Role of interleukin-1 in endotoxin-induced lung injury in the rat.

C. E. Rose, C. A. Juliano, D. E. Tracey, Teizo Yoshimura, S. M. Fu

Research output: Contribution to journalArticle

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Abstract

The effects of the recombinant interleukin-1 receptor antagonist (rIL-1ra) on the systemic vascular and lung injury following intraperitoneal Salmonella enteritidis lipopolysaccharide (LPS) were determined in male Sprague-Dawley rats. Initial experiments identified that maximal mortality occurred with an intraperitoneal LPS dose of 20 mg/kg, and this dose was used in subsequent experiments. Albumin permeability, measured in an ex vivo perfused heart-lung preparation from the rats 2 h after injection of LPS, was increased with endotoxin as was the wet:dry weight ratio. Pretreatment of the rats with intravenous rIL-1ra, 1 to 10 mg/kg, followed by a continuous intravenous infusion at 30 to 50 micrograms/kg/min resulted in restoration of blood pressure at 100 min following endotoxin administration. Moreover, coadministration of rIL-1ra with endotoxin totally prevented the rise in albumin permeability of the pulmonary vasculature and the increase in wet:dry lung weight ratios observed in rats treated with LPS alone. LPS injected intraperitoneally caused a marked decrease in circulating leukocyte count, an effect not reversed by rIL-1ra. RNA extraction of whole-lung homogenates revealed that mRNA for IL-1 beta was constitutively expressed in the absence of endotoxin, but transcripts increased progressively from 0.5 to 2 h after endotoxin administration. Increases in mRNAs for tumor necrosis factor-alpha (TNF-alpha) and for macrophage inflammatory protein-2 (MIP-2), a potent neutrophil chemoattractant, were also observed from 0.5 until 2 h after endotoxin administration.(ABSTRACT TRUNCATED AT 250 WORDS)

Original languageEnglish
Pages (from-to)214-221
Number of pages8
JournalAmerican Journal of Respiratory Cell and Molecular Biology
Volume10
Issue number2
Publication statusPublished - Feb 1994
Externally publishedYes

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Lung Injury
Interleukin-1
Endotoxins
Rats
Interleukin-1 Receptors
Lipopolysaccharides
Lung
Albumins
Permeability
Chemokine CXCL2
Weights and Measures
Salmonella enteritidis
Messenger RNA
Salmonella
Vascular System Injuries
Chemotactic Factors
Blood pressure
Pulmonary Edema
Interleukin-1beta
Leukocyte Count

ASJC Scopus subject areas

  • Medicine(all)
  • Molecular Biology
  • Pulmonary and Respiratory Medicine
  • Clinical Biochemistry
  • Cell Biology

Cite this

Role of interleukin-1 in endotoxin-induced lung injury in the rat. / Rose, C. E.; Juliano, C. A.; Tracey, D. E.; Yoshimura, Teizo; Fu, S. M.

In: American Journal of Respiratory Cell and Molecular Biology, Vol. 10, No. 2, 02.1994, p. 214-221.

Research output: Contribution to journalArticle

Rose, C. E. ; Juliano, C. A. ; Tracey, D. E. ; Yoshimura, Teizo ; Fu, S. M. / Role of interleukin-1 in endotoxin-induced lung injury in the rat. In: American Journal of Respiratory Cell and Molecular Biology. 1994 ; Vol. 10, No. 2. pp. 214-221.
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