Role of intraluteal prostaglandin F, progesterone and oxytocin in basal and pulsatile progesterone release from developing bovine corpus luteum

Mamadou M. Bah, Tomas J. Acosta, Wojciech Pilawski, Katarzyna Deptula, Kiyoshi Okuda, Dariusz J. Skarzynski

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

The present study examined the role of intra-luteal prostaglandin (PG) F, progesterone (P4) and oxytocin (OT) on the corpus luteum function by using specific hormone antagonists. Luteal cells from the developing CL (days 5-7 of the estrous cycle) were exposed to P4 antagonist (onapristone, OP, 10-4 M), OT antagonist (atosiban, AT; 10-6 M) or indomethacin (INDO; 10-4 M), for 12 h and then stimulated with PGF (10-8 M) for 4 h. Pre-treatment of the cells with OP, AT or INDO resulted in an increase in P4 secretion in response to PGF. To examine the temporal effects of P4, OT and PGs on P4 secretion, dispersed luteal cells were pre-exposed to OP, AT or INDO for 1, 2, 4, 6 or 12 h. Prostaglandin F stimulated P4 secretion (P < 0.05) after 2 h of pre-exposition. In the microdyalisis study, the spontaneous release of P4 from developing CL tissue was of pulsatile nature with irregular peaks at 1-2 h intervals. Treatment with OP increased the number of P4 peaks (P < 0.05), whereas AT and INDO significantly reduced the number of P4 peaks detected (P < 0.05). Interestingly, INDO completely blocked the pulsatile nature in the release of P4, but it secretion remained stable troughout the experimental period. These results demonstrate that luteal PGF, OT, and P4 are components of an autocrine/paracrine intra-ovarian regulatory system responsible for the episodic (pulsatile) release of P4 from the bovine CL during the early luteal phase.

Original languageEnglish
Pages (from-to)218-229
Number of pages12
JournalProstaglandins and Other Lipid Mediators
Volume79
Issue number3-4
DOIs
Publication statusPublished - May 1 2006

Keywords

  • Cattle
  • Corpus luteum
  • Oxytocin
  • PGF
  • Progesterone

ASJC Scopus subject areas

  • Biochemistry
  • Physiology
  • Pharmacology
  • Cell Biology

Fingerprint Dive into the research topics of 'Role of intraluteal prostaglandin F<sub>2α</sub>, progesterone and oxytocin in basal and pulsatile progesterone release from developing bovine corpus luteum'. Together they form a unique fingerprint.

  • Cite this