Role of P-glycoprotein in all-trans retinoic acid (ATRA) resistance in acute promyelocytic leukaemia cells: Analysis of intracellular concentration of ATRA

Akihiro Takeshita, Kaori Shinjo, Kensuke Naito, Kazunori Ohnishi, Yoshikazu Sugimoto, Yoshinori Yamakawa, Mitsune Tanimoto, Kunio Kitamura, Tumoki Naoe, Ryuzo Ohno

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

We analysed the relationship between all-trans retinoic acid (ATRA) resistance and P-glycoprotein (P-gp)-associated multidrug resistance (MDR) in acute promyelocytic leukaemia (APL). There was no difference in the intracellular ATRA accumulation between NB4 cells and an MDR1 cDNA-transduced NB4 subline and between ATRA-resistant NB4 cells (NB4/RA) and an MDR1 cDNA- transduced NB4/RA subline. PSC833, a MDR modifier, did not increase the intracellular accumulation of ATRA or affect the expression of CD11b, the nitroblue tetrazolium (NBT) reduction activity, the proportion of apoptotic cells or the morphology of these four ATRA-treated cell lines. Similar results were obtained in the analysis of APL cells from five patients relapsed after ATRA-induced complete remission.

Original languageEnglish
Pages (from-to)90-92
Number of pages3
JournalBritish Journal of Haematology
Volume108
Issue number1
DOIs
Publication statusPublished - 2000

Keywords

  • Acute promyelocytic leukaemia
  • All-trans retinoic acid
  • Multidrug resistance
  • P-glycoprotein

ASJC Scopus subject areas

  • Hematology

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