Role of each immunoglobulin-like loop of nectin for its cell-cell adhesion activity

Masato Yasumi, Kazuya Shimizu, Tomoyuki Honda, Masakazu Takeuchi, Yoshimi Takai

Research output: Contribution to journalArticlepeer-review

47 Citations (Scopus)

Abstract

Nectins are Ca2+-independent immunoglobulin (Ig)-like cell-cell adhesion molecules that form cell-cell junctions, cooperatively with or independently of cadherins, in a variety of cells. Nectins comprise a family of four members, nectin-1, -2, -3, and -4. All nectins have one extracellular region with three Ig-like loops, one transmembrane segment, and one cytoplasmic tail. It has been shown mainly by use of cadherin-deficient L fibroblasts stably expressing each nectin that nectins first form homo-cis-dimers and then homo- or hetero-trans-dimers, causing cell-cell adhesion, and that the formation of the cis-dimers is necessary for the formation of the trans-dimers. However, kinetics of the formation of these dimers have not been examined biochemically by use of pure nectin proteins. We prepared here pure recombinant proteins of extracellular fragments of nectin-3 containing various combinations of Ig-like loops, all of which were fused to the Fc portion of IgG and formed homo-cis-dimers through the Fc portion, and of an extracellular fragment of nectin-1 containing three Ig-like loops which was fused to secreted alkaline phosphatase and formed homo-cis-dimers. We showed here by use of these proteins that the first Ig-like loop of nectin-3 was essential and sufficient for the formation of trans-dimers with nectin-1, but that the second Ig-like loop of nectin-3 was furthermore necessary for its cell-cell adhesion activity.

Original languageEnglish
Pages (from-to)61-66
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume302
Issue number1
DOIs
Publication statusPublished - Feb 28 2003
Externally publishedYes

Keywords

  • Adherens junctions
  • Cell-cell adhesion molecule
  • Immunoglobulin-like loop
  • Nectin

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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