Role of lentiviral lytic polypeptide I (LLP-I) in the selective cytotoxicity of γ-glutamylcysteine ethyl ester against human immunodeficiency virus type 1-producing cells

Satoshi Kubota, Hui Zhang, Shigehisa Kitahara, Roger J. Pomerantz

Research output: Contribution to journalArticle

Abstract

Selective cytotoxic effects of γ-glutamylcysteine ethyl ester (γ-GCE) against human immunodeficiency virus type 1 (HIV-1)-infected H-9 T lymphocytic cells were demonstrated previously. However, the mechanism of those effects remained unclear. Here, we report on enhanced cytotoxicity of the lentiviral lytic peptide I (LLP-I) of gp41, the envelope transmembrane glycoprotein of HIV-1, in the presence of γ-GCE. Without γ-GCE, the cytotoxic effect of LLP-I was transient, whereas with γ-GCE, cell death induced by LLP-I remained continuous until termination. Of note, such effects by γ-GCE were also observed with another unrelated amphipathic peptide toxin, melittin. These results suggest that the synergistic cytotoxic effect of γ-GCE and LLP-I may play a central role in the molecular mechanism of the selective cytotoxicity of γ-GCE in HIV-1-infected T lymphocytic cells.

Original languageEnglish
Pages (from-to)121-127
Number of pages7
JournalAntiviral Chemistry and Chemotherapy
Volume10
Issue number3
Publication statusPublished - May 1999
Externally publishedYes

Keywords

  • Antioxidant
  • HIV-1
  • LLP-I

ASJC Scopus subject areas

  • Pharmacology
  • Drug Discovery
  • Virology

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