Role of helper T cells in the humoral immune responses against 53-kDa outer membrane protein from Porphymmonas gingivalis

N. Kato, Hideki Ohyama, F. Nishimura, S. Matsushita, Shogo Takashiba, Y. Murayama

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Outer membrane protein with a 53-kDa molecular weight (Ag53) isolated from Porphyromonas gingivalis evokes strong humoral immune responses in many periodontitis patients. To examine the effects of cytokines produced by Ag53-specific Th cells on the IgG production against Ag53, we established Ag53-specific Th-cell lines from patients with early onset periodontitis and from healthy volunteers. We then developed a mixed lymphocyte culture system between Ag53-specific Th cells and auto- or alloderived T-cell-depleted leukocytes produced from the subjects whose HLA class II haplotypes were completely matched. Interferon-γ production was observed in all Th cell lines from patients and healthy subjects. As for Th2 type cytokines, interleukin (IL)-4, IL-5, IL-6 and IL-10 production varied greatly in Th cells regardless of the periodontal condition of the donor. Only Th cell lines with a high Th2/Th1 ratio induced Ag53-specific IgG production when cocultured with T-cell-depleted leukocytes. Thus, the difference in Th2/Th1 balance may regulate the Ag53-specific IgG production.

Original languageEnglish
Pages (from-to)112-117
Number of pages6
JournalOral Microbiology and Immunology
Volume20
Issue number2
DOIs
Publication statusPublished - Apr 2005

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Humoral Immunity
Helper-Inducer T-Lymphocytes
Membrane Proteins
Immunoglobulin G
Cell Line
Healthy Volunteers
Leukocytes
Th1-Th2 Balance
Cytokines
Aggressive Periodontitis
T-Lymphocytes
Porphyromonas gingivalis
Periodontitis
Interleukin-5
Interleukin-4
Interleukin-10
Haplotypes
Interferons
Interleukin-6
Molecular Weight

Keywords

  • B cell
  • Class II HLA
  • Cytokine profile
  • IgG antibody
  • Outer membrane protein
  • Periodontitis
  • Porphyromonas gingivalis
  • Th cell line

ASJC Scopus subject areas

  • Immunology
  • Microbiology (medical)
  • Dentistry(all)

Cite this

Role of helper T cells in the humoral immune responses against 53-kDa outer membrane protein from Porphymmonas gingivalis. / Kato, N.; Ohyama, Hideki; Nishimura, F.; Matsushita, S.; Takashiba, Shogo; Murayama, Y.

In: Oral Microbiology and Immunology, Vol. 20, No. 2, 04.2005, p. 112-117.

Research output: Contribution to journalArticle

Kato, N. ; Ohyama, Hideki ; Nishimura, F. ; Matsushita, S. ; Takashiba, Shogo ; Murayama, Y. / Role of helper T cells in the humoral immune responses against 53-kDa outer membrane protein from Porphymmonas gingivalis. In: Oral Microbiology and Immunology. 2005 ; Vol. 20, No. 2. pp. 112-117.
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