlated with the radiological severity of sinusitis, respectively. Conclusions These results suggest that PGDS and PGES display an opposite and important role in the pathophysiology of CRS such as polyp formation, and more specifically, a biased expression of these synthases might contribute to the development of CRS by affecting eosinophilic inflammation.

Original languageEnglish
Pages (from-to)1028-1038
Number of pages11
JournalClinical and Experimental Allergy
Volume36
Issue number8
DOIs
Publication statusPublished - Aug 2006

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prostaglandin R2 D-isomerase
Prostaglandin D2
Dinoprostone
Nasal Polyps
Sinusitis
Messenger RNA
Mucous Membrane
Immunohistochemistry
Prostaglandin-E Synthases
Inflammation

Keywords

  • Eosinophil
  • PGD
  • PGE
  • Sinusitis
  • Synthase

ASJC Scopus subject areas

  • Immunology

Cite this

Role of prostaglandin D2 and E2 terminal synthases in chronic rhinosinusitis. / Okano, M.; Fujiwara, T.; Yamamoto, M.; Sugata, Y.; Matsumoto, R.; Fukushima, K.; Yoshino, Tadashi; Shimizu, K.; Eguchi, N.; Kiniwa, M.; Urade, Y.; Nishizaki, Kazunori.

In: Clinical and Experimental Allergy, Vol. 36, No. 8, 08.2006, p. 1028-1038.

Research output: Contribution to journalArticle

Okano, M, Fujiwara, T, Yamamoto, M, Sugata, Y, Matsumoto, R, Fukushima, K, Yoshino, T, Shimizu, K, Eguchi, N, Kiniwa, M, Urade, Y & Nishizaki, K 2006, 'Role of prostaglandin D2 and E2 terminal synthases in chronic rhinosinusitis', Clinical and Experimental Allergy, vol. 36, no. 8, pp. 1028-1038. https://doi.org/10.1111/j.1365-2222.2006.02528.x
Okano M, Fujiwara T, Yamamoto M, Sugata Y, Matsumoto R, Fukushima K et al. Role of prostaglandin D2 and E2 terminal synthases in chronic rhinosinusitis. Clinical and Experimental Allergy. 2006 Aug;36(8):1028-1038. https://doi.org/10.1111/j.1365-2222.2006.02528.x
Okano, M. ; Fujiwara, T. ; Yamamoto, M. ; Sugata, Y. ; Matsumoto, R. ; Fukushima, K. ; Yoshino, Tadashi ; Shimizu, K. ; Eguchi, N. ; Kiniwa, M. ; Urade, Y. ; Nishizaki, Kazunori. / Role of prostaglandin D2 and E2 terminal synthases in chronic rhinosinusitis. In: Clinical and Experimental Allergy. 2006 ; Vol. 36, No. 8. pp. 1028-1038.
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AB - Background Prostaglandin (PG)D2 and E2, two major cyclooxygenase (COX) products, are generated by PGD2 synthase (PGDS) and PGE2 synthase (PGES), respectively, and appear to mediate airway inflammation. Objective We sought to determine the role of PGDS and PGES in the pathophysiology of chronic rhinosinusitis (CRS). Methods The study examined the expression of PGDS and PGES in nasal polyps of 22 CRS patients. As controls, uncinate process mucosae were obtained from 12 CRS patients not having nasal polyps and five subjects without sinusitis. Immunohistochemistry and quantitative real-time PCR were used to evaluate the expression. Results Both PGDS and PGES were detected in nasal polyps by immunohistochemistry. Significantly greater levels of PGDS mRNA and lesser levels of PGES mRNA were observed in the nasal polyps as compared with uncinate process mucosae, and an inverse correlation between PGDS and PGES expression was observed. Levels of PGDS mRNA in nasal polyps were positively correlated with degree of infiltration by EG2+ eosinophils, whereas the levels of PGES were inversely correlated. Significantly increased levels of PGDS and conversely decreased levels of PGES were observed in asthmatics as compared with non-asthmatics. In addition, PGDS and PGES levels were positively and inversely correlated with the radiological severity of sinusitis, respectively. Conclusions These results suggest that PGDS and PGES display an opposite and important role in the pathophysiology of CRS such as polyp formation, and more specifically, a biased expression of these synthases might contribute to the development of CRS by affecting eosinophilic inflammation.

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KW - Synthase

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