Role of cytochrome b5 in the oxidative metabolism of polychlorinated biphenyls catalyzed by cytochrome P450

K. Matsusue, N. Ariyoshi, K. Oguri, N. Koga, H. Yoshimura

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)

Abstract

The role of cytochrome b5 in the cytochrome P450-dependent hydroxylation of tetrachlorobiphenyl (TCB) isomers was examined using a reconstituted system consisting of CYP2B1 and CYP1A1 and rat liver microsomes. By addition of cytochrome b5 to the reconstituted system containing CYP2B1, the 3-hydroxylation of 2,5,2,'5'- and 2,5,3',4'-TCB was increased about six-fold, but the 3- and 5-hydroxylation of 2,4,3',4'-TCB was decreased by about 50%. All hydroxylations of 3,4,3',4'-, 2,5,3',4'- and 2,4,3',4'-TCBs were decreased by addition of cytochrome b5 to the reconstituted system containing CYP1A1. In stoichiometry measurements, changes in NADPH oxidation and coupling efficiency by addition of cytochrome b5 was observed and these differed according to the position of chlorine atoms of TCBs and cytochrome P450 isoforms used in the systems.

Original languageEnglish
Pages (from-to)405-414
Number of pages10
JournalXenobiotica
Volume26
Issue number4
DOIs
Publication statusPublished - Jan 1 1996
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry
  • Toxicology
  • Pharmacology
  • Health, Toxicology and Mutagenesis

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