Role of chicken Pit-1 isoforms in activating growth hormone gene

Daisuke Murase, Shusuke Taniuchi, Sakae Takeuchi, Hiromi Adachi, Norio Kansaku, Katsuichiro Okazaki, Takeshi Ohkubo

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

In the present study, we expressed chicken (ch) Pit-1α (chPit-1α) and chPit-1γ in vitro to compare the roles of chPit-1s in the transcription of the chicken growth hormone (chGH) gene. Both green fluorescence protein (GFP)-fused chPit-1γ and GFP-fused chPit-1α were localized in the nuclei of COS-7 cells. In a luciferase reporter gene assay, both chPit-1α and chPit-1γ transactivated the chGH promoter, and chPit-1α showed a more potent effect than chPit-1γ. On the other hand, an increase of cellular cAMP induced by forskolin promoted transactivation of the chGH gene with chPit-1α and chPit-1γ to similar extents. These results suggest that chPit-1γ may modulate the basal promoter activity of the chGH gene to the same degree as chPit-1α; however, a structural difference observed at the N-terminus transactivation domains in chPit-1α and chPit-1γ could be associated with the efficiency of basal activation of the chGH promoter.

Original languageEnglish
Pages (from-to)248-252
Number of pages5
JournalGeneral and Comparative Endocrinology
Volume173
Issue number2
DOIs
Publication statusPublished - Sep 1 2011

Keywords

  • Chicken
  • Growth hormone
  • Pit-1
  • Promoter

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Endocrinology

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