Role of NF-κB on liver cold ischemia-reperfusion injury

Yoshihito Takahashi, Raymond W. Ganster, Andrea Gambotto, Lifang Shao, Takashi Kaizu, Tong Wu, Gautam P. Yagnik, Atsunori Nakao, George Tsoulfas, Takashi Ishikawa, Toyokazu Okuda, David A. Geller, Noriko Murase

Research output: Contribution to journalArticle

70 Citations (Scopus)

Abstract

The role of NF-κB, the rapid-response transcription factor for multiple genes, in cold ischemia-reperfusion (I/R) injury was examined after syngeneic transplantation of liver grafts. Lewis rat recipients were killed 1-48 h after reperfusion of three different liver grafts: 1) uninfected control, 2) infected ex vivo with control adenoviral vector (AdEGFP), and 3) infected ex vivo with AdIκB. In uninfected control livers, NF-κB was activated biphasically at 1-3 and 12 h after reperfusion with aspartate transaminase (AST) levels of 4,244 ± 691 IU/l. The first peak of NF-κB activation associated with an increase of mRNA for TNF-α, IL-1β, and IL-10. AdEGFP transfection resulted in similar outcomes. Interestingly, AdIκB-transfected liver grafts suffered more severe I/R injury (AST

Original languageEnglish
JournalAmerican Journal of Physiology - Gastrointestinal and Liver Physiology
Volume283
Issue number5 46-5
Publication statusPublished - Nov 2002
Externally publishedYes

Fingerprint

Cold Ischemia
Reperfusion Injury
Liver
Aspartate Aminotransferases
Transplants
Reperfusion
Isogeneic Transplantation
Interleukin-1
Interleukin-10
Transfection
Transcription Factors
Messenger RNA
Genes

Keywords

  • Adenoviral vector
  • IκB
  • Liver transplantation
  • Preservation injuries

ASJC Scopus subject areas

  • Gastroenterology
  • Physiology

Cite this

Takahashi, Y., Ganster, R. W., Gambotto, A., Shao, L., Kaizu, T., Wu, T., ... Murase, N. (2002). Role of NF-κB on liver cold ischemia-reperfusion injury. American Journal of Physiology - Gastrointestinal and Liver Physiology, 283(5 46-5).

Role of NF-κB on liver cold ischemia-reperfusion injury. / Takahashi, Yoshihito; Ganster, Raymond W.; Gambotto, Andrea; Shao, Lifang; Kaizu, Takashi; Wu, Tong; Yagnik, Gautam P.; Nakao, Atsunori; Tsoulfas, George; Ishikawa, Takashi; Okuda, Toyokazu; Geller, David A.; Murase, Noriko.

In: American Journal of Physiology - Gastrointestinal and Liver Physiology, Vol. 283, No. 5 46-5, 11.2002.

Research output: Contribution to journalArticle

Takahashi, Y, Ganster, RW, Gambotto, A, Shao, L, Kaizu, T, Wu, T, Yagnik, GP, Nakao, A, Tsoulfas, G, Ishikawa, T, Okuda, T, Geller, DA & Murase, N 2002, 'Role of NF-κB on liver cold ischemia-reperfusion injury', American Journal of Physiology - Gastrointestinal and Liver Physiology, vol. 283, no. 5 46-5.
Takahashi Y, Ganster RW, Gambotto A, Shao L, Kaizu T, Wu T et al. Role of NF-κB on liver cold ischemia-reperfusion injury. American Journal of Physiology - Gastrointestinal and Liver Physiology. 2002 Nov;283(5 46-5).
Takahashi, Yoshihito ; Ganster, Raymond W. ; Gambotto, Andrea ; Shao, Lifang ; Kaizu, Takashi ; Wu, Tong ; Yagnik, Gautam P. ; Nakao, Atsunori ; Tsoulfas, George ; Ishikawa, Takashi ; Okuda, Toyokazu ; Geller, David A. ; Murase, Noriko. / Role of NF-κB on liver cold ischemia-reperfusion injury. In: American Journal of Physiology - Gastrointestinal and Liver Physiology. 2002 ; Vol. 283, No. 5 46-5.
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