Role of fission yeast myosin I in organization of sterol-rich membrane domains

Tetsuya Takeda, Fred Chang

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

Specialized membrane domains containing lipid rafts are thought to be important for membrane processes such as signaling and trafficking [1, 2]. An unconventional type I myosin has been shown to reside in lipid rafts and function to target a disaccharidase to rafts in brush borders of intestinal mammalian cells [3]. In the fission yeast Schizosaccharomyces pombe, distinct sterol-rich membrane domains are formed at the cell division site and sites of polarized cell growth at cell tips [4]. Here, we show that the sole S. pombe myosin I, myo1p, is required for proper organization of these membrane domains. myo1 mutants lacking the TH1 domain exhibit a uniform distribution of sterol-rich membranes all over the plasma membrane throughout the cell cycle. These effects are independent of endocytosis because myo1 mutants exhibit no endocytic defects. Conversely, overexpression of myo1p induces ectopic sterol-rich membrane domains. Myo1p localizes to nonmotile foci that cluster in sterol-rich plasma membrane domains and fractionates with detergent-resistant membranes. Because the myo1p TH1 domain may bind directly to acidic phospholipids, these findings suggest a model for how type I myosin contributes to the organization of specialized membrane domains.

Original languageEnglish
Pages (from-to)1331-1336
Number of pages6
JournalCurrent Biology
Volume15
Issue number14
DOIs
Publication statusPublished - Jul 26 2005
Externally publishedYes

Fingerprint

Myosin Type I
Schizosaccharomyces
Schizosaccharomyces pombe
Sterols
myosin
Yeast
sterols
Membranes
plasma membrane
mutants
Cells
endocytosis
Cell membranes
lipids
microvilli
detergents
cell division
cell growth
cell cycle
phospholipids

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)

Cite this

Role of fission yeast myosin I in organization of sterol-rich membrane domains. / Takeda, Tetsuya; Chang, Fred.

In: Current Biology, Vol. 15, No. 14, 26.07.2005, p. 1331-1336.

Research output: Contribution to journalArticle

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