TY - JOUR
T1 - Role of intraluteal prostaglandin F2α, progesterone and oxytocin in basal and pulsatile progesterone release from developing bovine corpus luteum
AU - Bah, Mamadou M.
AU - Acosta, Tomas J.
AU - Pilawski, Wojciech
AU - Deptula, Katarzyna
AU - Okuda, Kiyoshi
AU - Skarzynski, Dariusz J.
N1 - Funding Information:
This research was supported by a Grants in Aid for Scientific Research from Polish Ministry of Sciences and Informations (PBZ-KBN-084/P06/2002/Zad.Bad. 5-2) and from Japan Society for the Promotion of Science (No. 17580246). TJA was supported by the Japanese-Polish Joint Exchange Program under the agreement between Japanese Society for Promotion of Sciences and the Polish Academy of Sciences. We thank Dr. Seiji Ito of Kansai Medical University, Osaka, Japan for antisera of prostaglandin F 2α , Dr. Stanislaw Okrasa of University of Warmia and Mazury for progesterone antisera, Dr. Per Melin of Ferring AB, Malmö, Sweden for the oxytocin antagonist and Dr. Krzysztof Chwalisz of Schering AG, Berlin, Germany for the progesterone antagonist. The authors also thank the National Hormone and Pituitary Program, University of Maryland School of Medicine and the National Institute of Diabetes and Digestive and Kidney Disease for bovine LH (USDA-bLH-B6).
PY - 2006/5
Y1 - 2006/5
N2 - The present study examined the role of intra-luteal prostaglandin (PG) F2α, progesterone (P4) and oxytocin (OT) on the corpus luteum function by using specific hormone antagonists. Luteal cells from the developing CL (days 5-7 of the estrous cycle) were exposed to P4 antagonist (onapristone, OP, 10-4 M), OT antagonist (atosiban, AT; 10-6 M) or indomethacin (INDO; 10-4 M), for 12 h and then stimulated with PGF2α (10-8 M) for 4 h. Pre-treatment of the cells with OP, AT or INDO resulted in an increase in P4 secretion in response to PGF2α. To examine the temporal effects of P4, OT and PGs on P4 secretion, dispersed luteal cells were pre-exposed to OP, AT or INDO for 1, 2, 4, 6 or 12 h. Prostaglandin F2α stimulated P4 secretion (P < 0.05) after 2 h of pre-exposition. In the microdyalisis study, the spontaneous release of P4 from developing CL tissue was of pulsatile nature with irregular peaks at 1-2 h intervals. Treatment with OP increased the number of P4 peaks (P < 0.05), whereas AT and INDO significantly reduced the number of P4 peaks detected (P < 0.05). Interestingly, INDO completely blocked the pulsatile nature in the release of P4, but it secretion remained stable troughout the experimental period. These results demonstrate that luteal PGF2α, OT, and P4 are components of an autocrine/paracrine intra-ovarian regulatory system responsible for the episodic (pulsatile) release of P4 from the bovine CL during the early luteal phase.
AB - The present study examined the role of intra-luteal prostaglandin (PG) F2α, progesterone (P4) and oxytocin (OT) on the corpus luteum function by using specific hormone antagonists. Luteal cells from the developing CL (days 5-7 of the estrous cycle) were exposed to P4 antagonist (onapristone, OP, 10-4 M), OT antagonist (atosiban, AT; 10-6 M) or indomethacin (INDO; 10-4 M), for 12 h and then stimulated with PGF2α (10-8 M) for 4 h. Pre-treatment of the cells with OP, AT or INDO resulted in an increase in P4 secretion in response to PGF2α. To examine the temporal effects of P4, OT and PGs on P4 secretion, dispersed luteal cells were pre-exposed to OP, AT or INDO for 1, 2, 4, 6 or 12 h. Prostaglandin F2α stimulated P4 secretion (P < 0.05) after 2 h of pre-exposition. In the microdyalisis study, the spontaneous release of P4 from developing CL tissue was of pulsatile nature with irregular peaks at 1-2 h intervals. Treatment with OP increased the number of P4 peaks (P < 0.05), whereas AT and INDO significantly reduced the number of P4 peaks detected (P < 0.05). Interestingly, INDO completely blocked the pulsatile nature in the release of P4, but it secretion remained stable troughout the experimental period. These results demonstrate that luteal PGF2α, OT, and P4 are components of an autocrine/paracrine intra-ovarian regulatory system responsible for the episodic (pulsatile) release of P4 from the bovine CL during the early luteal phase.
KW - Cattle
KW - Corpus luteum
KW - Oxytocin
KW - PGF
KW - Progesterone
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UR - http://www.scopus.com/inward/citedby.url?scp=33646076975&partnerID=8YFLogxK
U2 - 10.1016/j.prostaglandins.2006.01.002
DO - 10.1016/j.prostaglandins.2006.01.002
M3 - Article
C2 - 16647636
AN - SCOPUS:33646076975
VL - 79
SP - 218
EP - 229
JO - Journal of Lipid Mediators
JF - Journal of Lipid Mediators
SN - 1098-8823
IS - 3-4
ER -