Role of nitric oxide in the regulation of superoxide dismutase and prostaglandin F production in bovine luteal endothelial cells

Seunghyung Lee, Tomas J. Acosta, Yuji Nakagawa, Kiyoshi Okuda

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Prostaglandin F (PGF) induces a rapid reduction in progesterone production (functional luteolysis) followed by tissue degeneration and cell death (structural luteolysis). Reactive oxygen species (ROS) including nitric oxide (NO) play crucial roles in the luteolytic action of PGF. The local concentration of intraluteal ROS is controlled by superoxide dismutase (SOD), the main enzyme involved in the control of intraluteal ROS. To clarify the roles of NO in the regulation of SOD in luteolysis, we examined the effects of NO on SOD expression and activity in cultured bovine luteal endothelial cells (LECs) during short-term (2 h, mimicking functional luteolysis) and long-term (24 h, mimicking structural luteolysis) incubation. We also investigated whether NO modulates PGF production by LECs. LECs were isolated from mid-luteal phase CLs, and exposed to NONOate (a NO donor) for 2 or 24 h. SOD mRNA expression was stimulated by NONOate (10-100 μM) at 2 h (P<0.05). Moreover, 10 μM NONOate stimulated SOD protein expression and SOD activity at 2 h (P<0.05), whereas NONOate inhibited SOD mRNA and protein expressions at 24 h (P<0.05). NONOate stimulated PGF biosynthesis at both incubation times. The overall findings suggest that NO differently regulates SOD in cultured LECs, depending on the exposure time. Acute elevation of SOD may represent a response of LECs to protect themselves against oxidative stress induced by PGF during functional luteolysis, whereas a later reduction of SOD levels by NO may facilitate an excess of intraluteal ROS during structural luteolysis.

Original languageEnglish
Pages (from-to)454-459
Number of pages6
JournalJournal of Reproduction and Development
Volume56
Issue number4
DOIs
Publication statusPublished - Aug 1 2010

Keywords

  • Luteal endothelial cell
  • Nitric oxide
  • Superoxide dismutase

ASJC Scopus subject areas

  • Animal Science and Zoology

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