Role of NMDA receptor upon [14C]acetate uptake into intact rat brain

Shinichiro Hirose, Sotaro Momosaki, Rie Hosoi, Kohji Abe, Antony Gee, Osamu Inoue

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Objective: To clarify the role of N-methyl-d-aspartate (NMDA) receptors upon [14C]acetate uptake in the rodent central nervous system (CNS), ibotenic acid (IBO) was infused into the right striatum of the rat brain. Methods: Autoradiograms of [14C]acetate uptake in the brain for 2 h following the infusion of IBO (10 μg/μl) were obtained in both non-treated and MK-801 (1 mg/kg, i.v.) pretreated rats. The effect of MK-801 on [ 14C]acetate uptake in the normal rat brain was also studied. Results: Infusion of IBO significantly decreased [14C]acetate uptake in the infused side of the striatum. The expression of monocarboxylate transporter-1 was not altered, suggesting that the activity of tricarboxylic acid (TCA) cycle in glial cells might be depressed. Pretreatment with MK-801 completely blocked the decreasing effect of IBO on [14C]acetate uptake. MK-801 also increased [14C]acetate uptake in the whole brain of normal rats. Conclusions: These results indicate the important roles of NMDA receptors on [14C]acetate uptake in the intact rat brain.

Original languageEnglish
Pages (from-to)143-147
Number of pages5
JournalAnnals of nuclear medicine
Volume23
Issue number2
DOIs
Publication statusPublished - Feb 1 2009
Externally publishedYes

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Keywords

  • Glia
  • Ibotenic acid
  • MK-801
  • Rat
  • [C]acetate

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging

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